Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,719	A→G	F300F (TTT→TTC)	narX ←	sensory histidine kinase in two‑component regulatory system with NarL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,719	0	A	G	100.0%	215.9 / NA	57	F300F (TTT→TTC)	narX	sensory histidine kinase in two‑component regulatory system with NarL
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/31);  total (26/31)

TGCGTATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAA  >  NC_000913/1276600‑1276847                                                                                                                        |                                                                                                                                 tGCGTATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCtt                                                                                                               <  2:1118307/139‑1 (MQ=255) tGCGTATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCtt                                                                                                               <  1:1644243/139‑1 (MQ=255) tGCGTATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCtt                                                                                                               >  1:1761727/1‑139 (MQ=255)   cGTATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtca                                                                                                             >  2:145309/1‑139 (MQ=255)     tATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATtctc                                                                                                                 <  2:638830/133‑1 (MQ=255)     tATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATtctc                                                                                                                 >  1:638830/1‑133 (MQ=255)       tGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatcat                                                                                                         <  2:700728/139‑1 (MQ=255)       tGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatcat                                                                                                         <  2:1761727/139‑1 (MQ=255)       tGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatcat                                                                                                         <  1:716144/139‑1 (MQ=255)         agagTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatcatca                                                                                                       <  2:455721/139‑1 (MQ=255)             tCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCGTCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAgtgt                                                                                                   >  1:1297198/1‑139 (MQ=255)                gccagccGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCt                                                                                                                           <  2:1341770/112‑1 (MQ=255)                gccagccGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCt                                                                                                                           >  1:1341770/1‑112 (MQ=255)                            ttCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAAtt                                                                                    <  2:898566/139‑1 (MQ=255)                            ttCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCCCTTCATCATCAGTGTCATACACCCGCAAtt                                                                                    <  1:612968/139‑1 (MQ=255)                            ttCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACCCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAAtt                                                                                    <  2:1467198/139‑1 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCtga                                                                                                                         <  2:1619771/92‑1 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCtga                                                                                                                         >  1:1619771/1‑92 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatc                                                                                                           >  2:1385901/1‑106 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatc                                                                                                           <  1:1385901/106‑1 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtc                                                                                                              >  2:59974/1‑103 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtc                                                                                                              <  1:59974/103‑1 (MQ=255)                                      tGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACg                                                                          >  2:601339/1‑139 (MQ=255)                                                aCCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTGATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATAGCACGTAGCAgggct                                                                >  1:1778973/1‑137 (MQ=255)                                                       ggTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCt                                                         <  1:28145/139‑1 (MQ=255)                                                        gTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTg                                                        >  1:1326288/1‑139 (MQ=255)                                                          aaTACGCCGCGCGGGCAGAGCTGGCAGCCTTTATTATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAg                                                                                                      <  2:398474/91‑1 (MQ=255)                                                          aaTACGCCGCGCGGGCAGAGCTGGCAGCCTTTATTATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAg                                                                                                      >  1:398474/1‑91 (MQ=255)                                                          aaTACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatc                                                                                                           <  1:55453/86‑1 (MQ=255)                                                          aaTACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTtcatc                                                                                                           >  2:55453/1‑86 (MQ=255)                                                            tACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTc                                                                                                                             >  1:435570/1‑66 (MQ=255)                                                            tACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTc                                                                                                                             <  2:435570/66‑1 (MQ=255)                                                                  gcgcgGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACt                                                                                                                              >  1:493600/1‑59 (MQ=255)                                                                  gcgcgGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACt                                                                                                                              <  2:493600/59‑1 (MQ=255)                                                                  gcgcgGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACt                                                                                                                              >  2:1785255/1‑59 (MQ=255)                                                                  gcgcgGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACt                                                                                                                              <  1:1785255/59‑1 (MQ=255)                                                                  gcgcgGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGa                                                                                 >  2:720382/1‑104 (MQ=255)                                                                  gcgcgGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGa                                                                                 <  1:720382/104‑1 (MQ=255)                                                                                        ttATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATa                                                                                               >  1:1061144/1‑68 (MQ=255)                                                                                        ttATCATCACAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATa                                                                                               <  2:1061144/68‑1 (MQ=255)                                                                                           tcatcaCAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGAtt                                                                                                                    >  2:482689/1‑44 (MQ=255)                                                                                           tcatcaCAAGTCATATCTGGCTGGCAGGTGAACTCCTGATGAtt                                                                                                                    <  1:482689/44‑1 (MQ=255)                                                                                                acaAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTaaa                                                             >  1:636258/1‑94 (MQ=255)                                                                                                acaAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTaaa                                                             <  2:636258/94‑1 (MQ=255)                                                                                                 caAGTCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCgggg               <  1:145309/139‑1 (MQ=255)                                                                                                     tCATATCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCGGGGcgcg           <  1:825822/139‑1 (MQ=255)                                                                                                         atgtggctggcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACg                                                                          >  1:1037291/4‑72 (MQ=255)                                                                                                         atCTGGCTGGCAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACg                                                                          <  2:1037291/72‑1 (MQ=255)                                                                                                              gctggcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCaa  >  1:1364000/1‑139 (MQ=255)                                                                                                                  gcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCa                                                                            >  2:301213/1‑61 (MQ=255)                                                                                                                  gcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCa                                                                            <  1:301213/61‑1 (MQ=255)                                                                                                                  gcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAg                                                    <  1:1142868/85‑1 (MQ=255)                                                                                                                  gcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAg                                                    >  2:1142868/1‑85 (MQ=255)                                                                                                                  gcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTa                                          >  2:183109/1‑95 (MQ=255)                                                                                                                  gcAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTa                                          <  1:183109/95‑1 (MQ=255)                                                                                                                   cAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGa                                                                                 <  2:1404214/55‑1 (MQ=255)                                                                                                                   cAGGTGAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGa                                                                                 >  1:1404214/1‑55 (MQ=255)                                                                                                                        |                                                                                                                                 TGCGTATGAGAGTCAGCCAGCCGCCACTTCAGGGTCGTGCCGCGATCACCAACGGGTAATACGCCGCGCGGGCAGAGCTGGCAGCCTTTATCATCACAAGTCATATCTGGCTGGCAGGTAAACTCCTGATGATTCTCTTCATCATCAGTGTCATACACCCGCAATTCGATATCACGTAGCAGGGTTAAATTCTGTAAGCCGTTGAGTACAGGTGACAGGCGTTCACACAGCGGGGCGCGGGAATGCAA  >  NC_000913/1276600‑1276847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑