Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 2,767,500 | T→C | intergenic (+145/‑210) | rnlB → / → yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,767,500 | 0 | T | C | 96.7% | 211.6 / NA | 61 | intergenic (+145/‑210) | rnlB/yfjP | CP4‑57 prophage, uncharacterized protein/CP4‑57 prophage, 50S ribosome‑binding GTPase family protein |
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (23/36); minor base A (2/0); total (25/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.64e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
TTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCA > NC_000913/2767365‑2767634 | ttctAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑cccc < 2:567187/139‑1 (MQ=255) aaCCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTaa < 2:1670581/139‑1 (MQ=255) aaTTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCTCCCCCACAGCATTCTGCAAAATGAAAACAGTTACCCCAGTCTAACTACGATTGAGT‑T‑CACACACCTTTAAACATATCaata > 1:1224162/1‑139 (MQ=255) cGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACCACGCCCTGCATAAGGAACACGTTTTTCACTGTGCACCCACGACTAATT‑C‑TCCCCCCCTATAAACTTCTAAATAAGGt > 1:667613/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAACCCCCCCCACAAAACGTCCTGCATAGGGAACACGTTTTCCCCTTTGCACCCCCGACTAAAA‑T‑CCCCCCCCTCTAAAcacaccaacaattt > 1:1618308/1‑126 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATCAGGAACACGTTTGCACCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTc > 1:1693609/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTATTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTc > 1:939129/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACCTCCTGCATATGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCACCCCCTTTAAACTTCTCAATAATGTc > 1:977375/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCACACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACAAAAT‑T‑ACCCCCCCTTCAAACTTCTCAACAATATc > 2:1360917/1‑139 (MQ=255) gCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CACCCCCTCACAACGTCCTGCATTGGGGACACGTTTTCCCCCGTGCACCCACCACTAAATAA‑CCCCCCCCTTTTAACTTCCCAATCATGt > 1:37350/1‑139 (MQ=255) atatTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGa < 2:1224162/139‑1 (MQ=255) atTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGaaa < 2:335298/139‑1 (MQ=255) agagacaggTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGaaa < 2:1365020/131‑1 (MQ=255) tAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATc < 2:868449/139‑1 (MQ=255) gTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTgg < 2:506889/128‑1 (MQ=255) gTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAAGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCAACAATTTTCTgg > 1:506889/1‑128 (MQ=255) tCCAATATGATCAAATAGCATTAA‑CCCCCTCTGCCCACGTCCTGCATAGGGAACACGTTTTCACCTGTGCACCCACGACAAATT‑T‑ACCCCCCCTTTAATCTTCTCAAATATGTCACCAAGTGTCTGGAAATCGTatgat > 1:109079/1‑139 (MQ=255) tCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat < 2:805932/136‑1 (MQ=255) tCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTat > 1:805932/1‑136 (MQ=255) tGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 2:875106/139‑1 (MQ=255) tCAAATAGCATTAA‑CCCGCCCAAACAACGTCGTTCAGCAGGAACAGGTCTTCCTCTTAGAACCCTCTGATAAAT‑C‑CCCCGCCCTTAGATCTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTGTctct > 1:1365020/1‑136 (MQ=255) tCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACATCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCg > 2:699593/1‑139 (MQ=255) tAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGAGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTAGGATGCCTTTCCCGGTaaa < 1:992768/139‑1 (MQ=255) aGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAAt < 2:939129/139‑1 (MQ=255) cccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACGCCCGACTAAAT‑T‑CCCCCCCCCTTTAACGCTTCCATAATGTCACTAATTGTCTGGAAATCCTTTGATGCCTTTCACGATAAACGGTGCtgttcgt > 1:840380/1‑135 (MQ=255) ccTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGtt < 2:374972/139‑1 (MQ=255) tCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg < 1:1350530/135‑1 (MQ=255) tCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg > 2:1350530/1‑135 (MQ=255) caACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGaa > 1:1556902/1‑139 (MQ=255) aCGTCCTGCATTGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAg < 1:1360917/139‑1 (MQ=255) cGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa > 2:185650/1‑139 (MQ=255) cGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa < 2:1556902/139‑1 (MQ=255) cGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTGTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGa < 1:311101/139‑1 (MQ=255) tCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTa > 2:1441815/1‑96 (MQ=255) tCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTa < 1:1441815/96‑1 (MQ=255) tCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGAtt > 2:499023/1‑139 (MQ=255) cTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCt > 2:212804/1‑139 (MQ=255) tGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTg < 2:1618308/139‑1 (MQ=255) gCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGc < 1:212804/139‑1 (MQ=255) cATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc < 2:75199/102‑1 (MQ=255) cATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTc > 1:75199/1‑102 (MQ=255) cATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCa < 1:499023/139‑1 (MQ=255) aTAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCaa < 1:699593/139‑1 (MQ=255) gAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTAt < 2:840380/139‑1 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt > 1:1165611/1‑109 (MQ=255) acGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt < 2:1165611/109‑1 (MQ=255) tttCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAg > 2:75630/1‑117 (MQ=255) tttCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAg < 1:75630/117‑1 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt < 1:1310594/104‑1 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt > 2:1310594/1‑104 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt < 1:498674/104‑1 (MQ=255) ttCCCCTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCtgt > 2:498674/1‑104 (MQ=255) cccTGTGCACCCACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGaaaa < 2:977375/139‑1 (MQ=255) ccACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg > 2:1614157/1‑94 (MQ=255) ccACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAg < 1:1614157/94‑1 (MQ=255) ctcGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCTATAATGTTACCAATTTGCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 1:167028/137‑1 (MQ=255) cACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 2:1531856/139‑1 (MQ=255) cACGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagat < 2:1703801/139‑1 (MQ=255) aCGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAg > 2:396043/1‑97 (MQ=255) aCGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAg < 1:396043/97‑1 (MQ=255) aCGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagata > 2:1111809/1‑139 (MQ=255) cGACTAAAT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagatag > 2:1283514/1‑139 (MQ=255) aCTAAAT‑TCCCCCCCCCTTTTAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCagataga < 2:37350/139‑1 (MQ=255) aaT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCa < 2:109079/139‑1 (MQ=255) aaT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCa < 2:667613/139‑1 (MQ=255) aaT‑T‑CCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCa < 1:185650/139‑1 (MQ=255) | TTCTAAACGAACCAGTATAGAATTCGCCCTTTTGGCATGATATTAACTTGTCCAATATGATCAAATAGCATTAA‑CCCCCCCTCACAACGTCCTGCATAGGGAACACGTTTTCCCCTGTGCACCCACGACTAAAT‑T‑TCCCCCCCTTTAAACTTCTCAATAATGTCACCAATTTTCTGGAAATCCTATGATGCCTTTCCCGGTAAATGGTGCTGTGAGTTCAGAAAGATTCTGCAATCTATGTGCATTGAAAAAATCTTCAGATAGATATCA > NC_000913/2767365‑2767634 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |