Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,465,279	T→C	intergenic (‑272/‑22)	mlaA ← / → yfdC	ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,465,279	0	T	C	100.0%	257.8 / NA	68	intergenic (‑272/‑22)	mlaA/yfdC	ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/35);  total (33/35)

CATAGCCTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCC  >  NC_000913/2465144‑2465405                                                                                                                                        |                                                                                                                               cATAGCCTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCt                                                                                                                             <  2:1285128/139‑1 (MQ=255) cATAGCCTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCt                                                                                                                             >  2:1359716/1‑139 (MQ=255)      ccTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTc                                                                                                                        >  2:1219834/1‑139 (MQ=255)      ccTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTc                                                                                                                        >  2:820308/1‑139 (MQ=255)      ccTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGC‑‑TACGCGCTAATTCaa                                                                                                                      >  1:145781/1‑139 (MQ=255)         ggTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTAGATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaag                                                                                                                     >  1:1044029/1‑139 (MQ=255)         ggTAATCGCCG‑CCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaaga                                                                                                                    >  2:1565493/1‑139 (MQ=255)          gTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaaga                                                                                                                    >  2:1642704/1‑139 (MQ=255)              tCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaag                                                                                                                <  1:1207435/139‑1 (MQ=255)              tCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaag                                                                                                                <  2:1082157/139‑1 (MQ=255)              tCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaag                                                                                                                <  1:1359716/139‑1 (MQ=255)              tCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaag                                                                                                                <  1:820308/139‑1 (MQ=255)                gccgccCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaagag                                                                                                              >  2:1053439/1‑139 (MQ=255)                   gccCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGAcc                                                                                                           <  2:746870/139‑1 (MQ=255)                         tAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGAc                                                                                                     >  1:713019/1‑139 (MQ=255)                           ggCTACTTGATTCCATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGATCATAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACaa                                                                                                   <  1:1180379/139‑1 (MQ=255)                               aCTTGATTCTTTAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGAc                                                                                               >  1:685254/1‑139 (MQ=255)                                cTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACa                                                                                              >  1:358179/1‑139 (MQ=255)                                 ttGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaa                                                                                             >  1:131331/1‑139 (MQ=255)                                   gATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaaaa                                                                                           >  1:1598702/1‑139 (MQ=255)                                   gATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaaaa                                                                                           <  2:1259319/139‑1 (MQ=255)                                   gATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaaaa                                                                                           >  1:625490/1‑139 (MQ=255)                                   gATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaaaa                                                                                           <  1:1053439/139‑1 (MQ=255)                                        tataGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaa                                                                                             >  1:1646169/1‑132 (MQ=255)                                        tataGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaa                                                                                             <  2:1646169/132‑1 (MQ=255)                                        tataGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGAt                                                                                      <  1:1206511/139‑1 (MQ=255)                                          taGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCa                                                                                    >  1:618887/1‑139 (MQ=255)                                             aaaCAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAaca                                                                                 <  2:586217/139‑1 (MQ=255)                                               aCAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAAcaca                                                                               >  1:1170988/1‑139 (MQ=255)                                                   aaaaaGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGa                                                                                                <  2:1237685/118‑1 (MQ=255)                                                   aaaaaGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGa                                                                                                >  1:1237685/1‑118 (MQ=255)                                                      aaGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGcgacga                                                                        <  2:618887/139‑1 (MQ=255)                                                        ggCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGaaa                                                                      <  1:1642704/139‑1 (MQ=255)                                                             aCGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGaa                                                                 >  2:188905/1‑139 (MQ=255)                                                                     ttttCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGagag                                                         <  2:131331/139‑1 (MQ=255)                                                                       ttCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                                       <  2:124972/139‑1 (MQ=255)                                                                       ttCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                                       <  2:1598702/139‑1 (MQ=255)                                                                       ttCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                                       <  2:1044029/139‑1 (MQ=255)                                                                       ttCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                                       <  1:155964/139‑1 (MQ=255)                                                                        tCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACATAATTGATCAACACAGcga                                                                           <  1:992866/118‑1 (MQ=255)                                                                        tCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGcga                                                                           >  2:992866/1‑118 (MQ=255)                                                                        tCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCga                                                      >  1:1730309/1‑139 (MQ=255)                                                                             aTTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGcgacga                                                                        >  2:867605/1‑116 (MQ=255)                                                                             aTTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGcgacga                                                                        <  1:867605/116‑1 (MQ=255)                                                                                         aCTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCa                                     <  1:1131317/139‑1 (MQ=255)                                                                                          cTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaaaa                                                                                           <  2:1711102/84‑1 (MQ=255)                                                                                          cTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACaaaa                                                                                           >  1:1711102/1‑84 (MQ=255)                                                                                          cTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCaa                                    >  1:868892/1‑139 (MQ=255)                                                                                               gTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCa                                                                                                          <  2:1382355/64‑1 (MQ=255)                                                                                               gTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCa                                                                                                          >  1:1382355/1‑64 (MQ=255)                                                                                               gTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCaaaaaaa                               >  2:808701/1‑139 (MQ=255)                                                                                                   gTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaataga                           <  2:1730309/139‑1 (MQ=255)                                                                                                    ttGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaaga                                                                                                               <  2:1263470/54‑1 (MQ=255)                                                                                                    ttGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaagagaaga                                                                                                               >  1:1263470/1‑54 (MQ=255)                                                                                                     tGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaa                         <  2:358179/139‑1 (MQ=255)                                                                                                       cGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaa                                                                                                                      <  1:545359/44‑1 (MQ=255)                                                                                                       cGAGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCaa                                                                                                                      >  2:545359/1‑44 (MQ=255)                                                                                                         aGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGAt                                                                                      <  1:607843/74‑1 (MQ=255)                                                                                                         aGCAAAAAAGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGAt                                                                                      >  2:607843/1‑74 (MQ=255)                                                                                                            aaaaaaGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGa                  <  2:685254/139‑1 (MQ=255)                                                                                                              aaaaGCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAg                >  1:1020236/1‑139 (MQ=255)                                                                                                                  gCCGCTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCg            <  2:1170988/139‑1 (MQ=255)                                                                                                                      cTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCga                                                      <  1:761717/93‑1 (MQ=255)                                                                                                                      cTACGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCga                                                      >  2:761717/1‑93 (MQ=255)                                                                                                                        aCGCTTTAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGActccc      >  2:1416273/1‑139 (MQ=255)                                                                                                                            tttAGCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGTGCGGCAAAAAAATAGAAATAGATGAAGACCGActcccctcc  <  1:1565493/139‑1 (MQ=255)                                                                                                                                gCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                                       >  1:1481047/1‑82 (MQ=255)                                                                                                                                gCTATACGCGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                                       <  2:1481047/82‑1 (MQ=255)                                                                                                                                        |                                                                                                                               CATAGCCTGGTAATCGCCGCCCTGTAGGCTACTTGATTCTATAGAAACAGAAAAAGGCAAACGCAGCCTTTTCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACCGACTCCCCTCC  >  NC_000913/2465144‑2465405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑