Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,399,120 A→G M136T (ATG→ACG)  fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR, autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,399,1200AG100.0% 244.3 / NA 64M136T (ATG→ACG) fnroxygen‑sensing anaerobic growth regulon transcriptional regulator FNR, autorepressor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/26);  total (38/26)

CAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGA  >  NC_000913/1398996‑1399252
                                                                                                                            |                                                                                                                                    
cAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACaaa                                                                                                                        <  2:113618/139‑1 (MQ=255)
             aaTGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGttt                                                                                                           >  2:500224/1‑139 (MQ=255)
             aaTGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGttt                                                                                                           >  2:497358/1‑139 (MQ=255)
             aaTGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGttt                                                                                                           >  2:1594118/1‑139 (MQ=255)
              aTGCAGCCAGACG‑TCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGGCGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCg                                                                                                         >  1:55911/1‑139 (MQ=255)
                      aGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGa                                                                                                  <  1:1594118/139‑1 (MQ=255)
                      aGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGa                                                                                                  <  1:438449/139‑1 (MQ=255)
                            tCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGa                                                                                                  <  2:1774326/133‑1 (MQ=255)
                            tCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGa                                                                                                  >  1:1774326/1‑133 (MQ=255)
                             ccTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       >  2:463921/1‑127 (MQ=255)
                             ccTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       <  1:463921/127‑1 (MQ=255)
                              cTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       >  1:982679/1‑126 (MQ=255)
                              cTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       <  2:982679/126‑1 (MQ=255)
                              cTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACAt                                                                                          >  1:599177/1‑139 (MQ=255)
                                cGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGAc                                                                                                                           >  2:1258929/1‑104 (MQ=255)
                                cGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGAc                                                                                                                           <  1:1258929/104‑1 (MQ=255)
                                cGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATAc                                                                                        >  2:793579/1‑139 (MQ=255)
                                  gCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       >  2:901644/1‑122 (MQ=255)
                                  gCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       <  1:901644/122‑1 (MQ=255)
                                             cGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTcc                                                                           >  2:195479/1‑139 (MQ=255)
                                             cGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATAGAGGTTTcc                                                                           >  1:509091/1‑139 (MQ=255)
                                                          aTCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGa                                                              <  2:599177/139‑1 (MQ=255)
                                                              tGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          >  1:1332277/1‑139 (MQ=255)
                                                              tGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          >  1:784033/1‑139 (MQ=255)
                                                              tGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          >  1:1545316/1‑139 (MQ=255)
                                                              tGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          <  1:497358/139‑1 (MQ=255)
                                                                    gATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTg                                                    >  1:185184/1‑139 (MQ=255)
                                                                    gATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTg                                                    >  2:1679906/1‑139 (MQ=255)
                                                                    gATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTg                                                    >  2:1324932/1‑139 (MQ=255)
                                                                     aTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGa                                                   >  2:349588/1‑139 (MQ=255)
                                                                     aTCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGa                                                   >  2:1171085/1‑139 (MQ=255)
                                                                                tttCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGcc                                        >  2:1768353/1‑139 (MQ=255)
                                                                                    aCCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATg                                    >  2:755785/1‑139 (MQ=255)
                                                                                        cTCATCAGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGt                                >  1:1172361/1‑139 (MQ=255)
                                                                                         tcatcaGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTc                               >  1:1133309/1‑139 (MQ=255)
                                                                                            tcaGACGCATCATCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCaaa                            <  1:1171085/139‑1 (MQ=255)
                                                                                                   catcatCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACggg                                                                                                   <  1:1205235/61‑1 (MQ=255)
                                                                                                   catcatCTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTACGAACggg                                                                                                   >  2:1205235/1‑61 (MQ=255)
                                                                                                     tcatcTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGAttt                                                                                               <  2:518224/63‑1 (MQ=255)
                                                                                                     tcatcTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGAttt                                                                                               >  1:518224/1‑63 (MQ=255)
                                                                                                     tcatcTGCTGACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAg                   >  1:230086/1‑139 (MQ=255)
                                                                                                         ctgctgACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCg               <  1:500224/139‑1 (MQ=255)
                                                                                                          tgctgACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGc              <  1:344384/139‑1 (MQ=255)
                                                                                                          tgctgACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGc              <  2:1332277/139‑1 (MQ=255)
                                                                                                          tgctgACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGc              <  2:230086/139‑1 (MQ=255)
                                                                                                            ctgACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          <  1:273020/93‑1 (MQ=255)
                                                                                                            ctgACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          >  2:273020/1‑93 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       <  1:772839/46‑1 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGaa                                                                                                       >  2:772839/1‑46 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACggg                                                                                                   >  1:1328621/1‑50 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACggg                                                                                                   <  2:1328621/50‑1 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTcaca                                                                                           <  2:1024275/58‑1 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTcaca                                                                                           >  1:1024275/1‑58 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          >  1:958135/1‑91 (MQ=255)
                                                                                                              gACGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCt                                                          <  2:958135/91‑1 (MQ=255)
                                                                                                               aCGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATg                                    >  1:968443/1‑112 (MQ=255)
                                                                                                               aCGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATg                                    <  2:968443/112‑1 (MQ=255)
                                                                                                               aCGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCt         >  2:392920/1‑139 (MQ=255)
                                                                                                                cGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGt                 <  2:350141/130‑1 (MQ=255)
                                                                                                                cGCAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGt                 >  1:350141/1‑130 (MQ=255)
                                                                                                                  cAGATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTaaa      >  1:793640/1‑139 (MQ=255)
                                                                                                                    gATTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATg    <  1:301261/139‑1 (MQ=255)
                                                                                                                     aTTCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATgg   >  2:727970/1‑139 (MQ=255)
                                                                                                                      ttCGGCGTTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGa  <  1:392920/139‑1 (MQ=255)
                                                                                                                            |                                                                                                                                    
CAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCATTTTACCGGACAAATCGTCCAGCGTTTCGAACGGGATTTCACATACCATCGAGGTTTCCAGCGCCTGCGCGAAGCTCGGGTGATGGCCGCTGCCGATGGCGTCAAATCCCACCAGGTCGCCTGCTAAATGGA  >  NC_000913/1398996‑1399252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑