| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 713,788 | C→T | I77I (ATC→ATT) | pgm → | phosphoglucomutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 713,788 | 0 | C | T | 89.7% | 73.8 / 2.5 | 29 | I77I (ATC→ATT) | pgm | phosphoglucomutase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (13/13); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01 | |||||||||||
CAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAAC > NC_000913/713655‑713899 | cAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTgg > 2:240824/1‑139 (MQ=255) atggAATGCGGAGCACGCGGTGAAATTCGCTACTCCCGGTCACCGCGGCATCGCATCTCGCCAAAGCTTTAACGAGCCGCACATTCTGGCGATCGCTAAGGCACCTGCTGAAGAACGTGCGAAAAACGGCATTACTGGc < 1:193063/137‑1 (MQ=255) gggAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGcc > 2:45219/1‑139 (MQ=255) gTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGc < 1:45219/139‑1 (MQ=255) tACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGcc < 1:240824/139‑1 (MQ=255) ccGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTc > 1:252110/1‑139 (MQ=255) ggTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCg < 2:27616/139‑1 (MQ=255) aGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGcattca > 2:586876/1‑139 (MQ=255) cAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGcc < 2:5628/116‑1 (MQ=255) cAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGcc > 1:5628/1‑116 (MQ=255) gcgcgcCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCg < 1:586876/139‑1 (MQ=255) cgcgcCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGt > 2:454198/1‑139 (MQ=255) aGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTgg > 1:208988/1‑64 (MQ=255) aGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTgg < 2:208988/64‑1 (MQ=255) ccGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTAtgt > 2:35018/1‑74 (MQ=255) ccGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTAtgt < 1:35018/74‑1 (MQ=255) ccGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGa > 2:88695/1‑139 (MQ=255) cGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGaa < 2:252110/139‑1 (MQ=255) cacaTTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACg < 1:454198/139‑1 (MQ=255) ttCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGt < 1:607469/139‑1 (MQ=255) tGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGa < 1:441221/139‑1 (MQ=255) cGATCGCTCAGGCAATTGCTGAAGAACGTTCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTa < 2:413827/66‑1 (MQ=255) cGATCGCTCAGGCAATTGCTGAAGAACGTTCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTa > 1:413827/1‑66 (MQ=255) cGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGATGTCa > 1:513803/1‑137 (MQ=255) cGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGATGTCa < 2:513803/137‑1 (MQ=255) ttGCTGAAGAACGTGCGAAAAACGGCATTACTGGccc < 2:17270/37‑1 (MQ=255) ttGCTGAAGAACGTGCGAAAAACGGCATTACTGGccc > 1:17270/1‑37 (MQ=255) ttGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGATGTCATTGTGCAGGaaaa > 2:305643/1‑139 (MQ=255) tGCTGAAGAACGTGCGAAAAACGGCATTACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAAc > 1:351076/1‑139 (MQ=255) | CAGGGAATGCGGAGCACGCGGTGAAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCACATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCTTGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAAGTGCTGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAAC > NC_000913/713655‑713899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |