Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,826	C→T	A267T (GCA→ACA)	purU ←	formyltetrahydrofolate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,826	0	C	T	100.0%	189.2 / NA	56	A267T (GCA→ACA)	purU	formyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/30);  total (26/30)

CGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGT  >  NC_000913/1287692‑1287960                                                                                                                                       |                                                                                                                                       cgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  2:1671743/139‑1 (MQ=255) cgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  1:496861/139‑1 (MQ=255) cgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  1:1545308/139‑1 (MQ=255)    tGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACt                                                                                                                                 <  2:1029486/139‑1 (MQ=255)             ttACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGt                                                                                                                        >  2:922275/1‑139 (MQ=255)               aCGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGc                                                                                                                      <  2:698058/139‑1 (MQ=255)                cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa                                                                                                                     >  2:1630625/1‑139 (MQ=255)                     agaAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACt                                                                                                                >  2:1202421/1‑139 (MQ=255)                                     tGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCg                                                                                                >  2:1367418/1‑139 (MQ=255)                                                      tAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcgcg                                                                               >  1:1265747/1‑139 (MQ=255)                                                            aTTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcatcat                                                                         <  1:1367418/139‑1 (MQ=255)                                                               aaaGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATc                                                                      <  1:1202421/139‑1 (MQ=255)                                                                    tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:1240747/139‑1 (MQ=255)                                                                    tAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:1265747/139‑1 (MQ=255)                                                                      aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt                                                               >  2:891684/1‑139 (MQ=255)                                                                      aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCt                                                               >  1:14588/1‑139 (MQ=255)                                                                        cAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGCGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgt                                                             <  2:1018926/139‑1 (MQ=255)                                                                                tttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCtt                                                                    >  1:1765853/1‑124 (MQ=255)                                                                                tttGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCtt                                                                    <  2:1765853/124‑1 (MQ=255)                                                                                     tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTtct                                                                                                  >  1:1528553/1‑89 (MQ=255)                                                                                     tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTtct                                                                                                  <  2:1528553/89‑1 (MQ=255)                                                                                         gATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACAt                                            >  1:1388351/1‑139 (MQ=255)                                                                                                 aataatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  1:216205/110‑1 (MQ=255)                                                                                                 aataatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 >  2:216205/1‑110 (MQ=255)                                                                                                   taatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTc                                  <  2:1388351/139‑1 (MQ=255)                                                                                                     atCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg                                                                                 <  2:515663/90‑1 (MQ=255)                                                                                                     atCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcg                                                                                 >  1:515663/1‑90 (MQ=255)                                                                                                           cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCAc                                                                                         <  1:675520/76‑1 (MQ=255)                                                                                                           cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCAc                                                                                         >  2:675520/1‑76 (MQ=255)                                                                                                           cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTcc                                 <  1:122279/132‑1 (MQ=255)                                                                                                           cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTcc                                 >  2:122279/1‑132 (MQ=255)                                                                                                           cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCat                            >  2:541146/1‑137 (MQ=255)                                                                                                           cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCat                            <  1:541146/137‑1 (MQ=255)                                                                                                             aTTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgt                                                             >  2:1012599/1‑102 (MQ=255)                                                                                                             aTTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgt                                                             <  1:1012599/102‑1 (MQ=255)                                                                                                                aCCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGAtt                     <  2:561241/139‑1 (MQ=255)                                                                                                                  cGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCAc                                                                                         >  2:1271678/1‑69 (MQ=255)                                                                                                                  cGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCAc                                                                                         <  1:1271678/69‑1 (MQ=255)                                                                                                                    tAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGc                 >  1:1375170/1‑139 (MQ=255)                                                                                                                    tAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACATTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGc                 >  2:209344/1‑139 (MQ=255)                                                                                                                      aaCAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCt               >  2:1064196/1‑139 (MQ=255)                                                                                                                           aGACGCGCTGTGTCATTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 >  1:1191951/1‑84 (MQ=255)                                                                                                                           aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:1191951/84‑1 (MQ=255)                                                                                                                           aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 >  1:878763/1‑84 (MQ=255)                                                                                                                           aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTc          <  1:891684/139‑1 (MQ=255)                                                                                                                           aGACGCGCGGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGACACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:878763/84‑1 (MQ=255)                                                                                                                            gACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcgc                                                                                >  2:513501/1‑68 (MQ=255)                                                                                                                            gACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTgcgc                                                                                <  1:513501/68‑1 (MQ=255)                                                                                                                            gACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACAt                                            >  2:1112090/1‑104 (MQ=255)                                                                                                                            gACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGGAGGTATGATCGACAt                                            <  1:1112090/104‑1 (MQ=255)                                                                                                                                cgcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAAt                                        <  1:624516/104‑1 (MQ=255)                                                                                                                                cgcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAAt                                        >  2:624516/1‑104 (MQ=255)                                                                                                                                   tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTc                                                                                           >  2:213927/1‑50 (MQ=255)                                                                                                                                   tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTc                                                                                           <  1:213927/50‑1 (MQ=255)                                                                                                                                   tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt  <  2:747449/139‑1 (MQ=255)                                                                                                                                   tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt  <  1:1064196/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGT  >  NC_000913/1287692‑1287960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑