New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4226628 = | 72 (0.970) | 3 (0.040) | 3/206 | 7.3 | 4.2% | coding (2801/3684 nt) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
? | NC_000913 | 4226644 = | 69 (0.970) | coding (2817/3684 nt) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent | |||||
Rejected: Coverage evenness skew score above cutoff. | |||||||||||
Rejected: Frequency below/above cutoff threshold. |
CGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGG > NC_000913/4226494‑4226752 | cgtacgtaTTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGaa > 2:871081/1‑139 (MQ=255) cgtacgtaTTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGaa > 2:1567564/1‑139 (MQ=255) cgtacgtaTTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGaa > 2:531193/1‑139 (MQ=255) cgtacgtaTTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGaa > 1:1482455/1‑139 (MQ=255) aTTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCa > 1:213502/1‑139 (MQ=255) tCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATc > 1:816686/1‑139 (MQ=255) cACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGaaa < 2:1564218/139‑1 (MQ=255) cACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGaaa > 2:680912/1‑139 (MQ=255) aCGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAAc < 1:1567564/139‑1 (MQ=255) gagacgcAAAAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACg < 2:597597/135‑1 (MQ=255) cGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACg < 1:871081/139‑1 (MQ=255) ggCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCt > 2:1319186/1‑139 (MQ=255) gcgcAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg < 1:531193/139‑1 (MQ=255) cgcAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACg > 1:1557831/1‑135 (MQ=255) cgcAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACg < 2:1557831/135‑1 (MQ=255) cgcAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGc > 2:1278156/1‑139 (MQ=255) cgcAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGc > 1:66566/1‑139 (MQ=255) gcAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCg < 2:66566/139‑1 (MQ=255) cgcgCACACCACCCGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACAt < 1:1695542/139‑1 (MQ=255) cgcACACCACGGGACACGCTGGGAGCGGCGCGCGATAACGATTTCTCTTTTGACTGGCGGGCTTACACCCCGCCGGTGGCGCACCGTCGCGGCGTGCGGGAAGGCGGACCCAGCATCGAAACGCCGTGTAATCActgtc > 1:597597/1‑135 (MQ=255) accaccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAg < 2:1127776/108‑1 (MQ=255) accaccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAg > 1:1127776/1‑108 (MQ=255) ccaccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGa < 2:1732962/139‑1 (MQ=255) caccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGac < 2:1272522/139‑1 (MQ=255) caccGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGac < 2:1482455/139‑1 (MQ=255) ggTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACg > 1:206396/1‑112 (MQ=255) ggTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACg < 2:206396/112‑1 (MQ=255) gTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGt < 1:1269803/139‑1 (MQ=255) tCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTAc > 2:1508187/1‑122 (MQ=255) tCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTAc < 1:1508187/122‑1 (MQ=255) aCGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAAt < 2:1427926/117‑1 (MQ=255) aCGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAAt > 1:1427926/1‑117 (MQ=255) aaGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGaca < 1:891436/125‑1 (MQ=255) aaGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGaca > 2:891436/1‑125 (MQ=255) ggcgCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTgg > 2:1681420/1‑139 (MQ=255) gcgcgcGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAg > 2:1024714/1‑86 (MQ=255) gcgcgcGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAg < 1:1024714/86‑1 (MQ=255) gcgcgcGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg < 1:707234/99‑1 (MQ=255) gcgcgcGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg > 2:707234/1‑99 (MQ=255) gcgcgcGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGa < 2:1209074/113‑1 (MQ=255) gcgcgcGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGt > 2:670276/1‑139 (MQ=255) gcgcgcGATAACGATTTCGCTTTTAACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGa > 1:1209074/1‑113 (MQ=255) cgcgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTc > 2:1525732/1‑139 (MQ=255) cgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGc < 1:680912/139‑1 (MQ=255) cgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGc < 1:1620039/139‑1 (MQ=255) cgcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGc < 1:1681420/139‑1 (MQ=255) cgcgATAACGATTTCGCTTTTGACTGGCAAGCTTACACGCCGCCGGTGGTGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGc < 1:1319186/139‑1 (MQ=255) gcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTgg < 1:86615/113‑1 (MQ=255) gcgATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTgg > 2:86615/1‑113 (MQ=255) gATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGa < 1:1278156/139‑1 (MQ=255) aTTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGacac < 1:236273/108‑1 (MQ=255) aTTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGacac > 2:236273/1‑108 (MQ=255) ttGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCcgcg < 2:213502/139‑1 (MQ=255) ggCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCt < 2:1512502/139‑1 (MQ=255) cTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGc > 2:146827/1‑49 (MQ=255) cTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGc < 1:146827/49‑1 (MQ=255) acacGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGt > 2:1698794/1‑87 (MQ=255) acacGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGt < 1:1698794/87‑1 (MQ=255) gccgccGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAgtg > 1:1031845/1‑139 (MQ=255) cgccgGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg < 2:656717/56‑1 (MQ=255) cgccgGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTg > 1:656717/1‑56 (MQ=255) cgccgGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCg < 1:1500856/80‑1 (MQ=255) cgccgGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCg > 2:1500856/1‑80 (MQ=255) cgccgGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAgtggt > 2:353860/1‑139 (MQ=255) ccgGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGtggtgg > 1:204463/1‑139 (MQ=255) gcgcACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGt > 2:728649/1‑73 (MQ=255) gcgcACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGt < 1:728649/73‑1 (MQ=255) cGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCgg < 2:300553/139‑1 (MQ=255) cGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCgg < 2:504751/139‑1 (MQ=255) cGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCgg < 2:821798/139‑1 (MQ=255) cAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGaca < 1:1668255/51‑1 (MQ=255) cAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGaca > 2:1668255/1‑51 (MQ=255) | CGTACGTATTCAGCACGGGCGCAAGAAACCGCGCACACCACCGGTCACGCTGGAAGCGGCGCGCGATAACGATTTCGCTTTTGACTGGCAGGCTTACACGCCGCCGGTGGCGCACCGTCTCGGCGTGCAGGAAGTCGAAGCCAGCATCGAAACGCTGCGTAATTACATCGACTGGACACCGTTCTTTATGACCTGGTCGCTGGCCGGGAAGTATCCGCGCATTCTGGAAGATGAAGTGGTGGGCGTTGAGGCGCAGCGG > NC_000913/4226494‑4226752 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |