Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,583	G→A	R286H (CGT→CAT)	yghU →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,583	0	G	A	100.0%	170.2 / NA	50	R286H (CGT→CAT)	yghU	putative S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/20);  total (30/20)

CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCA  >  NC_000913/3139447‑3139718                                                                                                                                         |                                                                                                                                        cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       <  2:81425/139‑1 (MQ=255) cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       <  2:1779884/139‑1 (MQ=255) cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       >  1:1710521/1‑139 (MQ=255)   cTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAg                                                                                                                                     >  2:891957/1‑139 (MQ=255)      ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAgggg                                                                                                                                  >  1:237995/1‑139 (MQ=255)      ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAgggg                                                                                                                                  >  1:1533109/1‑139 (MQ=255)                        aCGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtcgtc                                                                                                                >  2:1067776/1‑139 (MQ=255)                        aCGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtcgtc                                                                                                                >  1:1509254/1‑139 (MQ=255)                                     aaaCGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCTAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATc                                                                                                   <  1:1067776/139‑1 (MQ=255)                                         gTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAgg                                                                                               <  2:1177050/139‑1 (MQ=255)                                            ggCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       >  1:1410092/1‑96 (MQ=255)                                            ggCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       <  2:1410092/96‑1 (MQ=255)                                                          cGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       >  2:1073404/1‑82 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                                 <  1:1389593/86‑1 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                                 >  2:1389593/1‑86 (MQ=255)                                                            cACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgc                                                                            >  2:1710522/1‑139 (MQ=255)                                                             aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                       >  2:171073/1‑79 (MQ=255)                                                             aCCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAagcc                                                                           >  1:1774596/1‑139 (MQ=255)                                                                       cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtcgt                                                                                                                 >  1:1134086/1‑91 (MQ=255)                                                                       cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtcgt                                                                                                                 <  2:1134086/91‑1 (MQ=255)                                                                       cGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                 >  2:961035/1‑139 (MQ=255)                                                                         cTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGGAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt                                                               >  2:1437188/1‑139 (MQ=255)                                                                            aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccg                                                                          <  1:304785/125‑1 (MQ=255)                                                                            aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcgccg                                                                          >  2:304785/1‑125 (MQ=255)                                                                            aaTGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGt                                                            <  1:1343071/139‑1 (MQ=255)                                                                                 gCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca                                                       >  1:167242/1‑139 (MQ=255)                                                                                  cAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACaa                                                      >  1:1232057/1‑139 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGcc                                                  >  1:672906/1‑139 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGcc                                                  >  1:108386/1‑139 (MQ=255)                                                                                      tGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGcc                                                  >  1:1408442/1‑139 (MQ=255)                                                                                        cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTg                                                >  1:804048/1‑139 (MQ=255)                                                                                          tGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGAt                                              >  1:1269957/1‑139 (MQ=255)                                                                                               gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGc                                                                                              >  2:1530511/1‑86 (MQ=255)                                                                                               gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGc                                                                                              <  1:1530511/86‑1 (MQ=255)                                                                                               gcCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGAc                                         >  1:675414/1‑139 (MQ=255)                                                                                                ccATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACg                                        <  2:1533109/139‑1 (MQ=255)                                                                                                    gACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGc                                    <  2:1269957/139‑1 (MQ=255)                                                                                                       gCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCa                                                                                                                                      >  2:265082/1‑38 (MQ=255)                                                                                                       gCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCa                                                                                                                                      <  1:265082/38‑1 (MQ=38)                                                                                                              aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTAt                          <  2:675414/139‑1 (MQ=255)                                                                                                               tttCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATc                         <  2:237995/139‑1 (MQ=255)                                                                                                                  cGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAgg                      <  2:1232057/139‑1 (MQ=255)                                                                                                                        gAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACg                <  2:167242/139‑1 (MQ=255)                                                                                                                         aaTACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGa               <  2:1710521/139‑1 (MQ=255)                                                                                                                           tACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGaaa             >  1:492493/1‑139 (MQ=255)                                                                                                                                aaGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTaa        <  2:492493/139‑1 (MQ=255)                                                                                                                                  gATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACAAAAGCTAatc      >  2:404246/1‑139 (MQ=255)                                                                                                                                   aTAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatca     >  2:260181/1‑139 (MQ=255)                                                                                                                                     aaGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatc   >  2:271610/1‑139 (MQ=255)                                                                                                                                      aGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAAtcatca  <  2:1509254/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCAGTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCA  >  NC_000913/3139447‑3139718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑