| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,173,808 | T→A | D530V (GAT→GTT) | parE ← | DNA topoisomerase IV, subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,173,808 | 0 | T | A | 83.3% | 27.9 / 0.2 | 12 | D530V (GAT→GTT) | parE | DNA topoisomerase IV, subunit B |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base A (5/5); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
CAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGAT > NC_000913/3173675‑3173938 | cAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATa < 1:375624/139‑1 (MQ=255) ttCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTg > 1:605944/1‑139 (MQ=255) aaaCGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGAc < 2:249270/139‑1 (MQ=255) aaTTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACa < 2:605944/139‑1 (MQ=255) cTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAgcg < 1:175414/139‑1 (MQ=255) cGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGAACa > 2:507972/1‑53 (MQ=255) cccTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGAc > 1:126965/1‑77 (MQ=255) cccTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCGCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGAc < 2:126965/77‑1 (MQ=255) cccTCTTTCTCTTCTTCCGCCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGAc > 1:321969/1‑88 (MQ=255) cccTCTTTCTCTTCTTCCGCCAGCGCGTAATAAACCTCTTTCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGAc < 2:321969/88‑1 (MQ=255) cttcCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAg > 1:479677/1‑139 (MQ=255) tCCCGAGAACAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGAt > 2:410442/1‑139 (MQ=255) | CAATTGCATCGGGTTCATTTCCCCCAGACCTTTAAAACGCTGGACGTTCGGCTTGCCTTTCTTGCGTTTTAATTGCTCAAGTACGCCCTCTTTCTCTTCTTCCGTCAGCGCGTAATAAACCTCTTTCCCGAGATCAATACGGTAGAGCGGTGGCAGTGCGACGTAAACGTGACCGTGTTTCACCAACGCGCGGAAATGTTTTACGAACAAAGCGCAGAGCAGCGTGGCAATGTGCAGACCATCAGAGTCCGCATCCGCGAGGAT > NC_000913/3173675‑3173938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |