Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,349,840	T→C	K207R (AAG→AGG)	elbB ←	isoprenoid biosynthesis protein with amidotransferase‑like domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,349,840	0	T	C	100.0%	57.8 / NA	16	K207R (AAG→AGG)	elbB	isoprenoid biosynthesis protein with amidotransferase‑like domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/12);  total (4/12)

CCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGA  >  NC_000913/3349723‑3349936                                                                                                                      |                                                                                                 cccGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCtt                                                                             <  2:500467/139‑1 (MQ=255)             aaCGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCt                                                                 <  2:598028/139‑1 (MQ=255)                       aCAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                       <  2:426894/139‑1 (MQ=255)                       aCAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCat                                                       <  2:551983/139‑1 (MQ=255)                              ttAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCt                                                <  1:231752/139‑1 (MQ=255)                                 aCCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTggg                                             >  1:111548/1‑139 (MQ=255)                                 aCCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTggg                                             <  2:111548/139‑1 (MQ=255)                                     cAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtgg                                         <  2:45657/139‑1 (MQ=255)                                      aaaaGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGggtggt                                        <  1:543110/139‑1 (MQ=255)                                                    aacgaaACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACaa                                   >  1:2383/1‑130 (MQ=255)                                                    aacgaaACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACaa                                   <  2:2383/130‑1 (MQ=255)                                                           cTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTtcatc                   <  1:437115/139‑1 (MQ=255)                                                                   cGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGa           >  1:297022/1‑139 (MQ=255)                                                                       aaGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCGGCCTATCAATGCCGCTCGCCGCTGCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGAGACAATCTTATTGTCTTCATCAACCACGATatc       <  1:611232/139‑1 (MQ=255)                                                                        aGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATatca      >  1:493774/1‑139 (MQ=255)                                                                            gCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCCTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGa  <  2:25573/139‑1 (MQ=255)                                                                                                                      |                                                                                                 CCCGCCCCAGAAAACGGCGAGGACAACCATTAACCGCAAAAGAAAACGGCGAACGAAACTAAACACCGTTAAGCGGCTTTTACTCATTCAGCCAGAACCAGCACGCGGGAAACCAGCTTATCAATGCCGCTCGCCGCTTCTGCAATGTTCTGCGCCAGCATATATGCTGGGGTGGTGACAATCTTATTGTCTTCATCAACCACGATATCATCGA  >  NC_000913/3349723‑3349936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑