Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,263,580	A→G	C27C (TGT→TGC)	tdcD ←	propionate kinase/acetate kinase C, anaerobic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,263,580	0	A	G	100.0%	307.6 / NA	80	C27C (TGT→TGC)	tdcD	propionate kinase/acetate kinase C, anaerobic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/41);  total (39/41)

TCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAG  >  NC_000913/3263446‑3263712                                                                                                                                       |                                                                                                                                     tCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGt                                                                                                                                  >  1:533332/1‑139 (MQ=255) tCCAGGTGAATTGGAGTTGGCTTCAGTGCACCTTCGTAGCTGTGGTGAGCCCGCGGCGACGGAGCTGCCGCCTTTACAGATGATGGTGCATTGTACCGGTTAATACCACCGGCAATAGCTCACATTCATACTGCGCAgc                                                                                                                                  <  1:1270731/139‑2 (MQ=255)           aTGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATc                                                                                                                        <  2:948803/139‑1 (MQ=255)                  tGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACg                                                                                                                 <  2:1382627/139‑1 (MQ=255)                      ttCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGaaa                                                                                                             >  1:936766/1‑139 (MQ=255)                                  tCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCg                                                                                                                                >  2:1760094/1‑108 (MQ=255)                                  tCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCg                                                                                                                                <  1:1760094/108‑1 (MQ=255)                                     tAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACg                                                                                              >  1:760551/1‑139 (MQ=255)                                        cTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                                                                                           <  2:448189/139‑1 (MQ=255)                                          gtggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGTCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATc                                                                                                     <  1:828290/127‑1 (MQ=255)                                          gtggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGTCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATc                                                                                                     >  2:828290/1‑127 (MQ=255)                                          gtggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca                                                                                         >  1:1429400/1‑139 (MQ=255)                                           tggtgAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac                                                                                        >  2:1666800/1‑139 (MQ=255)                                                aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca                                                                                         >  2:145843/1‑133 (MQ=255)                                                aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca                                                                                         >  1:400806/1‑133 (MQ=255)                                                aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca                                                                                         <  1:145843/133‑1 (MQ=255)                                                aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACca                                                                                         <  2:400806/133‑1 (MQ=255)                                                aGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTta                                                                                   >  1:1472948/1‑139 (MQ=255)                                                 gCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaa                                                                                  <  2:760551/139‑1 (MQ=255)                                                     gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                                                                                           >  2:892177/1‑126 (MQ=255)                                                     gCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                                                                                           <  1:892177/126‑1 (MQ=255)                                                         tGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaa                                                                          <  1:1344544/139‑1 (MQ=255)                                                          gCTGGCTCTCCCCCATTTACGGATAAGAATACATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCaaaa                                                                         >  1:735471/1‑139 (MQ=255)                                                           cTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAaac                                                                        >  2:1550255/1‑139 (MQ=255)                                                               ctctCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCg                                                                                                    <  2:818326/107‑1 (MQ=255)                                                               ctctCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCg                                                                                                    >  1:818326/1‑107 (MQ=255)                                                                tctcCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg                                                                   >  2:1239794/1‑139 (MQ=255)                                                                tctcCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCg                                                                   <  2:346644/139‑1 (MQ=255)                                                                   cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCg                                                                                                                                >  1:279570/1‑75 (MQ=255)                                                                   cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCg                                                                                                                                <  2:279570/75‑1 (MQ=255)                                                                   cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAg                                                                                                 >  1:799227/1‑106 (MQ=255)                                                                   cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAg                                                                                                 <  2:799227/106‑1 (MQ=255)                                                                   cccccATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGaa                                                                >  1:1409216/1‑139 (MQ=255)                                                                        aTTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattca                                                           <  1:210122/139‑1 (MQ=255)                                                                              ggATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtc                                                     >  2:1309091/1‑139 (MQ=255)                                                                                aTAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCAtctc                                                   >  1:698037/1‑139 (MQ=255)                                                                                       tGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTtctc                                            >  2:1409264/1‑139 (MQ=255)                                                                                        gCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCa                                           <  2:1499576/139‑1 (MQ=255)                                                                                          aTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                         >  1:884988/1‑139 (MQ=255)                                                                                          aTTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                         <  1:1239794/139‑1 (MQ=255)                                                                                            tttCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAG‑TAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTg                                      >  2:1653287/1‑139 (MQ=255)                                                                                              tCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattcatt                                                         <  1:1787015/119‑1 (MQ=255)                                                                                              tCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattcatt                                                         >  2:1787015/1‑119 (MQ=255)                                                                                               ccGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac                                                                                        <  2:1408535/87‑1 (MQ=255)                                                                                               ccGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACcac                                                                                        >  1:1408535/1‑87 (MQ=255)                                                                                                  aGTTAATACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTa                                 <  2:884988/139‑1 (MQ=255)                                                                                                      aaTACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCa                                                                                                                          <  2:1269377/46‑1 (MQ=255)                                                                                                      aaTACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCa                                                                                                                          >  1:1269377/1‑46 (MQ=255)                                                                                                      aaTACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGa                             >  1:150133/1‑139 (MQ=255)                                                                                                        tACCGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGAtt                           <  2:936766/139‑1 (MQ=255)                                                                                                           cGTCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTaaa                        <  2:533332/139‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                                                                                >  1:22857/1‑65 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                                                                                >  2:142340/1‑65 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                                                                                <  2:22857/65‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGa                                                                                                <  1:142340/65‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                                                                                           >  2:542978/1‑70 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAAc                                                                                           <  1:542978/70‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattcatt                                                         >  2:70778/1‑104 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattcatt                                                         <  1:70778/104‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                         <  2:674253/120‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATc                                         >  1:674253/1‑120 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc                      <  2:150133/139‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc                      <  2:698037/139‑1 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc                      >  1:1577144/1‑139 (MQ=255)                                                                                                             tCGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAAc                      >  1:836332/1‑139 (MQ=255)                                                                                                              cGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGa                                                                                                                      >  2:1208638/1‑42 (MQ=255)                                                                                                              cGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGa                                                                                                                      <  1:1208638/42‑1 (MQ=255)                                                                                                              cGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattca                                                           >  2:656565/1‑101 (MQ=255)                                                                                                              cGGCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAattca                                                           <  1:656565/101‑1 (MQ=255)                                                                                                               ggCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAG‑TAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGt                   <  1:1653287/139‑1 (MQ=255)                                                                                                                gCAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGt                   <  2:836332/139‑1 (MQ=255)                                                                                                                 cAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGTAAACTTAATCGAAGACGTACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGtt                  <  1:1147105/139‑1 (MQ=255)                                                                                                                 cAATACCTGACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGtt                  <  1:1666800/139‑1 (MQ=255)                                                                                                                         gACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaa                                                                                  <  2:1507804/67‑1 (MQ=255)                                                                                                                         gACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTtaa                                                                                  >  1:1507804/1‑67 (MQ=255)                                                                                                                         gACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACg          <  2:735471/139‑1 (MQ=255)                                                                                                                         gACATTAATACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACg          <  1:1550255/139‑1 (MQ=255)                                                                                                                                aTACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTa                                 <  2:2590/109‑1 (MQ=255)                                                                                                                                aTACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTa                                 >  1:2590/1‑109 (MQ=255)                                                                                                                                 tACTTCGCAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAg  >  2:383508/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                     TCCAGTTCAAATGCAATTGCCTTCAATGCACCTTCGTAGCTGTGGTGAGCCAGCGGTGCTGGCTCTCCCCCATTTACGGATAAGAATGCATTTTCCGAGTTAATACCGTCGGCAATACCTGACATTAATACTTCACAGTCGCTGGCATCGAGCACGGAAAACTTAATCGAAGACGAACCACAGTTAATAACCAAAACAACCGGAAATTCATTCATCTCTTTTCTCATCCTGAGTTACGGATTAAAACAGTTTGTATACGATGTTCAG  >  NC_000913/3263446‑3263712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑