Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,904,621 G→A V243I (GTA→ATA)  manZ → mannose‑specific enzyme IID component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,904,6210GA100.0% 279.7 / NA 81V243I (GTA→ATA) manZmannose‑specific enzyme IID component of PTS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/37);  total (44/37)

TGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTG  >  NC_000913/1904488‑1904754
                                                                                                                                     |                                                                                                                                     
tGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCa                                                                                                                                  <  1:927057/139‑1 (MQ=255)
 gTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTATGGACCAGTTAATGCCAGGCCTGATACCAc                                                                                                                                 <  1:101777/139‑1 (MQ=255)
                 ttAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 <  2:1308586/139‑1 (MQ=255)
                   aaCAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCtt                                                                                                               >  2:1312139/1‑139 (MQ=255)
                    aCAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTg                                                                                                              <  1:1312139/139‑1 (MQ=255)
                          ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                                                        >  2:700559/1‑139 (MQ=255)
                          ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                                                        >  2:596011/1‑139 (MQ=255)
                          ggACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTAtgtg                                                                                                        >  2:194457/1‑139 (MQ=255)
                           gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 <  1:1442518/129‑1 (MQ=255)
                           gACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 >  2:1442518/1‑129 (MQ=255)
                                  gTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTgc                                                                                                >  2:563945/1‑139 (MQ=255)
                                  gTCAACATCCCACTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTgc                                                                                                >  1:818001/1‑139 (MQ=255)
                                            cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 >  1:428053/1‑112 (MQ=255)
                                            cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 <  2:428053/112‑1 (MQ=255)
                                            cGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGt                                                                                      >  2:1053868/1‑139 (MQ=255)
                                              cTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTa                                                                                    >  1:1044372/1‑139 (MQ=255)
                                                 gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCAc                                                                                                                                 <  2:112619/91‑1 (MQ=255)
                                                 gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCAc                                                                                                                                 >  1:112619/1‑91 (MQ=255)
                                                 gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAcc                                                                                 <  2:1044372/139‑1 (MQ=255)
                                                 gTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAcc                                                                                 <  1:596011/139‑1 (MQ=255)
                                                    gTCTCTCGCATTACTGACCAGCCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGc                                                                              >  2:376861/1‑139 (MQ=255)
                                                    gTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCa                                                                                                                                  <  1:1268308/87‑1 (MQ=255)
                                                    gTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCa                                                                                                                                  >  2:1268308/1‑87 (MQ=255)
                                                     tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCt                                                                             <  2:818001/139‑1 (MQ=255)
                                                     tctctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCt                                                                             <  1:1118214/139‑1 (MQ=255)
                                                       tctcGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCtgt                                                                           >  1:41284/1‑139 (MQ=255)
                                                          cGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGa                                                                        <  1:400058/139‑1 (MQ=255)
                                                               tACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                                                               <  1:1631646/127‑1 (MQ=255)
                                                               tACTGACCAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                                                               >  2:1631646/1‑127 (MQ=255)
                                                                     ccAGACGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGc                                                             <  1:563945/139‑1 (MQ=255)
                                                                         aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 >  2:1306743/1‑83 (MQ=255)
                                                                         aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGc                                                                                                                 <  1:1306743/83‑1 (MQ=255)
                                                                         aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  <  2:1314298/114‑1 (MQ=255)
                                                                         aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  >  1:1314298/1‑114 (MQ=255)
                                                                         aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGcttct                                                         >  2:441420/1‑139 (MQ=255)
                                                                         aCGGGCAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGcttct                                                         >  2:1709945/1‑139 (MQ=255)
                                                                              cAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  >  1:134759/1‑109 (MQ=255)
                                                                              cAAAGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  <  2:134759/109‑1 (MQ=255)
                                                                                aaGAACACGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCAt                                                  <  1:194457/139‑1 (MQ=255)
                                                                                       cGTTGCTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  >  1:631587/1‑100 (MQ=255)
                                                                                       cGTTGCTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  <  2:631587/100‑1 (MQ=255)
                                                                                       cGTTACTACTGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATc                                           >  1:600807/1‑139 (MQ=255)
                                                                                       cGTTACTACTGCCCAGACTAGTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATc                                           <  2:600807/139‑1 (MQ=255)
                                                                                               ctGCCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTa                                   <  1:1053868/139‑1 (MQ=255)
                                                                                                tgcccAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt                                     >  2:1687177/4‑136 (MQ=255)
                                                                                                tgcccAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt                                     <  1:1687177/133‑1 (MQ=255)
                                                                                                 gcccAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACg                                 >  1:1181536/3‑139 (MQ=255)
                                                                                                  cccAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGc                                >  1:423232/2‑139 (MQ=255)
                                                                                                    cAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCtt                              >  2:1785106/1‑139 (MQ=255)
                                                                                                    cAGACTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCtt                              >  2:1003729/1‑139 (MQ=255)
                                                                                                       aCTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCg                           >  1:1178097/1‑139 (MQ=255)
                                                                                                        cTATTCTGGACCAGTTATTGCCAGGCCTGCTATCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTACCCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCgg                          <  1:376861/139‑1 (MQ=255)
                                                                                                        cTATTCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCgg                          <  1:1785106/139‑1 (MQ=255)
                                                                                                            tCTGGACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCctg                      >  1:349847/1‑139 (MQ=255)
                                                                                                                gACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                                                               >  1:175377/1‑78 (MQ=255)
                                                                                                                gACCAGTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCg                                                                               <  2:175377/78‑1 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  >  1:356091/1‑70 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAAc                                                                                  <  2:356091/70‑1 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatca                                                                     >  2:687585/1‑83 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGatca                                                                     <  1:687585/83‑1 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt                                     >  2:1219519/1‑115 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGt                                     <  1:1219519/115‑1 (MQ=255)
                                                                                                                     gTTAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTg             >  1:306941/1‑139 (MQ=255)
                                                                                                                      ttAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTg                                                                                                              >  1:1437726/1‑41 (MQ=255)
                                                                                                                      ttAATGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTg                                                                                                              <  2:1437726/41‑1 (MQ=255)
                                                                                                                        aaTGCCAGGCCTGATATCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTaa          <  1:1705926/139‑1 (MQ=255)
                                                                                                                        aaTGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTaa          <  1:700559/139‑1 (MQ=255)
                                                                                                                          tGCCAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGa        <  1:441420/139‑1 (MQ=255)
                                                                                                                             cAGGCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtg     <  2:423232/139‑1 (MQ=255)
                                                                                                                               ggCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTggg                 <  1:337698/125‑1 (MQ=255)
                                                                                                                               ggCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTggg                 >  2:337698/1‑125 (MQ=255)
                                                                                                                               ggCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtgtt   >  1:970394/1‑139 (MQ=255)
                                                                                                                               ggCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtgtt   >  1:542680/1‑139 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCaa                                                                                            >  1:1157984/1‑49 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCaa                                                                                            <  2:1157984/49‑1 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTAt                                            >  1:265494/1‑97 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTAt                                            <  2:265494/97‑1 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCg                           <  2:737840/114‑1 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCg                           >  1:737840/1‑114 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtgttg  >  2:43328/1‑139 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtgttg  >  1:417765/1‑139 (MQ=255)
                                                                                                                                gCCTGATACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACtgttg  >  2:1619481/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
TGTCATGGGGGCATTGGTTAACAAGTGGACACATGTCAACATCCCGCTGGTTGTCTCTCGCATTACTGACCAGACGGGCAAAGAACACGTTACTACTGTCCAGACTATTCTGGACCAGTTAATGCCAGGCCTGGTACCACTGCTGCTGACCTTTGCTTGTATGTGGCTACTGCGCAAAAAAGTTAACCCGCTGTGGATCATCGTTGGCTTCTTCGTCATCGGTATCGCTGGTTACGCTTGCGGCCTGCTGGGACTGTAAGACTGTTG  >  NC_000913/1904488‑1904754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑