Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,105,536	A→G	A41A (GCA→GCG)	ymdA →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,105,536	0	A	G	90.9%	101.8 / 1.1	33	A41A (GCA→GCG)	ymdA	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (14/16);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

ATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTG  >  NC_000913/1105410‑1105671                                                                                                                               |                                                                                                                                        aTAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGc                                                                                                                             >  2:14746/1‑139 (MQ=255) aTAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGc                                                                                                                             >  2:139072/1‑139 (MQ=255)    aaTGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTTTGGAACCGGCGTGTGACGTCAGCAcc                                                                                                                          >  1:290853/1‑139 (MQ=255)    aaTGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCAcc                                                                                                                          <  1:139072/139‑1 (MQ=255)     aTGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCAccc                                                                                                                         >  1:211414/1‑139 (MQ=255)            gACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGtcatc                                                                                                                  >  2:281412/1‑139 (MQ=255)                 tttttAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCg                                                                                                             <  2:35949/139‑1 (MQ=255)                          tctctTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTTTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGa                                                                                                    <  2:290853/139‑1 (MQ=255)                               tATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGc                                                                                               <  2:196794/139‑1 (MQ=255)                                aTGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGcc                                                                                              >  1:475585/1‑139 (MQ=255)                                  gCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCca                                                                                            <  1:138960/139‑1 (MQ=255)                                   cTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCAcc                                                                                                                          >  2:346343/1‑108 (MQ=255)                                   cTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCAcc                                                                                                                          <  1:346343/108‑1 (MQ=255)                                      ggCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGa                                                                                        >  1:318924/1‑139 (MQ=255)                                      ggCAGTATGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGa                                                                                        >  2:149907/1‑139 (MQ=255)                                       gCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATTGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGaa                                                                                       <  2:475585/139‑1 (MQ=255)                                       gCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGaa                                                                                       <  2:211414/139‑1 (MQ=255)                                       gCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGaa                                                                                       <  2:332202/139‑1 (MQ=255)                                        cAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAAt                                                                                      >  1:133208/1‑139 (MQ=255)                                                  tttCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTc                                                                            <  1:272173/139‑1 (MQ=255)                                                        tttATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCa                                                                      <  2:133208/139‑1 (MQ=255)                                                                   tGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTcc                                                           >  1:459274/1‑139 (MQ=255)                                                                       ggAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCa                                                       <  1:251963/139‑1 (MQ=255)                                                                             aCCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGt                                                 <  2:556154/139‑1 (MQ=255)                                                                                gTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTaa                                              >  2:142899/1‑139 (MQ=255)                                                                                          gCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGa                                                                                                    >  2:175868/1‑75 (MQ=255)                                                                                          gCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGa                                                                                                    <  1:175868/75‑1 (MQ=255)                                                                                                 ccATTTTTATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACCCCAGCAAAGCGTTAATGAGCGGCAATGTCaaa                             <  1:34310/139‑1 (MQ=255)                                                                                                     ttttATGGCCAAATTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAAcg                         <  1:281412/139‑1 (MQ=255)                                                                                                                aaTTGTGGAACCGGCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGcc              <  2:459274/139‑1 (MQ=255)                                                                                                                       gAACCGGCGTGCGACGTCAGCACCCGGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATTTCAAAAACGCGCAAATAGCCTCAGTaa       <  2:398608/139‑1 (MQ=255)                                                                                                                          ccGGCGTGTGACGTCAGCACCCAGTCAACACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAg    >  1:60075/1‑139 (MQ=255)                                                                                                                            ggCGTGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTg  <  1:142899/139‑1 (MQ=255)                                                                                                                               |                                                                                                                                        ATAAATGTTCAGACCTTTTTTAAACTCTCTTATGCTCGGCAGTTTGTTTTTTCCTTTTATTGCCATTGCTGGAAGCACCGTGCAAGGGGGCGTGATCCATTTTTATGGCCAAATTGTGGAACCGGCATGTGACGTCAGCACCCAGTCATCACCCGTAGAAATGAACTGCCCACAGAATGGTTCTATTCCGGGCAAAACCTACTCCAGCAAAGCGTTAATGAGCGGCAATGTCAAAAACGCGCAAATAGCCTCAGTAAAAGTG  >  NC_000913/1105410‑1105671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑