Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,285,372	A→G	E78E (GAA→GAG)	narJ →	molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,285,372	0	A	G	100.0%	120.5 / NA	33	E78E (GAA→GAG)	narJ	molybdenum‑cofactor‑assembly chaperone delta subunit of nitrate reductase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/20);  total (13/20)

CCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGC  >  NC_000913/1285258‑1285505                                                                                                                   |                                                                                                                                      ccATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGAc                                                                                                               >  2:1327755/1‑139 (MQ=255)       gcTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGc                                                                                                         <  2:945672/139‑1 (MQ=255)            gCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGc                                                                                                    <  1:160359/139‑1 (MQ=255)                    cTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg                                                                                            <  2:1627595/139‑1 (MQ=255)                         cgATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctg                                                                                       <  2:1328506/139‑1 (MQ=255)                            tttAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgctg                                                                                    >  2:79202/1‑139 (MQ=255)                               aaCGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg                                                                                 <  1:1327755/139‑1 (MQ=255)                               aaCGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg                                                                                 <  1:79202/139‑1 (MQ=255)                                      aTGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACg                                                                          >  2:953389/1‑139 (MQ=255)                                      aTGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACg                                                                          >  2:1074105/1‑139 (MQ=255)                                               cTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACTTGCTGGCGCAGTACGAGCAGCACg                                                                 >  2:997934/1‑139 (MQ=255)                                                  gATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCt                                                              <  1:997934/139‑1 (MQ=255)                                                   aTGCCCAGGCTCAGTACAGCGAACTGTTCGACCGGGGCCTCGCCACCTCATTGGTGCTGTTTGAGCCTGTGCAGAGCGAATACCGGGACGGCAGTCTGTCGATGGTGTTCCTGCTGGTGCAGTACGAGCAGCTCGGCtt                                                             <  1:865054/139‑1 (MQ=255)                                                                      cGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGaa                                          <  1:25059/139‑1 (MQ=255)                                                                           tGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCgg                                                                                                          >  2:1760347/1‑70 (MQ=255)                                                                           tGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCgg                                                                                                          <  1:1760347/70‑1 (MQ=255)                                                                             ttCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCgg                                   >  1:1687330/1‑139 (MQ=255)                                                                                gACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGAcc                                <  2:1172011/139‑1 (MQ=255)                                                                                       gCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGcc                         >  2:751082/1‑139 (MQ=255)                                                                                       gCCGCGCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGcc                         <  1:953389/139‑1 (MQ=255)                                                                                         cgcgCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGc                       <  1:882787/139‑1 (MQ=255)                                                                                         cgcgCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGc                       <  1:370068/139‑1 (MQ=255)                                                                                         cgcgCCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGGGGACCGGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACGGCCGCGAATTGCCGGACCATCTGCCGc                       <  1:1074105/139‑1 (MQ=255)                                                                                            gcCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGt                                                                             >  2:187277/1‑82 (MQ=255)                                                                                            gcCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGt                                                                             <  1:187277/82‑1 (MQ=255)                                                                                            gcCACGTCACTGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGt                    >  1:837342/1‑139 (MQ=255)                                                                                                      tGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCt                                                              >  2:1246817/1‑87 (MQ=255)                                                                                                      tGTTGCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCt                                                              <  1:1246817/87‑1 (MQ=255)                                                                                                          gCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTACCGGACCATCTGCCGCTGTATCTGGAGTACCTg      <  2:1467533/139‑1 (MQ=255)                                                                                                          gCTGTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTACCGGACCATCTGCCGCTGTATCTGGAGTACCTg      >  1:1467533/1‑139 (MQ=255)                                                                                                             gTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgc                                                                                  <  1:746104/60‑1 (MQ=255)                                                                                                             gTTTGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgc                                                                                  >  2:746104/1‑60 (MQ=255)                                                                                                              tttGAGCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGgcgc  <  1:529686/139‑1 (MQ=255)                                                                                                                   |                                                                                                                                      CCATGCGCTGGGCATTTTCCTGCGCGATTTAACGACGATGGATCCGCTCGATGCCCAGGCGCAGTACAGCGAACTGTTCGACCGTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAGTACCTGGCGC  >  NC_000913/1285258‑1285505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑