Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,476,575 T→C V380A (GTA→GCA)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,476,5750TC90.2% 123.9 / 3.4 41V380A (GTA→GCA) yjgLSopA‑central‑domain‑like hexapeptide repeat protein
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base C (18/19);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

AACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCA  >  NC_000913/4476439‑4476712
                                                                                                                                        |                                                                                                                                         
aaCAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCaa                                                                                                                                         <  1:449024/139‑1 (MQ=255)
   aaTGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGaa                                                                                                                                      >  2:592342/1‑139 (MQ=255)
     tGAAATTAATGCCATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTTGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATa                                                                                                                                    <  2:401982/139‑1 (MQ=255)
     tGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATa                                                                                                                                    >  2:27757/1‑139 (MQ=255)
     tGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATa                                                                                                                                    >  2:349830/1‑139 (MQ=255)
             aTGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTg                                                                                                                            >  1:318930/1‑139 (MQ=255)
             aTGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTg                                                                                                                            <  1:521391/139‑1 (MQ=255)
                 cATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGgtgt                                                                                                                        <  2:318930/139‑1 (MQ=255)
                  aTCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGtt                                                                                                                       >  1:79239/1‑139 (MQ=255)
                           aaCCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGTTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATaaaa                                                                                                              >  2:402360/1‑139 (MQ=255)
                                  ctcATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCa                                                                                                       <  2:79239/139‑1 (MQ=255)
                                           ttATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTtga                                                                                              >  2:236692/1‑139 (MQ=255)
                                                   tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATaaaa                                                                                                              >  2:602388/1‑115 (MQ=255)
                                                   tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATaaaa                                                                                                              <  1:602388/115‑1 (MQ=255)
                                                   tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCaa                                                                                                      <  1:41194/123‑1 (MQ=255)
                                                   tCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCaa                                                                                                      >  2:41194/1‑123 (MQ=255)
                                                     cTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGc                                                                                                            <  1:184012/115‑1 (MQ=255)
                                                     cTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGc                                                                                                            >  2:184012/1‑115 (MQ=255)
                                                         gatgatGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGa                                                                                <  2:574267/139‑1 (MQ=255)
                                                             atgCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATaaa                                                                            >  1:283694/1‑139 (MQ=255)
                                                              tgCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAg                                                                           <  2:283694/139‑1 (MQ=255)
                                                                cAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAgca                                                                         >  2:96943/1‑139 (MQ=255)
                                                                    ggTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGaa                                                                                                                                      >  2:368803/1‑74 (MQ=255)
                                                                    ggTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGaa                                                                                                                                      <  1:368803/74‑1 (MQ=255)
                                                                      ttAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGcatgcatg                                                                   <  1:127007/139‑1 (MQ=255)
                                                                         aCCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTc                                                                <  2:161344/139‑1 (MQ=255)
                                                                                  cGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATaaa                                                       <  2:541460/139‑1 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATc                                                     >  1:279122/1‑139 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGttt                                                                                                                      >  1:565786/1‑74 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGttt                                                                                                                      <  2:565786/74‑1 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTtga                                                                                              <  2:349358/98‑1 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTtga                                                                                              >  1:349358/1‑98 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGa                                                                                <  1:46127/112‑1 (MQ=255)
                                                                                    gTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGa                                                                                >  2:46127/1‑112 (MQ=255)
                                                                                     tCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCa                                                    >  2:211823/1‑139 (MQ=255)
                                                                                              cgattcTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGttt                                                                                                                      >  2:521076/1‑64 (MQ=255)
                                                                                              cgattcTGGAGACTTTTTCAAAATCCCCATATATTAATGATGCAAGAATACTGGATTGGTGttt                                                                                                                      <  1:521076/64‑1 (MQ=255)
                                                                                                                                  atgatgCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTaa                                                                                     <  2:140853/61‑1 (MQ=255)
                                                                                                                                  atgatgCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTaa                                                                                     >  1:140853/1‑61 (MQ=255)
                                                                                                                                  atgatgCAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACattatt       >  2:522613/1‑139 (MQ=255)
                                                                                                                                       gTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCa  >  1:311715/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                         
AACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCA  >  NC_000913/4476439‑4476712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑