Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,043,412 G→A A71V (GCA→GTA)  yqfB ← UPF0267 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,043,4120GA100.0% 244.1 / NA 70A71V (GCA→GTA) yqfBUPF0267 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/33);  total (37/33)

AATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACTCGTCG  >  NC_000913/3043284‑3043546
                                                                                                                                |                                                                                                                                      
aaTTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtc                                                                                                                              >  2:149751/1‑139 (MQ=255)
      aaCTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcagtcag                                                                                                                        <  2:14444/139‑1 (MQ=255)
       aCTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGc                                                                                                                       <  1:1454773/139‑1 (MQ=255)
       aCTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGc                                                                                                                       >  1:1663387/1‑139 (MQ=255)
         tAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGt                                                                                                                     <  2:1323680/139‑1 (MQ=255)
            cAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGttt                                                                                                                                 <  2:810875/124‑1 (MQ=255)
            cAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGttt                                                                                                                                 >  1:810875/1‑124 (MQ=255)
            cAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATc                                                                                                                  >  2:1673439/1‑139 (MQ=255)
            cAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATc                                                                                                                  >  1:156499/1‑139 (MQ=255)
             aGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATcc                                                                                                                 <  1:149751/139‑1 (MQ=255)
                cGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACAt                                                                                                                                     >  1:137428/1‑116 (MQ=255)
                cGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACAt                                                                                                                                     <  2:137428/116‑1 (MQ=255)
                 gTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCg                                                                                                             >  1:56513/1‑139 (MQ=255)
                  tGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGt                                                                                                            >  2:1025986/1‑139 (MQ=255)
                    aGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTa                                                                                                          >  1:1635740/1‑139 (MQ=255)
                    aGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTa                                                                                                          >  1:1506613/1‑139 (MQ=255)
                    aGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTa                                                                                                          >  2:656985/1‑139 (MQ=255)
                          aTTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTg                                                                                                    >  1:204774/1‑139 (MQ=255)
                                aGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTg                                                                                              >  2:1331232/1‑139 (MQ=255)
                                  aCATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  2:404353/139‑1 (MQ=255)
                                   cATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            <  2:1574071/106‑1 (MQ=255)
                                   cATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            >  1:1574071/1‑106 (MQ=255)
                                    aTTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGt                                                                                          <  2:794488/139‑1 (MQ=255)
                                    aTTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGt                                                                                          <  1:453928/139‑1 (MQ=255)
                                        aaaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACAt                                                                                                                                     <  1:603908/92‑1 (MQ=255)
                                        aaaTTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACAt                                                                                                                                     >  2:603908/1‑92 (MQ=255)
                                            tCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCaa                                                                                  <  2:1506613/139‑1 (MQ=255)
                                                              gtCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                >  2:1724560/1‑139 (MQ=255)
                                                              gtCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                <  2:355275/139‑1 (MQ=255)
                                                               tCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTc                                                               >  2:1606302/1‑139 (MQ=255)
                                                                cTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            <  1:783748/77‑1 (MQ=255)
                                                                cTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            >  2:783748/1‑77 (MQ=255)
                                                                  gACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGt                                                                                                      <  1:35819/97‑1 (MQ=255)
                                                                  gACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGt                                                                                                      >  2:35819/1‑97 (MQ=255)
                                                                             aTGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            <  2:626537/64‑1 (MQ=255)
                                                                             aTGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcag                                                                                                                            >  1:626537/1‑64 (MQ=255)
                                                                               gTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCg                                               >  1:1530405/1‑139 (MQ=255)
                                                                                 cAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGac                                             >  1:1563101/1‑139 (MQ=255)
                                                                                 cAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGac                                             <  1:619116/139‑1 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcagtcag                                                                                                                        >  2:445734/1‑63 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTtcagtcag                                                                                                                        <  1:445734/63‑1 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTa                                                                                                                    >  1:552427/1‑67 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTa                                                                                                                    <  2:552427/67‑1 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTa                                                                                                          >  2:76093/1‑77 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTa                                                                                                          <  1:76093/77‑1 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTc                                                 <  2:1761690/134‑1 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTc                                                 >  1:1761690/1‑134 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGaca                                            >  2:650024/1‑139 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGaca                                            <  2:1530129/139‑1 (MQ=255)
                                                                                  aGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGaca                                            >  1:1567745/1‑139 (MQ=255)
                                                                                       tGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                >  2:96685/1‑114 (MQ=255)
                                                                                       tGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGt                                                                <  1:96685/114‑1 (MQ=255)
                                                                                         aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACg                                                                                                        <  2:953152/72‑1 (MQ=255)
                                                                                         aCTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACg                                                                                                        >  1:953152/1‑72 (MQ=255)
                                                                                              tttCAGTTCAGTCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCa                                >  2:691384/1‑139 (MQ=255)
                                                                                               ttcagttcagTCATGGTCATATTTTCCTGTTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCAc                               <  1:458918/139‑1 (MQ=255)
                                                                                                   gttcagtCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            >  2:952666/1‑74 (MQ=255)
                                                                                                   gttcagtCAGGGTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  1:952666/74‑1 (MQ=255)
                                                                                                            gggTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTg                  <  2:204774/139‑1 (MQ=255)
                                                                                                            gggTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTg                  <  2:56513/139‑1 (MQ=255)
                                                                                                            gggTCATATTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTg                  <  2:156499/139‑1 (MQ=255)
                                                                                                                 atatTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGt                                                                                                            <  2:1696789/44‑1 (MQ=255)
                                                                                                                 atatTTTCCTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGt                                                                                                            >  1:1696789/1‑44 (MQ=255)
                                                                                                                         cTGCTCTACATGTTTTTCAGTCAGCGTATCCAGCGTTACGTTTGAGTTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACtcg     >  1:1714773/1‑139 (MQ=255)
                                                                                                                            ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            >  1:503019/1‑49 (MQ=255)
                                                                                                                            ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCg                                                                                            <  2:503019/49‑1 (MQ=255)
                                                                                                                            ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACtcgtcg  >  2:1240654/1‑139 (MQ=255)
                                                                                                                            ctctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACtcgtcg  <  1:1240654/139‑1 (MQ=255)
                                                                                                                             tctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTg                  >  1:225475/1‑122 (MQ=255)
                                                                                                                             tctACATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTg                  <  2:225475/122‑1 (MQ=255)
                                                                                                                                |                                                                                                                                      
AATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGTCAGGGTCATATTTTCCTGCTCTGCATGTTTTTCAGTCAGCGTATCCAGCGTTACGGTTGAGGTTGCGGTGACTTCAATCGTGCAAAAATAACCGTCATCTTCAAAACGTCCGACACGAAGCACATCACCCGTTTTGAAGTGCGATTCAGACTCGTCG  >  NC_000913/3043284‑3043546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: