Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,842,591 T→C L151L (TTA→TTG)  hycI ← protease involved in processing C‑terminal end of HycE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,842,5910TC100.0% 325.7 / NA 85L151L (TTA→TTG) hycIprotease involved in processing C‑terminal end of HycE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (45/40);  total (45/40)

CACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCAC  >  NC_000913/2842463‑2842722
                                                                                                                                |                                                                                                                                    
cACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGa                                                                                                                            >  1:1447422/1‑139 (MQ=255)
 aCGAACCATGAAGGCCCGATAAGCGCAGCCCATCGGGCAATTTAGGATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGaa                                                                                                                           >  1:1031226/1‑139 (MQ=255)
      ccATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgc                                                                                                                      <  2:971541/139‑1 (MQ=255)
       cATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTTATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgcc                                                                                                                     <  2:1749724/139‑1 (MQ=255)
       cATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgcc                                                                                                                     >  1:599788/1‑139 (MQ=255)
               cccGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATttcctt                                                                                                             >  1:375320/1‑139 (MQ=255)
                    tAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg                                                                                                        <  2:1473894/139‑1 (MQ=255)
                     aaGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGc                                                                                                       >  1:1382303/1‑139 (MQ=255)
                        cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCtt                                                                                                    >  1:1787767/1‑139 (MQ=255)
                         gcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   <  1:598907/139‑1 (MQ=255)
                          cagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTcc                                                                                                  >  2:1465090/1‑139 (MQ=255)
                             cgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt                                                                                               <  2:1382303/139‑1 (MQ=255)
                               caTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCAGCCCATCAAGAACATCCCTGTCCTGTTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCg                                                                                             <  1:873750/139‑1 (MQ=255)
                                 tCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGtt                                                                                           >  1:1533881/1‑139 (MQ=255)
                                      cAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      <  2:1014742/139‑1 (MQ=255)
                                      cAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      <  1:324945/139‑1 (MQ=255)
                                          ttAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      >  1:1166514/1‑135 (MQ=255)
                                          ttAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      <  2:1166514/135‑1 (MQ=255)
                                            aGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGtt                                                                               <  2:91798/139‑1 (MQ=255)
                                                   ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaaa                                                                                     <  1:520938/127‑1 (MQ=255)
                                                   ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaaa                                                                                     >  2:520938/1‑127 (MQ=255)
                                                   ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCg                                                                        <  2:1644402/139‑1 (MQ=255)
                                                        cAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTccc                                                                                                          >  1:1327070/1‑101 (MQ=255)
                                                        cAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTccc                                                                                                          <  2:1327070/101‑1 (MQ=255)
                                                        cAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      >  2:1573313/1‑121 (MQ=255)
                                                        cAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      <  1:1573313/121‑1 (MQ=255)
                                                                     ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTggg                                                      >  2:503543/1‑139 (MQ=255)
                                                                        cATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCa                                                   <  2:1533881/139‑1 (MQ=255)
                                                                          tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATc                                                 >  1:234902/1‑139 (MQ=255)
                                                                          tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCATTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATc                                                 >  1:1130081/1‑139 (MQ=255)
                                                                                    cccTGTCCTGATTCGTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCGAGCCTTCCAGTCGTTGATAAAC‑GGTTGCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTaaa                                       >  2:1083536/1‑139 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCAttt                                                                                                                 >  2:619253/1‑66 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCAttt                                                                                                                 <  1:619253/66‑1 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCAtt                                                                                                                  >  2:1650842/1‑65 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCAtt                                                                                                                  <  1:1650842/65‑1 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCat                                                                                                        <  2:1099769/75‑2 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGc                                                          >  2:1576655/1‑120 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGc                                                          <  1:1576655/120‑1 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTaaa                                       >  1:1663616/1‑139 (MQ=255)
                                                                                    cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCACGAAGCCGCCATTTCCTTCCCAg                                                                                                        >  1:1099769/1‑75 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg                                                                                                        <  2:335720/65‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg                                                                                                        >  1:335720/1‑65 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg                                                                                                        >  1:148255/1‑65 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAg                                                                                                        <  2:148255/65‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt                                                                                               >  2:153733/1‑74 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt                                                                                               <  1:153733/74‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCcgacga                              >  2:661739/1‑139 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTAcc                                                                         <  1:102462/95‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTAcc                                                                         >  2:102462/1‑95 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCa                                                   >  2:1320911/1‑117 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCa                                                   <  1:1320911/117‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCggg                                              >  2:760333/1‑122 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCggg                                              <  1:760333/122‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCgg                                               <  1:181338/121‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCgg                                               >  2:181338/1‑121 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagta                                         <  1:1713199/127‑1 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagta                                         >  2:1713199/1‑127 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat                             >  2:737349/1‑139 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat                             >  2:1477258/1‑139 (MQ=255)
                                                                                              aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGat                             >  1:1755972/1‑139 (MQ=255)
                                                                                                   ttAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgc                                                                                                                      >  2:711170/1‑46 (MQ=255)
                                                                                                   ttAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccgc                                                                                                                      <  1:711170/46‑1 (MQ=255)
                                                                                                   ttAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccg                                                                                                                       >  1:1744731/1‑45 (MQ=255)
                                                                                                   ttAATGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAgccg                                                                                                                       <  2:1744731/45‑1 (MQ=255)
                                                                                                     aaTGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt                                                                                               <  2:1202659/67‑1 (MQ=255)
                                                                                                     aaTGAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt                                                                                               >  1:1202659/1‑67 (MQ=255)
                                                                                                        gAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGttt                                                                              >  2:148099/1‑80 (MQ=255)
                                                                                                        gAAAAACTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGttt                                                                              <  1:148099/80‑1 (MQ=255)
                                                                                                            aaCTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCt                                                                            >  1:999294/1‑78 (MQ=255)
                                                                                                            aaCTACTCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCt                                                                            <  2:999294/78‑1 (MQ=255)
                                                                                                              ctactCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCg             <  2:857676/139‑1 (MQ=255)
                                                                                                               tactCTTCTTCCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGa            <  2:978011/139‑1 (MQ=255)
                                                                                                                        tcCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   >  1:255840/1‑44 (MQ=255)
                                                                                                                        tcCACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   <  2:255840/44‑1 (MQ=255)
                                                                                                                         ccACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      >  1:1206387/1‑56 (MQ=255)
                                                                                                                         ccACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                                      <  2:1206387/56‑1 (MQ=255)
                                                                                                                         ccACCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCAc  >  1:225205/1‑139 (MQ=255)
                                                                                                                           aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   >  2:568107/1‑41 (MQ=255)
                                                                                                                           aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   <  1:568107/41‑1 (MQ=255)
                                                                                                                           aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCAt                                                  >  2:66757/1‑89 (MQ=255)
                                                                                                                           aCCGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCAt                                                  <  1:66757/89‑1 (MQ=255)
                                                                                                                            ccGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   <  2:552225/40‑1 (MQ=255)
                                                                                                                            ccGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                                   >  1:552225/1‑40 (MQ=255)
                                                                                                                            ccGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAAt                                                              >  2:1180303/1‑76 (MQ=255)
                                                                                                                            ccGCCAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAAt                                                              <  1:1180303/76‑1 (MQ=255)
                                                                                                                                |                                                                                                                                    
CACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAC‑GGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGCCGAGGAAAATCAC  >  NC_000913/2842463‑2842722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑