Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,904,586	T→C	V231A (GTC→GCC)	manZ →	mannose‑specific enzyme IID component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,586	0	T	C	100.0%	245.0 / NA	65	V231A (GTC→GCC)	manZ	mannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/35);  total (30/35)

AAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTA  >  NC_000913/1904453‑1904721                                                                                                                                      |                                                                                                                                        aaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGa                                                                                                                                    <  1:878682/139‑1 (MQ=255) aaaCTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGa                                                                                                                                    <  2:388030/139‑1 (MQ=255)            aGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         <  1:1083792/139‑1 (MQ=255)            aGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         <  1:1510120/139‑1 (MQ=255)                     tATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATg                                                                                                                >  2:1179279/1‑139 (MQ=255)                       tCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGcc                                                                                                              <  1:1179279/139‑1 (MQ=255)                               cTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTga                                                                                                      <  2:1039883/139‑2 (MQ=255)                                    tGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCa                                                                                                 <  1:927057/139‑1 (MQ=255)                                     gTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTATGGACCAGTTAATGCCAGGCCTGATACCAc                                                                                                <  1:101777/139‑1 (MQ=255)                                                     ttAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                <  2:1308586/139‑1 (MQ=255)                                                       aaCAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCtt                                                                              >  2:1312139/1‑139 (MQ=255)                                                        aCAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTg                                                                             <  1:1312139/139‑1 (MQ=255)                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGcc                                                                                                              <  2:206405/100‑1 (MQ=255)                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGcc                                                                                                              >  1:206405/1‑100 (MQ=255)                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                       >  2:596011/1‑139 (MQ=255)                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                       >  2:194457/1‑139 (MQ=255)                                                              ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                       >  2:700559/1‑139 (MQ=255)                                                               gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                <  1:1442518/129‑1 (MQ=255)                                                               gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                >  2:1442518/1‑129 (MQ=255)                                                                    aTGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGcc                                                                                                         <  1:917410/99‑1 (MQ=255)                                                                    aTGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGcc                                                                                                         >  2:917410/1‑99 (MQ=255)                                                                      gTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTgc                                                               >  2:563945/1‑139 (MQ=255)                                                                      gTCAACATCCCACTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTgc                                                               >  1:818001/1‑139 (MQ=255)                                                                              cccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         <  2:601082/73‑1 (MQ=255)                                                                              cccGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         >  1:601082/1‑73 (MQ=255)                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCa                                                                                                             <  2:974284/83‑1 (MQ=255)                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCa                                                                                                             >  1:974284/1‑83 (MQ=255)                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                <  2:428053/112‑1 (MQ=255)                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                >  1:428053/1‑112 (MQ=255)                                                                                cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGt                                                     >  2:1053868/1‑139 (MQ=255)                                                                                  cTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTa                                                   >  1:1044372/1‑139 (MQ=255)                                                                                     gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCAc                                                                                                <  2:112619/91‑1 (MQ=255)                                                                                     gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCAc                                                                                                >  1:112619/1‑91 (MQ=255)                                                                                     gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAcc                                                <  1:596011/139‑1 (MQ=255)                                                                                     gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAcc                                                <  2:1044372/139‑1 (MQ=255)                                                                                        gTCTCTCGCATTACTGACCAGCCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGc                                             >  2:376861/1‑139 (MQ=255)                                                                                        gTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCa                                                                                                 >  2:1268308/1‑87 (MQ=255)                                                                                        gTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCa                                                                                                 <  1:1268308/87‑1 (MQ=255)                                                                                         tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCt                                            <  1:1118214/139‑1 (MQ=255)                                                                                         tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCt                                            <  2:818001/139‑1 (MQ=255)                                                                                           tctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCtgt                                          >  1:41284/1‑139 (MQ=255)                                                                                              cGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGa                                       <  1:400058/139‑1 (MQ=255)                                                                                                   tACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                              >  2:1631646/1‑127 (MQ=255)                                                                                                   tACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                              <  1:1631646/127‑1 (MQ=255)                                                                                                        aCCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         >  2:1778419/1‑47 (MQ=255)                                                                                                        aCCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGAc                                                                                                                         <  1:1778419/47‑1 (MQ=255)                                                                                                         ccAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGc                            <  1:563945/139‑1 (MQ=255)                                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                >  2:1306743/1‑83 (MQ=255)                                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                <  1:1306743/83‑1 (MQ=255)                                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                 <  2:1314298/114‑1 (MQ=255)                                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                 >  1:1314298/1‑114 (MQ=255)                                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGcttct                        >  2:1709945/1‑139 (MQ=255)                                                                                                             aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGcttct                        >  2:441420/1‑139 (MQ=255)                                                                                                               gggCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGcc                                                                                                         >  2:1623515/1‑56 (MQ=255)                                                                                                               gggCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGcc                                                                                                         <  1:1623515/56‑1 (MQ=255)                                                                                                                  cAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                 >  1:134759/1‑109 (MQ=255)                                                                                                                  cAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                 <  2:134759/109‑1 (MQ=255)                                                                                                                    aaGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCAt                 <  1:194457/139‑1 (MQ=255)                                                                                                                           cGTTGCTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                 <  2:631587/100‑1 (MQ=255)                                                                                                                           cGTTGCTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                 >  1:631587/1‑100 (MQ=255)                                                                                                                           cGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAgg                                                                                                           <  1:663560/42‑1 (MQ=255)                                                                                                                           cGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAgg                                                                                                           >  2:663560/1‑42 (MQ=255)                                                                                                                           cGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATc          >  1:600807/1‑139 (MQ=255)                                                                                                                           cGTTACTACTGCCCAGACTAGTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATc          <  2:600807/139‑1 (MQ=255)                                                                                                                                   ctGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTa  <  1:1053868/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AAACTGACGGAAGGGGCGTCTATCCTCGGCCTGTTTGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTA  >  NC_000913/1904453‑1904721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑