Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,966,732 G→A A568V (GCA→GTA)  ptsP ← PEP‑protein phosphotransferase enzyme I, GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,966,7320GA100.0% 305.4 / NA 87A568V (GCA→GTA) ptsPPEP‑protein phosphotransferase enzyme I, GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/44);  total (43/44)

ACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCAC  >  NC_000913/2966600‑2966867
                                                                                                                                    |                                                                                                                                       
aCCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTtcat                                                                                                                                   >  2:669587/1‑139 (MQ=255)
 cccGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTtcatc                                                                                                                                  >  2:804745/1‑139 (MQ=255)
     cTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACt                                                                                                                              <  1:669587/139‑1 (MQ=255)
           cAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCg                                                                                                                        <  2:693991/139‑1 (MQ=255)
            aGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGTATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGa                                                                                                                       <  1:1280181/139‑1 (MQ=255)
             gATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAg                                                                                                                      <  1:441612/139‑1 (MQ=255)
               tGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCg                                                                                                                        >  2:173605/1‑135 (MQ=255)
               tGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCg                                                                                                                        <  1:173605/135‑1 (MQ=255)
                gCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCt                                                                                                                   >  1:1406011/1‑139 (MQ=255)
                    cagcaTAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTtgtg                                                                                                               >  1:559292/1‑139 (MQ=255)
                            aTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCgg                                                                                                       <  2:1749834/139‑1 (MQ=255)
                            aTACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCgg                                                                                                       <  1:1534927/139‑1 (MQ=255)
                               ccATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCaa                                                                                                    <  1:286018/139‑1 (MQ=255)
                                 aTTGACGGCACTTCCAGCATGATGCCGATTCGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGGCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  >  2:1193791/1‑139 (MQ=255)
                                 aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  >  2:1380658/1‑139 (MQ=255)
                                 aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  >  2:973775/1‑139 (MQ=255)
                                 aTTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  <  1:1355010/139‑1 (MQ=255)
                                  ttGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                                                                                 >  1:534496/1‑139 (MQ=255)
                                   tGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCg                                                                                                                        >  1:936913/1‑115 (MQ=255)
                                   tGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCg                                                                                                                        <  2:936913/115‑1 (MQ=255)
                                   tGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTtg                                                                                                                 <  1:1508544/122‑1 (MQ=255)
                                   tGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTtg                                                                                                                 >  2:1508544/1‑122 (MQ=255)
                                      cGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAata                                                                                             >  2:1425080/1‑139 (MQ=255)
                                       ggCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  >  1:166308/1‑133 (MQ=255)
                                       ggCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  <  2:166308/133‑1 (MQ=255)
                                          aCTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCa                                                                                         <  1:254508/139‑1 (MQ=255)
                                                      atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                                                                                     <  2:1242272/115‑1 (MQ=255)
                                                      atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                                                                                     <  1:103470/115‑1 (MQ=255)
                                                      atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                                                                                     >  2:103470/1‑115 (MQ=255)
                                                      atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCa                                                                                                     >  1:1242272/1‑115 (MQ=255)
                                                      atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTc                                                                                          <  1:1228254/126‑1 (MQ=255)
                                                      atgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTc                                                                                          >  2:1228254/1‑126 (MQ=255)
                                                       tgCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGc                                                                            <  2:1406011/139‑1 (MQ=255)
                                                        gCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCg                                                                           >  1:504654/1‑139 (MQ=255)
                                                         ccGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCgg                                                                          <  2:559292/139‑1 (MQ=255)
                                                            aTACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCAt                                                                       >  1:103578/1‑139 (MQ=255)
                                                            aTACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCAt                                                                       <  1:1380658/139‑1 (MQ=255)
                                                                gTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGc                                                                   >  1:560838/1‑139 (MQ=255)
                                                                 tGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCa                                                                  >  1:976983/1‑139 (MQ=255)
                                                                 tGGTTTGCGAATTTCGTAACCGATGATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCAGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCa                                                                  >  2:1092772/1‑139 (MQ=255)
                                                                   gTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTtcat                                                                                                                                   <  2:1675110/72‑1 (MQ=255)
                                                                   gTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTtcat                                                                                                                                   >  1:1675110/1‑72 (MQ=255)
                                                                   gTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAg                                                                                                                      <  1:1438247/85‑1 (MQ=255)
                                                                   gTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAg                                                                                                                      >  2:1438247/1‑85 (MQ=255)
                                                                      tGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcag                                                             <  1:1425080/139‑1 (MQ=255)
                                                                            tttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTa                                                                     <  1:1652/125‑1 (MQ=255)
                                                                            tttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTa                                                                     >  2:1652/1‑125 (MQ=255)
                                                                             ttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGa                                                                                                              >  2:566387/1‑83 (MQ=255)
                                                                             ttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGa                                                                                                              <  1:566387/83‑1 (MQ=255)
                                                                             ttCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc                                                      <  2:1590463/139‑1 (MQ=255)
                                                                             ttCGTAACCGATAATCTCCTCGACTTCACGTCCGGCACGTTCAAGCAGGCGGCGTACGTCATCAACTTCAACGAGGCTTGTGACCATCGGGAACAGAGTATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc                                                      <  1:1363857/139‑1 (MQ=255)
                                                                              tCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGCTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCCGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGccc                                                     >  1:658267/1‑139 (MQ=255)
                                                                                  aaCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCAc                                                 <  2:976983/139‑1 (MQ=255)
                                                                                  aaCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCAGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCAc                                                 <  1:1092772/139‑1 (MQ=255)
                                                                                   aCCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCAcc                                                <  1:973775/139‑1 (MQ=255)
                                                                                       atcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  <  2:1332942/85‑1 (MQ=255)
                                                                                       atcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACa                                                                                                  >  1:1332942/1‑85 (MQ=255)
                                                                                       atcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagc                                                            >  2:237520/1‑123 (MQ=255)
                                                                                       atcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACgcagc                                                            <  1:237520/123‑1 (MQ=255)
                                                                                       atcatcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGa                                            <  1:804745/139‑1 (MQ=255)
                                                                                         catcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCAt                                                                                                                          <  2:940628/59‑1 (MQ=255)
                                                                                         catcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCAt                                                                                                                          >  1:940628/1‑59 (MQ=255)
                                                                                         catcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATc                                          >  2:1423832/1‑139 (MQ=255)
                                                                                         catcTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATc                                          <  2:534496/139‑1 (MQ=255)
                                                                                                 cGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGAcc                                                                                                            >  1:173850/1‑65 (MQ=255)
                                                                                                 cGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGAcc                                                                                                            <  2:173850/65‑1 (MQ=255)
                                                                                                     ttCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTcc                              <  2:1698979/139‑1 (MQ=255)
                                                                                                      tCACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCg                             <  2:504654/139‑1 (MQ=255)
                                                                                                       caagTCCGGCATGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTAGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCgg                            >  1:1035470/4‑139 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                                                                                 >  2:1669610/1‑70 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                                                                                 <  1:1669610/70‑1 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc                                                      >  1:1359803/1‑113 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc                                                      <  2:1359803/113‑1 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGa                                            <  2:1480529/123‑1 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGa                                            >  1:1480529/1‑123 (MQ=255)
                                                                                                       cACGTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCgg                            >  1:955941/1‑139 (MQ=255)
                                                                                                          gTCCGGCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTg                         >  2:1653949/1‑139 (MQ=255)
                                                                                                              ggCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc                                                      <  2:225568/106‑1 (MQ=255)
                                                                                                              ggCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGcc                                                      >  1:225568/1‑106 (MQ=255)
                                                                                                               gCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                                                                                 >  2:256524/1‑62 (MQ=255)
                                                                                                               gCACGTTCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAg                                                                                                 <  1:256524/62‑1 (MQ=255)
                                                                                                                    ttCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGAt                                  <  1:752032/120‑1 (MQ=255)
                                                                                                                    ttCAATCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGAt                                  >  2:752032/1‑120 (MQ=255)
                                                                                                                        aTCAGGCGGCGTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGc           >  2:331807/1‑139 (MQ=255)
                                                                                                                               ggcgTACTTCATCAACTTCATCGAGGCTTGTGATCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCAc                                                                 <  1:1262639/78‑1 (MQ=255)
                                                                                                                               ggcgTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCAc                                                                 >  2:1262639/1‑78 (MQ=255)
                                                                                                                                 cgTACTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCAc  >  1:458370/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                       
ACCCGCTTTGCCAGATGCGGCAGCATAAATACCATTGACGGCACTTCCAGCATGATGCCGATACGTGGTTTGGGAATTTCGTAACCGATCATCTCCTCGACTTCACGTCCGGCACGTTCAATCAGGCGGCGTGCTTCATCAACTTCATCGAGGCTTGTGACCATCGGCAACAGAATATTCAGGTTGCCCGTAGCGGCATTAGCACGCAGCATCGCCCGCACCTGGATCAAGAAGATCTCCGGCTGATCGAGCGTAATGCGAATCCCAC  >  NC_000913/2966600‑2966867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: