| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,639,156 | T→C | N43S (AAC→AGC) | yfgM ← | ancillary SecYEG translocon subunit, putative anti‑RcsB factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,639,156 | 0 | T | C | 98.3% | 224.5 / NA | 60 | N43S (AAC→AGC) | yfgM | ancillary SecYEG translocon subunit, putative anti‑RcsB factor |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (31/28); minor base A (0/1); total (31/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTG > NC_000913/2639023‑2639287 | aCCATAAGTATTTTTATTTTCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAg < 1:42232/139‑1 (MQ=255) tAAGTATTTTTATTTTCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAgc < 2:702585/139‑1 (MQ=255) aGTATTTTTATTTTCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAgcgc < 1:1187101/139‑1 (MQ=255) tatttttattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCgcca < 2:34542/139‑1 (MQ=255) tttttattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCgccagc > 1:25799/1‑139 (MQ=255) tttattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCgccagcca > 2:1240631/1‑139 (MQ=255) tttattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCgccagcca > 2:691742/1‑139 (MQ=255) tttattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCgccagcca > 1:53892/1‑139 (MQ=255) tttattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCgccagcca > 2:52575/1‑139 (MQ=255) tattttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAAt < 1:955758/139‑1 (MQ=255) attttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATc > 1:136582/1‑139 (MQ=255) ttCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGt > 2:1738693/1‑139 (MQ=255) cgccgGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCAcc < 1:771313/139‑1 (MQ=255) ccgGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGT‑ACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCa < 1:900676/139‑1 (MQ=255) gggATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCaa < 1:1738693/139‑1 (MQ=255) cgttttGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGGAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGATCGAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAAGCACCCCAACAGCCAGTGCt < 1:1180882/136‑1 (MQ=255) tttGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTg > 2:664994/1‑139 (MQ=255) cGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCaaaaaa > 2:179357/1‑139 (MQ=255) gCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAAt < 2:1557155/71‑1 (MQ=255) gCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAAt > 1:1557155/1‑71 (MQ=255) gCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTaa > 2:1761520/1‑139 (MQ=255) gCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTaa < 2:136582/139‑1 (MQ=255) gCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTaa > 2:1676345/1‑139 (MQ=255) gCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTaa < 1:1761520/139‑1 (MQ=255) cATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAAc < 1:179357/139‑1 (MQ=255) aTTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCa > 2:1435906/1‑92 (MQ=255) aTTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCa < 1:1435906/92‑1 (MQ=255) aTTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAAcc > 2:168083/1‑139 (MQ=255) tttGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCa > 1:386965/1‑77 (MQ=255) tttGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCa < 2:386965/77‑1 (MQ=255) tGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCtt < 1:664994/139‑1 (MQ=255) tGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCtt < 1:1240631/139‑1 (MQ=255) gCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCt < 2:25799/139‑1 (MQ=255) gagaAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAAt < 1:1400819/55‑1 (MQ=255) gagaAGCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAAt > 2:1400819/1‑55 (MQ=255) gCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCt > 2:1200458/1‑90 (MQ=255) gCGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCt < 1:1200458/90‑1 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCa > 2:64747/1‑77 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCa < 1:64747/77‑1 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTg > 1:107602/1‑92 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTg < 2:107602/92‑1 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGttt < 2:247408/137‑1 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGttt < 2:811938/137‑1 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGttt > 1:811938/1‑137 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGttt > 1:247408/1‑137 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTc > 1:1626161/1‑139 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTc > 1:1561960/1‑139 (MQ=255) cGGAGCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTc > 1:1257716/1‑139 (MQ=255) gCGTGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTtc > 1:974037/1‑139 (MQ=255) tGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTAcc > 2:1416979/1‑121 (MQ=255) tGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTAcc < 1:1416979/121‑1 (MQ=255) tGCAGAATCAACCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCg > 1:1302132/1‑139 (MQ=255) aCCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAAttt > 2:1718633/1‑136 (MQ=255) aCCTGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAAttt < 1:1718633/136‑1 (MQ=255) tGATGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGc < 1:1676345/139‑1 (MQ=255) aTGGCTGCTCCAGTAGCGCCAGCCAATCAGTGTGCCAACGCCCAAAATCACCCCAACAGcc < 2:189959/61‑1 (MQ=255) aTGGCTGCTCCAGTAGCGCCAGCCAATCAGTGTGCCAACGCCCAAAATCACCCCAACAGcc > 1:189959/1‑61 (MQ=255) aTGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCACCGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCg > 1:1101259/1‑98 (MQ=255) aTGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAATCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTg > 1:1489655/1‑139 (MQ=255) aTGGCTGCTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCg < 2:1101259/98‑1 (MQ=255) | ACCATAAGTATTTTTATTTTCAGCAGCAAATTTTTCCGCCGCCGGGATGCTATCCGGTTTGCCTTCGCTCACTGCGGTAACCGCATTTTGATAGGCAAGAGAAGCGGAGCGTGCAGAATCAACCTGATGGCTGTTCCAGTAGCGCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTG > NC_000913/2639023‑2639287 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |