Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,182,907 T→C Y555H (TAC→CAC)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,182,9070TC100.0% 157.7 / NA 41Y555H (TAC→CAC) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/24);  total (17/24)

GCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACG  >  NC_000913/4182774‑4183033
                                                                                                                                     |                                                                                                                              
gcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGt                                                                                                                           <  1:177865/139‑1 (MQ=255)
gcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGt                                                                                                                           <  2:220240/139‑1 (MQ=255)
gcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGt                                                                                                                           <  1:524367/139‑1 (MQ=255)
            tATGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCa                                                                                                               <  1:455470/139‑1 (MQ=255)
              tGGACCAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAAt                                                                                                             >  2:466854/1‑139 (MQ=255)
                  ccAGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGaa                                                                                                         <  2:44478/139‑1 (MQ=255)
                    aGAACAACCCGTTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAAc                                                                                                       <  2:399686/139‑1 (MQ=255)
                    aGAACAACCCGCTGTCTGAGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAAc                                                                                                       >  1:481111/1‑139 (MQ=255)
                           cccGCTGTCTGTGATTGCGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGaa                                                                                                <  2:398911/139‑1 (MQ=255)
                                          tgcGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                 <  1:398012/137‑1 (MQ=255)
                                          tgcGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                 <  1:444262/137‑1 (MQ=255)
                                            cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGttt                                                                               >  2:25406/1‑137 (MQ=255)
                                            cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                               >  1:234595/1‑139 (MQ=255)
                                            cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                               >  1:316936/1‑139 (MQ=255)
                                            cGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                               >  2:21814/1‑139 (MQ=255)
                                              cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                 <  1:86112/135‑1 (MQ=255)
                                              cacaAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt                                                                                 >  2:86112/1‑135 (MQ=255)
                                                         tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTg                                                                                                  >  1:424254/1‑107 (MQ=255)
                                                         tATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTg                                                                                                  <  2:424254/107‑1 (MQ=255)
                                                           tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                >  1:32023/1‑139 (MQ=255)
                                                           tctcCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                <  2:32023/139‑1 (MQ=255)
                                                                gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                           <  1:353212/139‑1 (MQ=255)
                                                                gCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgtgt                                                           <  1:183179/139‑1 (MQ=255)
                                                                     cGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGca                                                      <  2:101213/139‑1 (MQ=255)
                                                                      ggCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGcac                                                     >  1:488740/1‑139 (MQ=255)
                                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACa                                                                                      <  1:218465/94‑1 (MQ=255)
                                                                                  cTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACa                                                                                      >  2:218465/1‑94 (MQ=255)
                                                                                              cGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTg                             <  1:609092/139‑1 (MQ=255)
                                                                                                tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc                                                       >  2:205039/1‑111 (MQ=255)
                                                                                                tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc                                                       >  2:165897/1‑111 (MQ=255)
                                                                                                tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc                                                       <  1:165897/111‑1 (MQ=255)
                                                                                                tGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc                                                       <  1:205039/111‑1 (MQ=255)
                                                                                                        tCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt                               >  2:514032/1‑127 (MQ=255)
                                                                                                        tCGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCt                               <  1:514032/127‑1 (MQ=255)
                                                                                                         cGAAGTTCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTAt                  >  1:470131/1‑139 (MQ=255)
                                                                                                               tCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                <  2:211448/87‑1 (MQ=255)
                                                                                                               tCGAGACGTACACCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc                                                                >  1:211448/1‑87 (MQ=255)
                                                                                                                        acacCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                               <  1:518208/63‑1 (MQ=255)
                                                                                                                        acacCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCt                                                                               >  2:518208/1‑63 (MQ=255)
                                                                                                                         cacCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg  <  1:31448/139‑1 (MQ=255)
                                                                                                                         cacCCGACTCACCACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACg  <  2:488740/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                              
GCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTCCTTGAGACTCCGTATCGTAAAGTGACCGACG  >  NC_000913/4182774‑4183033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑