Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,112,317 A→G K485K (AAA→AAG opgH → OPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase, nutrient‑dependent cell size regulator, FtsZ assembly antagonist

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,112,3170AG100.0% 196.9 / NA 52K485K (AAA→AAGopgHOPG biosynthetic ACP‑dependent transmembrane UDP‑glucose beta‑1,2 glycosyltransferase, nutrient‑dependent cell size regulator, FtsZ assembly antagonist
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/25);  total (27/25)

GGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTAT  >  NC_000913/1112189‑1112448
                                                                                                                                |                                                                                                                                   
ggTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGAccgcc                                                                                                                           >  1:1712223/1‑139 (MQ=255)
  ttCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGa                                                                                                                         <  2:1231926/139‑1 (MQ=255)
  ttCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGa                                                                                                                         >  1:1410657/1‑139 (MQ=255)
       tCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTg                                                                                                                    <  2:1504724/139‑1 (MQ=255)
            tCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACg                                                                                                               >  2:1733232/1‑139 (MQ=255)
             cacaTGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACgg                                                                                                              <  2:1596242/139‑1 (MQ=255)
              acaTGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGt                                                                                                             <  1:1733232/139‑1 (MQ=255)
               caTGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTa                                                                                                            <  2:507908/139‑1 (MQ=255)
                                  cgTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCt                                                                                         <  1:1401082/139‑1 (MQ=255)
                                    ttGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTCCCGTCTGt                                                                                       <  2:1730472/139‑1 (MQ=255)
                                           gcCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGt                                                                                <  2:1018323/139‑1 (MQ=255)
                                                            gTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGAt                                                                                                                        <  2:53595/82‑1 (MQ=255)
                                                            gTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGAt                                                                                                                        >  1:53595/1‑82 (MQ=255)
                                                              cTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGtt                                                             >  1:256847/1‑139 (MQ=255)
                                                              cTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGtt                                                             >  1:1027177/1‑139 (MQ=255)
                                                              cTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGAAGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGa                                                                              <  2:525738/122‑1 (MQ=255)
                                                              cTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGAAGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGa                                                                              >  1:525738/1‑122 (MQ=255)
                                                                     gCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTg                                                      <  2:1410657/139‑1 (MQ=255)
                                                                     gCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTg                                                      <  1:564838/139‑1 (MQ=255)
                                                                      cTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTc                                                                                     <  2:1228130/107‑1 (MQ=255)
                                                                      cTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTc                                                                                     >  1:1228130/1‑107 (MQ=255)
                                                                      cTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGc                                                     >  1:583410/1‑139 (MQ=255)
                                                                       ttACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCg                                                    >  2:602038/1‑139 (MQ=255)
                                                                                cccGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTg                                           <  2:256847/139‑1 (MQ=255)
                                                                                cccGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTg                                           >  1:708900/1‑139 (MQ=255)
                                                                                 ccGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACg                                                                                                               <  1:428997/70‑1 (MQ=255)
                                                                                 ccGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACg                                                                                                               >  2:428997/1‑70 (MQ=255)
                                                                                 ccGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGa                                                                              <  1:1050587/103‑1 (MQ=255)
                                                                                 ccGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGa                                                                              >  2:1050587/1‑103 (MQ=255)
                                                                                   gggTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTACCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACg                                        >  2:949027/1‑139 (MQ=255)
                                                                                         ttATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTg                                  >  2:1568812/1‑139 (MQ=255)
                                                                                           aTGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCgg                                                   <  2:35697/120‑1 (MQ=255)
                                                                                           aTGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCgg                                                   >  1:35697/1‑120 (MQ=255)
                                                                                           aTGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGAt                                >  2:127967/1‑139 (MQ=255)
                                                                                               agaaTTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCt                            <  1:949027/139‑1 (MQ=255)
                                                                                               agaaTTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCt                            >  1:36423/1‑139 (MQ=255)
                                                                                                gaaTTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGt                                                                                <  1:175362/86‑1 (MQ=255)
                                                                                                gaaTTGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGt                                                                                >  2:175362/1‑86 (MQ=255)
                                                                                                   ttGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTAtc                        >  2:176152/1‑139 (MQ=255)
                                                                                                   ttGCCGCCTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTAtc                        >  1:795622/1‑139 (MQ=255)
                                                                                                      ccgccTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTg                                                      <  2:1293749/106‑1 (MQ=255)
                                                                                                      ccgccTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTg                                                      >  1:1293749/1‑106 (MQ=255)
                                                                                                      ccgccTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTAtctct                     >  1:203707/1‑139 (MQ=255)
                                                                                                         ccTAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTctccg                  <  1:602038/139‑1 (MQ=255)
                                                                                                           tAACTTGCTTGATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTctccgct                <  1:1112130/139‑1 (MQ=255)
                                                                                                                tgcttcATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTccgctccgct           <  2:1110042/139‑1 (MQ=255)
                                                                                                                  cttgATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAg                                                                            <  1:484067/72‑1 (MQ=255)
                                                                                                                  cttgATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAg                                                                            >  2:484067/1‑72 (MQ=255)
                                                                                                                  cttgATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCtgt         >  1:1348633/1‑139 (MQ=255)
                                                                                                                   ttgATGAGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCtgtg        >  1:1147098/1‑139 (MQ=255)
                                                                                                                         aGCTAAAGCGTGACCGCCGATGGTGGCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCAGCTGTGGTTTAt  >  1:1064769/1‑139 (MQ=255)
                                                                                                                         aGCTAAAGCGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTAt  <  2:203707/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                   
GGTTCAATCCTGTCACATGACTTCGTGGAAGCGGCGTTGATGCGCCGTGCAGGTTGGGGGGTCTGGATTGCTTACGATCTCCCGGGTTCTTATGAAGAATTGCCGCCTAACTTGCTTGATGAGCTAAAACGTGACCGCCGATGGTGCCACGGTAACCTGATGAACTTCCGTCTGTTCCTGGTGAAGGGTATGCACCCGGTTCACCGTGCGGTGTTCCTGACGGGCGTGATGTCTTATCTCTCCGCTCCGCTGTGGTTTAT  >  NC_000913/1112189‑1112448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: