Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,262,371	G→A	G375G (GGC→GGT)	fruB ←	fused fructose‑specific PTS enzymes: IIA component/HPr component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,371	0	G	A	100.0%	127.6 / NA	38	G375G (GGC→GGT)	fruB	fused fructose‑specific PTS enzymes: IIA component/HPr component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/17);  total (21/17)

TGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACG  >  NC_000913/2262236‑2262502                                                                                                                                        |                                                                                                                                    tGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCAccc                                                                                                                                  <  1:28879/139‑1 (MQ=255)                               tggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCg                                                                                                    >  2:11441/1‑139 (MQ=255)                               tggtTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCg                                                                                                    >  1:200135/1‑139 (MQ=255)                                      cACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCtt                                                                                             >  1:717381/1‑139 (MQ=255)                                           ggTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAgc                                                                                        <  2:985861/139‑1 (MQ=255)                                            gTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAgcg                                                                                       >  1:182330/1‑139 (MQ=255)                                             ttCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAgcgc                                                                                      <  2:717381/139‑1 (MQ=255)                                                aCTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTg                                                                                   <  1:11441/139‑1 (MQ=255)                                                  ttCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGt                                                                                  >  2:1765418/1‑138 (MQ=255)                                                  ttCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGt                                                                                  <  1:1765418/138‑1 (MQ=255)                                                     gccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAg                                                                              <  1:948720/139‑1 (MQ=255)                                                     gccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAg                                                                              <  1:1614918/139‑1 (MQ=255)                                                      ccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTc                                                                                <  1:187/136‑1 (MQ=255)                                                      ccgcGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTc                                                                                >  2:187/1‑136 (MQ=255)                                                                        cAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGt                                                           >  2:5453/1‑139 (MQ=255)                                                                                aacaaGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTa                                                   <  1:1294618/139‑1 (MQ=255)                                                                                       tCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATg                                            >  2:577843/1‑139 (MQ=255)                                                                                         aTAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGAc                                          >  2:478172/1‑139 (MQ=255)                                                                                         aTAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGAc                                          <  1:1516110/139‑1 (MQ=255)                                                                                                 gATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTa                                  >  1:1015521/1‑139 (MQ=255)                                                                                                         gTGATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAg                          <  1:577843/139‑1 (MQ=255)                                                                                                           gATAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTg                        >  1:512556/1‑139 (MQ=255)                                                                                                            aTAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACca                                                                                                                      <  2:344938/44‑1 (MQ=255)                                                                                                            aTAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACca                                                                                                                      >  1:344938/1‑44 (MQ=255)                                                                                                            aTAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGc                       >  1:684545/1‑139 (MQ=255)                                                                                                            aTAGTAGCAACACGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGc                       >  1:80574/1‑139 (MQ=255)                                                                                                                     acacGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACttt              >  1:1564648/1‑139 (MQ=255)                                                                                                                        cGTCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcat           <  2:200135/139‑1 (MQ=255)                                                                                                                          tCTGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTtcatca         >  2:1720329/1‑139 (MQ=255)                                                                                                                            tGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAg                                                                              >  2:1177123/1‑68 (MQ=255)                                                                                                                            tGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAg                                                                              <  1:1177123/68‑1 (MQ=255)                                                                                                                            tGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTaaa                                                        <  2:1041133/90‑1 (MQ=255)                                                                                                                            tGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTaaa                                                        >  1:1041133/1‑90 (MQ=255)                                                                                                                            tGCTCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGa       >  1:1265551/1‑139 (MQ=255)                                                                                                                               tCATTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTa    <  2:182330/139‑1 (MQ=255)                                                                                                                                 aTTATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACg  >  1:342582/1‑139 (MQ=255)                                                                                                                                   tATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTc                                                                                           <  2:1559793/48‑1 (MQ=255)                                                                                                                                   tATGCACCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTc                                                                                           >  1:1559793/1‑48 (MQ=255)                                                                                                                                        |                                                                                                                                    TGATGCCTTTACCCGCCGCATGCAGACCGGTGGTTTTCACCAGGTTCACTTCGCCGCGTTCAATTTCCGGGCAGAAACCAACAAGGTCATAAGCCGGATTAAGGGTGATAGTAGCAACACGTCTGCTCATTATGCGCCCTCCCCAAGACCAGCAGCGATAGCGTCGCCGATTGCTTTCAGCGCCTGTTCAGCATCTGCACCCTGGGCGGTAAAGCGTAGGCGATGACCTTTCTTAACGCCAAGTGCCACAACTTTCATCAGACTACG  >  NC_000913/2262236‑2262502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑