Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,288,554 | G→C | pseudogene (361/2525 nt) | yejO ← | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,288,554 | 0 | G | C | 94.2% | 233.9 / 7.4 | 69 | pseudogene (361/2525 nt) | yejO | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
Reads supporting (aligned to +/- strand): ref base G (2/2); new base C (35/30); total (37/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.01e-01 |
GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG > NC_000913/2288420‑2288688 | gaCGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑Ccccc > 2:470611/1‑139 (MQ=255) ggTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑cccccccc < 1:470611/139‑1 (MQ=255) aTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAAc < 2:111034/139‑1 (MQ=255) ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAgcgc < 1:1054813/139‑1 (MQ=255) ggCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAgcgc > 2:1479661/1‑139 (MQ=255) cgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCt > 1:1491237/1‑139 (MQ=255) gTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCtt > 1:453344/1‑139 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑cccccc < 1:1320280/91‑1 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑cccccc > 2:1320280/1‑91 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGcgcc < 2:1261472/116‑1 (MQ=255) aCCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTGAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGcgcc > 1:1261472/1‑116 (MQ=255) ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCttt > 2:603294/1‑122 (MQ=255) ccGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCttt < 1:603294/122‑1 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 2:1023210/1‑139 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 1:572998/1‑139 (MQ=255) ttCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt < 2:453344/139‑1 (MQ=255) cTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaa < 2:572998/139‑1 (MQ=255) aTCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACGCCCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATaaaa > 2:1611730/1‑138 (MQ=255) tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGt > 2:1336685/1‑139 (MQ=255) ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg < 2:1479769/139‑1 (MQ=255) ccGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg > 1:1553094/1‑139 (MQ=255) cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGc > 2:325994/1‑139 (MQ=255) caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGGCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGcac > 1:1157952/1‑139 (MQ=255) aatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCacac < 1:325994/139‑1 (MQ=255) cccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc < 1:1738139/130‑1 (MQ=255) cccccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACAcc > 2:1738139/1‑130 (MQ=255) ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGt > 1:1295588/1‑139 (MQ=255) ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGt > 1:222805/1‑139 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg < 2:457764/108‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg > 1:457764/1‑108 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa > 2:422767/1‑115 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa < 1:422767/115‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGcc > 1:179154/1‑139 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAg > 2:1168435/1‑60 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAg < 1:1168435/60‑1 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg > 2:69557/1‑107 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg < 1:69557/107‑1 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcg > 1:360018/1‑139 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑‑GCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa < 2:226025/72‑1 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGAC‑‑GCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTaa > 1:226025/1‑72 (MQ=255) tcctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGcgccc < 1:1331744/60‑1 (MQ=255) tcctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGcgccc > 2:1331744/1‑60 (MQ=255) tcctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCa < 2:1724885/139‑1 (MQ=255) ccctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg < 2:24120/70‑1 (MQ=255) ccctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCg > 1:24120/2‑71 (MQ=255) cctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc > 2:133578/1‑139 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAgcgc < 2:299515/56‑1 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAgcgc > 1:299515/1‑56 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt > 2:288841/1‑90 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt < 1:288841/90‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTACGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 2:862623/1‑82 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAgcgc < 2:1782114/54‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAgcgc > 1:1782114/1‑54 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGcgcc < 2:202366/55‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGcgcc > 1:202366/1‑55 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg < 1:862623/82‑1 (MQ=255) cccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCa < 1:133578/139‑1 (MQ=255) cccTTTTCGATAGCGACAAAGAC‑‑GCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa > 1:132235/1‑104 (MQ=255) cccTTTTCGATAGCGACAAAGAC‑‑GCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCa < 2:132235/104‑1 (MQ=255) ccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCATCGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg < 1:1179723/81‑1 (MQ=255) ccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 2:1179723/1‑81 (MQ=255) ccTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAt < 2:1553094/139‑1 (MQ=255) cTTTTCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCAtt > 2:1019635/1‑139 (MQ=255) tttCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGGGCGTAGTCTGGTTTGCCGCGCCACCCATTTg < 1:1336685/139‑1 (MQ=255) tCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg > 2:382165/1‑76 (MQ=255) tCGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTg < 1:382165/76‑1 (MQ=255) tCGATAGCGACAAAGACG‑CCCCCCCCAGCTTTTTCAACGATTAAGcgccc < 1:534712/50‑1 (MQ=255) tCGATAGCGACAAAGACG‑CACCCCCCATCTTTTTCAACGATTAAGcgccc > 2:534712/1‑50 (MQ=255) cGATAGCGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAAc < 1:166198/139‑1 (MQ=255) cGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt > 1:1045363/1‑52 (MQ=255) cGACAAAGACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt < 2:1045363/52‑1 (MQ=255) gACG‑CCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCg < 2:1429798/139‑1 (MQ=255) | GACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCCCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGATAACCG > NC_000913/2288420‑2288688 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |