| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,384,861 | G→A | Q341Q (CAG→CAA) | yjeM → | putative transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,384,861 | 0 | G | A | 75.0% | 50.6 / 17.0 | 32 | Q341Q (CAG→CAA) | yjeM | putative transporter |
| Reads supporting (aligned to +/- strand): ref base G (4/4); new base A (12/12); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCG > NC_000913/4384727‑4384994 | gCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGACTTACGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGa > 2:377199/1‑139 (MQ=255) gAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGc < 2:373098/139‑1 (MQ=255) aaGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGcc > 1:447962/1‑139 (MQ=255) tCGCTGGGCGTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGa > 2:372096/1‑139 (MQ=255) gCTGGGCGTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGAcg < 2:121766/139‑1 (MQ=255) gTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGa < 1:161187/139‑1 (MQ=255) ggACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTa < 1:303253/139‑1 (MQ=255) tCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGa > 2:579037/1‑79 (MQ=255) tCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGa < 1:579037/79‑1 (MQ=255) ataCCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACGGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGccg > 1:364700/1‑137 (MQ=255) gTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCa > 1:166330/1‑62 (MQ=255) gTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCa < 2:166330/62‑1 (MQ=255) gCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGcc < 1:465332/104‑1 (MQ=255) gCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGcc > 2:465332/1‑104 (MQ=255) cGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTAc < 1:69060/139‑1 (MQ=255) cGCTTTGCTATTCACCGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCAt < 1:369465/139‑1 (MQ=255) gCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATc > 1:218184/1‑139 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTAt < 1:265963/85‑1 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTAt > 2:265963/1‑85 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGc < 2:415955/88‑1 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGc > 1:415955/1‑88 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGt > 2:93287/1‑102 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGt < 1:93287/102‑1 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGtt > 1:521058/1‑139 (MQ=255) cGTTGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGtt > 2:73727/1‑139 (MQ=255) ttGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGa < 2:280497/94‑1 (MQ=255) ttGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGa > 1:280497/1‑94 (MQ=255) ttGAAAGCCATCATCCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTtg > 2:196899/1‑139 (MQ=255) atcatcCAAGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTa < 1:182114/139‑1 (MQ=255) atcCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCg < 2:218184/139‑1 (MQ=255) aGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggt < 1:507325/90‑1 (MQ=255) aGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGggt > 2:507325/1‑90 (MQ=255) | GCACTGCATTTGTCACCTGAAGCGTCATTGTCGCTGGGCGTATGGTTTGCGCGTATTACTGGACTTTCGATGTTCCTCGCCTATACCGGTGCGTTCTTTACGCTTTGCTATTCACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCG > NC_000913/4384727‑4384994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |