Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,249,389	G→A	A123A (GCC→GCT)	dhaL ←	dihydroxyacetone kinase, C‑terminal domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,249,389	0	G	A	100.0%	213.8 / NA	61	A123A (GCC→GCT)	dhaL	dihydroxyacetone kinase, C‑terminal domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/30);  total (31/30)

CTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGA  >  NC_000913/1249255‑1249515                                                                                                                                       |                                                                                                                               cTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCtt                                                                                                                            >  2:1383908/1‑139 (MQ=255)      tgCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCcg                                                                                                                       >  2:1181197/1‑139 (MQ=255)        cAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCcgcg                                                                                                                     <  1:966685/139‑1 (MQ=255)         aGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGa                                                                                                                    <  1:902699/139‑1 (MQ=255)         aGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGa                                                                                                                    <  2:927767/139‑1 (MQ=255)          gCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGAc                                                                                                                   <  2:267759/139‑1 (MQ=255)           cGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACt                                                                                                                  >  2:280651/1‑139 (MQ=255)                  ggCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                           >  2:101643/1‑139 (MQ=255)                        gCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTcc                                                                                                     >  2:1163812/1‑139 (MQ=255)                            cTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCgcgc                                                                                                 <  1:1201742/139‑1 (MQ=255)                              ggCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCa                                                                                               <  1:1383908/139‑1 (MQ=255)                                 aGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                           <  2:887176/124‑1 (MQ=255)                                 aGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                           >  1:887176/1‑124 (MQ=255)                                 aGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcg                                                                                            >  2:172698/1‑139 (MQ=255)                                   cTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                          >  2:794858/1‑139 (MQ=255)                                   cTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACAC‑‑CACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                          <  2:149001/137‑1 (MQ=255)                                   cTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACAC‑‑CACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                          >  1:149001/1‑137 (MQ=255)                                       gAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAAc                                                                                      >  1:649099/1‑139 (MQ=255)                                                        agagaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                     <  2:649099/139‑1 (MQ=255)                                                        agagaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                     <  1:321980/139‑1 (MQ=255)                                                          agaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                           >  2:942256/1‑99 (MQ=255)                                                          agaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACg                                                                                                           <  1:942256/99‑1 (MQ=255)                                                          agaTTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCa                                                                   >  1:611691/1‑139 (MQ=255)                                                             ttttGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtg                                                                >  1:935710/1‑139 (MQ=255)                                                              tttGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAgtgt                                                               >  2:933506/1‑139 (MQ=255)                                                                tGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCa                                                             >  2:881360/1‑139 (MQ=255)                                                                 gCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAg                                                            >  2:202211/1‑139 (MQ=255)                                                                    cGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCt                                                         <  1:101643/139‑1 (MQ=255)                                                                        ggACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                     <  1:1163812/139‑1 (MQ=255)                                                                        ggACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGc                                                     <  1:202211/139‑1 (MQ=255)                                                                         gACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGcc                                                    <  2:387994/139‑1 (MQ=255)                                                                          aCTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCg                                                   <  2:159780/139‑1 (MQ=255)                                                                            tGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTg                                                 <  1:645407/139‑1 (MQ=255)                                                                                 tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTgg                                            <  1:1271134/139‑1 (MQ=255)                                                                                 tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTgg                                            >  2:510914/1‑139 (MQ=255)                                                                                 tAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTgg                                            <  1:172698/139‑1 (MQ=255)                                                                                        tCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCt                                     >  2:1533089/1‑139 (MQ=255)                                                                                                cGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                             <  2:1387871/139‑1 (MQ=255)                                                                                                cGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                             <  1:360291/139‑1 (MQ=255)                                                                                                cGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCACCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTgcggcg                             <  1:1181197/139‑1 (MQ=255)                                                                                                  gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                         >  1:1589470/1‑77 (MQ=255)                                                                                                  gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCgg                                                                                         <  2:1589470/77‑1 (MQ=255)                                                                                                  gCACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGcgcg                           >  1:853223/1‑139 (MQ=255)                                                                                                   cACCCACACATCACACATGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCgg                          <  1:1533089/139‑1 (MQ=255)                                                                                                             tcacacatGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAgg                >  2:1448828/1‑139 (MQ=255)                                                                                                                   atGGTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGa          >  1:1689448/1‑139 (MQ=255)                                                                                                                      gTTTTATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACa       >  2:751636/1‑139 (MQ=255)                                                                                                                        tttATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGc     >  2:130759/1‑139 (MQ=255)                                                                                                                          tATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCg           <  1:1496042/131‑1 (MQ=255)                                                                                                                          tATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCg           >  2:1496042/1‑131 (MQ=255)                                                                                                                          tATCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCgg   <  1:510914/139‑1 (MQ=255)                                                                                                                           aTCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCa                                                                                               >  1:1241591/1‑46 (MQ=255)                                                                                                                           aTCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCa                                                                                               <  2:1241591/46‑1 (MQ=255)                                                                                                                           aTCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGa  >  2:987105/1‑139 (MQ=255)                                                                                                                           aTCGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGa  >  1:213315/1‑139 (MQ=255)                                                                                                                             cGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                          <  2:1408203/49‑1 (MQ=255)                                                                                                                             cGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATcgcg                                                                                          >  1:1408203/1‑49 (MQ=255)                                                                                                                             cGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                     <  2:162173/70‑1 (MQ=255)                                                                                                                             cGCCAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                     >  1:162173/1‑70 (MQ=255)                                                                                                                               ccAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                     <  2:1094624/68‑1 (MQ=255)                                                                                                                               ccAGGTTCAGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTc                                                                     >  1:1094624/1‑68 (MQ=255)                                                                                                                                       |                                                                                                                               CTTTGTGCAGCGGATTCGGCGATGCTACTGGCAGCTTCGAGCGCCACCGGAACAGAGAGATTTTGCTCGCTGGACTGACGTAACGATTCCACCACCGGCACCCACACATCACACATGGTTTTATCGCCAGGTTCGGCTTTCCCGCGACTGATTACGCCGTCCGCGCCATCGCGGAACATCTGATAAAGCTCTTCCAGTGTCAGGCTTTGCCGTGCCTGGGTCGCCTGTGCGGCGCGGATAAAGAAGGTACCGAACAGCGGA  >  NC_000913/1249255‑1249515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑