Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,102	G→A	A45V (GCA→GTA)	yoaA ←	putative ATP‑dependent helicase, DinG family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,102	0	G	A	100.0%	99.1 / NA	30	A45V (GCA→GTA)	yoaA	putative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/16);  total (14/16)

TTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAA  >  NC_000913/1892971‑1893224                                                                                                                                    |                                                                                                                           ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccac                                                                                                                     >  1:680889/1‑139 (MQ=255) ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTccac                                                                                                                     >  1:1485167/1‑139 (MQ=255)        tGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGc                                                                                                              >  1:326131/1‑139 (MQ=255)           tGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGc                                                                                                           <  2:680889/139‑1 (MQ=255)           tGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGc                                                                                                           <  1:1760130/139‑1 (MQ=255)            ggCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCt                                                                                                          >  2:57080/1‑139 (MQ=255)            ggCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCt                                                                                                          >  2:1739490/1‑139 (MQ=255)                        cTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTa                                                                                              <  1:1383963/139‑1 (MQ=255)                         tGTAGGGCTGATCCTGCTACGCTTTTGAGCCGGTCGAGATAAGGACTTTCTTTTTCGACCGCAGCGCAGGAGCCAGGTAAGCGTAGTTTTTGCCCGTACCGGTTCCTACTTCCACCACCTGCGGCTGGCCTTTTTCTAt                                                                                             <  1:824502/139‑1 (MQ=255)                             gagCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGcc                                                                                         <  1:1698172/139‑1 (MQ=255)                                   aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCt                                                                                        >  1:1518655/1‑134 (MQ=255)                                   aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCt                                                                                        <  2:1518655/134‑1 (MQ=255)                                   aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg                                                                                   <  1:1739490/139‑1 (MQ=255)                                   aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg                                                                                   >  2:1417161/1‑139 (MQ=255)                                   aTCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg                                                                                   <  2:1043498/139‑1 (MQ=255)                                                       cGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCg                                                               <  1:1417161/139‑1 (MQ=255)                                                                   tGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcg                                                   <  2:1145736/139‑1 (MQ=255)                                                                   tGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcg                                                   >  1:1145736/1‑139 (MQ=255)                                                                    gACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTcgc                                                  <  1:1012035/139‑1 (MQ=255)                                                                         tctttTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCtt                                             >  2:1505615/1‑139 (MQ=255)                                                                          ctttTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTATATCGCATGGGTGACGGCTACCACCATCTGTCGCTGTGGTTCTCGCGGCTTa                                            <  1:1032979/139‑1 (MQ=255)                                                                                                   aGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCtgt                                                           >  2:1062263/1‑99 (MQ=255)                                                                                                   aGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCtgt                                                           <  1:1062263/99‑1 (MQ=255)                                                                                                   aGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCt                   >  1:1774349/1‑139 (MQ=255)                                                                                                       aaGCGTAGGTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGAcc               <  2:326131/139‑1 (MQ=255)                                                                                                              ggTTTTGCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGt        >  2:1510546/1‑139 (MQ=255)                                                                                                                    gCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCaaaa  <  2:1774349/139‑1 (MQ=255)                                                                                                                    gCCCGTACCGGTTCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCaaaa  >  1:1345532/1‑139 (MQ=255)                                                                                                                               ttCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCGTCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTa                                  <  1:330378/96‑1 (MQ=255)                                                                                                                               ttCCTACTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTa                                  >  2:330378/1‑96 (MQ=255)                                                                                                                                    |                                                                                                                           TTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTACCGCCATCTGTCGCTGTGGTTCTCGCGGCTTAAAGCCTGGTATCGCTTTCGCCAGCTGACCGTCTGGTGCAAAA  >  NC_000913/1892971‑1893224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑