Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,429,264 C→T N110N (AAC→AAT)  fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,2640CT88.2% 83.3 / 5.2 34N110N (AAC→AAT) fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (16/14);  total (19/15)
Fisher's exact test for biased strand distribution p-value = 6.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAATTCCCGGTTAATGGCGT  >  NC_000913/4429134‑4429395
                                                                                                                                  |                                                                                                                                   
aGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGa                                                                                                                             <  1:455185/139‑1 (MQ=255)
                                    cGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgtgt                                                                                         >  1:5076/1‑139 (MQ=255)
                                      aTGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTc                                                                                       >  1:51600/1‑139 (MQ=255)
                                       tGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCa                                                                                      >  1:327145/1‑139 (MQ=255)
                                         gctgctGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCAtt                                                                                    >  1:432691/1‑139 (MQ=255)
                                                            aCCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGa                                                                 >  2:93565/1‑139 (MQ=255)
                                                              cTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgcgc                                                                                                                       >  2:79696/1‑83 (MQ=255)
                                                              cTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgcgc                                                                                                                       <  1:79696/83‑1 (MQ=255)
                                                                 gaagaaAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            <  2:5076/139‑1 (MQ=255)
                                                                 gaagaaAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            <  2:432691/139‑1 (MQ=255)
                                                                 gaagaaAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            <  2:51600/139‑1 (MQ=255)
                                                                 gaagaaAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            <  1:536845/139‑1 (MQ=255)
                                                                 gaagaaAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            <  1:588297/139‑1 (MQ=255)
                                                                   agaaAACGCCAAAAAAGAAGGTGTGAATAGCTCCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCAcc                                                          <  2:486507/139‑1 (MQ=255)
                                                                       aaCGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAAtt                                                                                                                   <  2:374103/78‑1 (MQ=255)
                                                                       aaCGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAAtt                                                                                                                   >  1:374103/1‑78 (MQ=255)
                                                                       aaCGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            >  1:582829/1‑133 (MQ=255)
                                                                       aaCGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCa                                                            <  2:582829/133‑1 (MQ=255)
                                                                           ccAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGa                                                  <  2:365377/139‑1 (MQ=255)
                                                                                      ggTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTg                                       >  2:604876/1‑139 (MQ=255)
                                                                                                 cACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTcc                                           <  2:33255/124‑1 (MQ=255)
                                                                                                 cACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTcc                                           >  1:33255/1‑124 (MQ=255)
                                                                                                 cACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGaa                            >  1:51417/1‑139 (MQ=255)
                                                                                                 cACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGaa                            >  2:141701/1‑139 (MQ=255)
                                                                                                 cACCGAATCTGGCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGaa                            >  2:343766/1‑139 (MQ=255)
                                                                                                 cACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGaa                            >  1:123347/1‑139 (MQ=255)
                                                                                                           ggCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAAt                  >  2:262935/1‑139 (MQ=255)
                                                                                                           ggCCTGCAATTCCGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAAt                  >  2:506832/1‑139 (MQ=255)
                                                                                                           ggCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAAt                  >  2:348197/1‑139 (MQ=255)
                                                                                                                      ccGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                                                                           <  2:304270/55‑1 (MQ=255)
                                                                                                                      ccGCGTGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCgt                                                                                           >  1:304270/1‑55 (MQ=255)
                                                                                                                           tGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                                                                                   >  2:466999/1‑58 (MQ=255)
                                                                                                                           tGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTa                                                                                   <  1:466999/58‑1 (MQ=255)
                                                                                                                           tGATCAATCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAATTCCCGGTTAATGGCGt  <  1:262935/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                   
AGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGCAATTCCGGCACGTACCGACCGCGTTCGTGTTCATTACACCGGTAAACTGATCGACGGCACCGTGTTTGACAGCTCCGTTGCTCGTGGTGAACCCGCTGAATTCCCGGTTAATGGCGT  >  NC_000913/4429134‑4429395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑