Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,867,926 (C)6→7 coding (828/1140 nt) nlpD ← activator of AmiC murein hydrolase activity, lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,867,9201.C96.8% 228.7 / ‑0.7 63coding (834/1140 nt)nlpDactivator of AmiC murein hydrolase activity, lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (31/30);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCG  >  NC_000913/2867783‑2868044
                                                                                                                                          |                                                                                                                              
attaGGTAATTCTTATTTTGTTTGAATATTATCCGAATAACGTAGGCCCGCCACCCCGTAACAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               <  2:581724/136‑1 (MQ=255)
     gTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTGGCGATAATTACCTGTCCTTGGCTGCCTACAATAACAATCCCCTTGTTAcccccc                                                                                                                        >  1:581724/1‑139 (MQ=255)
       aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               >  1:1274771/1‑132 (MQ=255)
       aaTCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               <  2:1274771/132‑1 (MQ=255)
           atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCCATCCCCTTGTTGCCCCCCCTCAGa                                                                                                                   >  2:907016/1‑139 (MQ=255)
           atcatTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGa                                                                                                                   >  1:1507128/1‑139 (MQ=255)
             catTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAg                                                                                                                 >  2:1359663/1‑139 (MQ=255)
              atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               >  1:1717002/1‑125 (MQ=255)
              atTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               <  2:1717002/125‑1 (MQ=255)
               ttATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAgcg                                                                                                               >  2:464208/1‑139 (MQ=255)
                  tGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCa                                                                                                            <  1:237881/139‑1 (MQ=255)
                     ttGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAg                                                                                                         <  2:1400436/139‑1 (MQ=255)
                      tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            <  1:485022/119‑1 (MQ=255)
                      tgatgaTAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            >  2:485022/1‑119 (MQ=255)
                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGt                                                                                                       >  1:1138287/1‑139 (MQ=255)
                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGt                                                                                                       >  1:189757/1‑139 (MQ=255)
                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               >  2:1089217/1‑116 (MQ=255)
                       gatgatAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               <  1:1089217/116‑1 (MQ=255)
                            tAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  2:1626331/1‑139 (MQ=255)
                              aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  <  1:129471/137‑1 (MQ=255)
                              aTCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  2:129471/1‑137 (MQ=255)
                                      aCCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGcc                                                                                        <  2:1138287/139‑1 (MQ=255)
                                        cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      <  1:1195975/139‑1 (MQ=255)
                                        cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      >  1:1288700/1‑139 (MQ=255)
                                          tggCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            <  1:1071760/97‑1 (MQ=255)
                                          tAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            >  2:1071760/1‑99 (MQ=255)
                                             ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAg                                                                                                         >  1:691937/1‑115 (MQ=255)
                                             ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAg                                                                                                         <  2:691937/115‑1 (MQ=255)
                                             ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTc                                                                                 >  2:1217741/1‑139 (MQ=255)
                                                cgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGc                                                                              <  1:907016/139‑1 (MQ=255)
                                                 gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      <  2:382077/130‑1 (MQ=255)
                                                 gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTCTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCACCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCt                                                                                      >  1:382077/1‑130 (MQ=255)
                                                 gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCa                                                                            >  2:846226/1‑139 (MQ=255)
                                                  cAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               >  1:347720/1‑89 (MQ=255)
                                                  cAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               <  2:347720/89‑1 (MQ=255)
                                                     cgcgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcg                                                                         <  2:239495/139‑1 (MQ=255)
                                                             cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcgc                                                                        >  2:608375/1‑132 (MQ=255)
                                                             cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcgc                                                                        <  1:608375/132‑1 (MQ=255)
                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGaa                                                                                                                  <  2:815830/83‑1 (MQ=255)
                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGaa                                                                                                                  >  1:815830/1‑83 (MQ=255)
                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGt                                                                                                       <  1:1276868/94‑1 (MQ=255)
                                                                    aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGt                                                                                                       >  2:1276868/1‑94 (MQ=255)
                                                                     acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                                             <  2:55643/103‑1 (MQ=255)
                                                                     acaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                                             >  1:55643/1‑103 (MQ=255)
                                                                         cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTAc                                                     >  1:1445931/1‑139 (MQ=255)
                                                                         cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTAc                                                     >  1:196358/1‑139 (MQ=255)
                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                    >  2:370517/1‑139 (MQ=255)
                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                    <  2:196358/139‑1 (MQ=255)
                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACt                                                    <  1:1626331/139‑1 (MQ=255)
                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            <  1:1675851/67‑1 (MQ=255)
                                                                          gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            >  2:1675851/1‑67 (MQ=255)
                                                                             gCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGt                                                 <  2:1507128/139‑1 (MQ=255)
                                                                             gCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            >  2:1751380/1‑64 (MQ=255)
                                                                             gCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGccc                                                                                                                            <  1:1751380/64‑1 (MQ=255)
                                                                              ccATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGtt                                                >  2:414871/1‑139 (MQ=255)
                                                                                 tCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGt                                                                   >  2:1536851/1‑117 (MQ=255)
                                                                                 tCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGt                                                                   <  1:1536851/117‑1 (MQ=255)
                                                                                          gcgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               <  2:608136/49‑1 (MQ=255)
                                                                                          gcgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGc                                                                                                                               >  1:608136/1‑49 (MQ=255)
                                                                                            gATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  <  1:831006/75‑1 (MQ=255)
                                                                                            gATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  2:831006/1‑75 (MQ=255)
                                                                                                  tGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCgg                            <  1:137398/139‑1 (MQ=255)
                                                                                                      tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgct                                     <  1:713744/126‑1 (MQ=255)
                                                                                                      tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgct                                     >  2:713744/1‑126 (MQ=255)
                                                                                                        tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCt                          >  1:752646/1‑135 (MQ=255)
                                                                                                        tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCt                          <  2:752646/135‑1 (MQ=255)
                                                                                                        tCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCGCCCCCTCAGCAGCGCCAAAGGTTTCTATCATCTTGCCCTCAGGCGGCCAGCGCCAGGTGGAGATAGGCGTACGGGTTGCTGTGCTGCTGACAGTCGGCTCGGt                      >  1:1727903/1‑139 (MQ=255)
                                                                                                               ctgcctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgctt               <  2:189757/139‑1 (MQ=255)
                                                                                                                  cctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                                                           >  1:619354/1‑92 (MQ=255)
                                                                                                                  cctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                                                           <  2:619354/92‑1 (MQ=255)
                                                                                                                  cctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTa                                          <  2:35817/109‑1 (MQ=255)
                                                                                                                  cctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTa                                          >  1:35817/1‑109 (MQ=255)
                                                                                                                         tatCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCgg                       >  2:198199/1‑121 (MQ=255)
                                                                                                                         tatCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCgg                       <  1:198199/121‑1 (MQ=255)
                                                                                                                         tatCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTa     <  2:1445931/139‑1 (MQ=255)
                                                                                                                            cAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCg  >  1:356828/1‑139 (MQ=255)
                                                                                                                               tCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGAt                                                                                                 <  1:685295/41‑1 (MQ=255)
                                                                                                                               tCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGAt                                                                                                 >  2:685295/1‑41 (MQ=255)
                                                                                                                               tCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTgg       >  1:552810/1‑131 (MQ=255)
                                                                                                                               tCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTgg       <  2:552810/131‑1 (MQ=255)
                                                                                                                                   cTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  >  1:1562773/1‑36 (MQ=255)
                                                                                                                                   cTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGa                                                                                                  <  2:1562773/36‑1 (MQ=255)
                                                                                                                                   cTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgct                <  2:649926/118‑1 (MQ=255)
                                                                                                                                   cTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGtgct                >  1:649926/1‑118 (MQ=255)
                                                                                                                                          |                                                                                                                              
CTCAGGTAATCATCATTATGTTTGATGATAATCAGATTACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGTCGGCTCGGTTGTGCTTGCTGTTGGTACCG  >  NC_000913/2867783‑2868044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: