Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,343	A→G	Y167C (TAC→TGC)	purR →	transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,343	0	A	G	100.0%	175.0 / NA	46	Y167C (TAC→TGC)	purR	transcriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/20);  total (26/20)

TGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGT  >  NC_000913/1738215‑1738475                                                                                                                                 |                                                                                                                                     tGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCggg                                                                                                                            <  1:603408/139‑1 (MQ=255)                 ttGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAAc                                                                                                            >  2:940376/1‑139 (MQ=255)                                gAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                      >  2:1055789/1‑114 (MQ=255)                                gAAGAGTATCGCCATATCCCAATGGTGGTCATAGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                      <  1:1055789/114‑1 (MQ=255)                                                    aaTGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCg                                                                         >  2:1452397/1‑139 (MQ=255)                                                          ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                                   >  1:265582/1‑139 (MQ=255)                                                           gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTGATTGATAACGCGTTCGAAGGCGGCTGCATGGCCgggt                                                                                                                           <  1:1194775/82‑2 (MQ=255)                                                           gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAAc                                                                  >  2:1713486/1‑139 (MQ=255)                                                           gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATAGCCgggt                                                                                                                           >  2:1194775/1‑81 (MQ=255)                                                               tGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTaa                                                              <  2:26873/139‑1 (MQ=255)                                                                  aCTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTTacac                                                           <  1:1038271/139‑1 (MQ=255)                                                                      gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      >  2:941014/1‑108 (MQ=255)                                                                      gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      <  1:941014/108‑1 (MQ=255)                                                                      gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGgc                                                       >  1:221978/1‑139 (MQ=255)                                                                            aGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCAGCGCAGgc                                                 >  2:1187672/1‑139 (MQ=255)                                                                                aaaGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      <  1:179036/98‑1 (MQ=255)                                                                                aaaGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      >  2:179036/1‑98 (MQ=255)                                                                                aaaGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGgccgcc                                             >  1:57171/1‑139 (MQ=255)                                                                                       aCTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGc                                         <  2:707835/136‑1 (MQ=255)                                                                                       aCTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGc                                         >  1:707835/1‑136 (MQ=255)                                                                                        cTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                          >  1:110975/1‑54 (MQ=255)                                                                                        cTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                          <  2:110975/54‑1 (MQ=255)                                                                                        cTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGt                                     >  1:1162550/1‑139 (MQ=255)                                                                                              cGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATg                               <  2:221978/139‑1 (MQ=255)                                                                                                 tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGt                                     >  2:1458711/1‑130 (MQ=255)                                                                                                 tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGt                                     <  1:1458711/130‑1 (MQ=255)                                                                                                 tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg                            <  2:57171/139‑1 (MQ=255)                                                                                                 tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg                            >  1:451829/1‑139 (MQ=255)                                                                                                  gCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCACCTTGCCGGTTTTATGAAgg                           <  1:1713486/139‑1 (MQ=255)                                                                                                   cGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCgg                                      >  1:740623/1‑127 (MQ=255)                                                                                                   cGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCgg                                      <  2:740623/127‑1 (MQ=255)                                                                                                      tCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGAt                       <  2:265582/139‑1 (MQ=255)                                                                                                        aTTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCg                                                                              >  1:1558864/1‑82 (MQ=255)                                                                                                        aTTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCg                                                                              <  2:1558864/82‑1 (MQ=255)                                                                                                                cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGa                              >  1:63341/1‑122 (MQ=255)                                                                                                                cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGa                              <  2:63341/122‑1 (MQ=255)                                                                                                                cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaagaa                <  1:152359/136‑1 (MQ=255)                                                                                                                cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaagaa                >  2:152359/1‑136 (MQ=255)                                                                                                                cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCg             >  1:783558/1‑139 (MQ=255)                                                                                                                cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCg             >  1:1757183/1‑139 (MQ=255)                                                                                                                    ttCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaag                  <  2:757522/130‑1 (MQ=255)                                                                                                                    ttCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaag                  >  1:757522/1‑130 (MQ=255)                                                                                                                    ttCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCgatga         >  1:595162/1‑139 (MQ=255)                                                                                                                           gcggcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCGCCg                                                                              >  1:1178782/1‑63 (MQ=255)                                                                                                                           gcggcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCg                                                                              <  2:1178782/63‑1 (MQ=255)                                                                                                                           gcggcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGt  >  1:136856/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     TGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGT  >  NC_000913/1738215‑1738475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑