Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,318,967	G→A	D274D (GAC→GAT)	trpC ←	indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,318,967	0	G	A	100.0%	176.5 / NA	51	D274D (GAC→GAT)	trpC	indole‑3‑glycerolphosphate synthetase and N‑(5‑phosphoribosyl)anthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/29);  total (22/29)

TCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCGTCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGAA  >  NC_000913/1318833‑1319101                                                                                                                                       |                                                                                                                                       tCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGcatcat                                                                                                                                    >  2:1308306/1‑139 (MQ=255)  cGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATa                                                                                                                                   >  2:573992/1‑139 (MQ=255)    gCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAg                                                                                                                                 <  1:573992/139‑1 (MQ=255)          tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTGGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAgc                                                                                                                                <  1:1261285/134‑1 (MQ=255)          tCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAgc                                                                                                                                >  2:1261285/1‑134 (MQ=255)           cGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCttt                                                                                                                          <  2:863883/139‑1 (MQ=255)            gTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTa                                                                                                                         <  2:723307/139‑1 (MQ=255)                   cGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCtt                                                                                                                  >  1:865768/1‑139 (MQ=255)                        cacGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             <  2:128999/139‑1 (MQ=255)                        cacGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             <  2:184782/139‑1 (MQ=255)                                     tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAATAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTCCCCACGCGTCAGGCcac                                                                                                <  1:1022799/139‑1 (MQ=255)                                     tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGcc                                                                                                  >  1:216731/1‑137 (MQ=255)                                     tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGcc                                                                                                  <  2:216731/137‑1 (MQ=255)                                     tGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  2:1331741/139‑1 (MQ=255)                                      gCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcaca                                                                                               >  1:1003882/1‑139 (MQ=255)                                         aCGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATc                                                                                                                    >  1:1287129/1‑115 (MQ=255)                                         aCGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATc                                                                                                                    <  2:1287129/115‑1 (MQ=255)                                         aCGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATAc                                                                                            >  2:260602/1‑139 (MQ=255)                                            gTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACttt                                                                                         >  2:298347/1‑139 (MQ=255)                                            gTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACttt                                                                                         >  2:428835/1‑139 (MQ=255)                                             tGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             >  2:240861/1‑118 (MQ=255)                                             tGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCa                                                                                                             <  1:240861/118‑1 (MQ=255)                                               ccGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTAtt                                                                                      >  2:945914/1‑139 (MQ=255)                                                 gCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTAATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATtct                                                                                    <  1:1388883/139‑1 (MQ=255)                                                    gCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCAc                                                                                 <  1:298347/139‑1 (MQ=255)                                                               cTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACAcc                                                                      <  2:1191306/139‑1 (MQ=255)                                                                    ccTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGgcg                                                                 >  1:730112/1‑139 (MQ=255)                                                                      tGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCa                                                               >  1:930536/1‑139 (MQ=255)                                                                        ttCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTc                                                                                                       >  1:1017259/1‑97 (MQ=255)                                                                        ttCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTc                                                                                                       <  2:1017259/97‑1 (MQ=255)                                                                        ttCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATtc                                                                                     <  1:721952/115‑1 (MQ=255)                                                                        ttCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATtc                                                                                     >  2:721952/1‑115 (MQ=255)                                                                                tGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGccc                                                                                                              <  1:705789/82‑1 (MQ=255)                                                                                tGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGccc                                                                                                              >  2:705789/1‑82 (MQ=255)                                                                                    gCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCaaa                                                 <  1:63178/139‑1 (MQ=255)                                                                                    gCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCaaa                                                 <  1:260602/139‑1 (MQ=255)                                                                                       aCGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCg                                              >  2:959726/1‑139 (MQ=255)                                                                                          cgGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCa                                           <  1:278912/139‑1 (MQ=255)                                                                                           ggTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCAt                                          <  2:930536/139‑1 (MQ=255)                                                                                                      aacaaAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAAc                               >  2:275833/1‑139 (MQ=255)                                                                                                       acaaAAATCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTACAAATCGTCATGGGCCATCAACg                              >  1:1765583/1‑139 (MQ=255)                                                                                                           aaaTCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTACAAATCGTCATGGGCCATCAACGCCGa                          <  2:1765583/139‑1 (MQ=255)                                                                                                            aaTCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGTCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGaa                         <  2:583559/139‑1 (MQ=255)                                                                                                            aaTCAACCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGaa                         <  1:959726/139‑1 (MQ=255)                                                                                                                aaCCCACCGTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCGCCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCaa                     <  1:1308306/139‑1 (MQ=255)                                                                                                                        gTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  2:950530/56‑1 (MQ=255)                                                                                                                        gTAAATCGCGCCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                >  1:950530/1‑56 (MQ=255)                                                                                                                                cgcCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                <  1:254820/48‑1 (MQ=255)                                                                                                                                cgcCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCcac                                                                                                >  2:254820/1‑48 (MQ=255)                                                                                                                                 gcCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTc                                                                                                       <  1:1386073/40‑1 (MQ=255)                                                                                                                                 gcCCGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTc                                                                                                       >  2:1386073/1‑40 (MQ=255)                                                                                                                                   ccGCATCCTAAGCTCCTGTAGCATCTTGCCCACACGTCAGGCCACATACTTTATTCTCCCCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGaa  <  1:290961/139‑1 (MQ=255)                                                                                                                                   ccGCATCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGaa  <  1:275833/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TCGGCAATATCGTGATTGCGGAACACGCCAACATACTGCAACGGTGCCGCAGCCATCACTTCCTGCGCCTGTTCAACGTTGACGCAACGCGGTGATGTCGCAACAAAAATCAACCCACCGTAAATCGCGCCCGCGTCATAAGCTGCTTTAGCATCTTGCCCACGCGTCAGGCCACATACTTTATTCTCACCCAGCAACACCCGGCGCACGGCGGCGTGCAAATCGTCATGGGCCATCAACGCCGAACCAATCAGAAAACCGTTAGCGAA  >  NC_000913/1318833‑1319101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑