Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,083	A→T	I74F (ATC→TTC)	acrE →	cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,083	0	A	T	100.0%	270.3 / NA	78	I74F (ATC→TTC)	acrE	cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (40/38);  total (40/38)

AGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGATCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCGAAAAGTGAAGCCGCCGC  >  NC_000913/3413947‑3414216                                                                                                                                         |                                                                                                                                      agaagagaaAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTc                                                                                                                                     >  1:1753603/1‑139 (MQ=255)   aagagaaAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGt                                                                                                                                   >  1:22548/1‑139 (MQ=255)      agaaAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACt                                                                                                                                <  1:63563/139‑1 (MQ=255)            cTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCg                                                                                                                          <  2:918054/139‑1 (MQ=255)             tCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGc                                                                                                                         >  1:844330/1‑139 (MQ=255)                cGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAAt                                                                                                                      >  2:959566/1‑139 (MQ=255)                 gTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCaa                                                                                                                       <  1:574912/137‑1 (MQ=255)                 gTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCaa                                                                                                                       >  2:574912/1‑137 (MQ=255)                             cAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGTATCGCAATTTCACTGAAGGCa                                                                                                         >  2:1050315/1‑139 (MQ=255)                             cAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAAt                                                                                                                            <  1:374917/120‑1 (MQ=255)                             cAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAAt                                                                                                                            >  2:374917/1‑120 (MQ=255)                                gTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCg                                                                                                      <  2:1753603/139‑1 (MQ=255)                                  tACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCTATTTCACTGAAGGCAGCGAt                                                                                                    <  1:1050315/139‑1 (MQ=255)                                  tACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCACAATTTc                                                                                                                   >  2:308938/1‑124 (MQ=255)                                  tACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCACAATTTc                                                                                                                   <  1:308938/124‑1 (MQ=255)                                   aCCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGAtg                                                                                                   >  1:850501/1‑139 (MQ=255)                                         cATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAg                                                                                             <  2:850501/139‑1 (MQ=255)                                          atatTGTAAAAACGGTCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAAt                                                                                                                            >  1:991222/1‑107 (MQ=255)                                          atatTGTAAAAACGGTCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAAt                                                                                                                            <  2:991222/107‑1 (MQ=255)                                               gTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCg                                                                                                                          <  2:1105213/104‑1 (MQ=255)                                               gTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCg                                                                                                                          >  1:1105213/1‑104 (MQ=255)                                                  aaaaCGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAAtt                                                                                                                     >  2:141723/1‑106 (MQ=255)                                                  aaaaCGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAAtt                                                                                                                     <  1:141723/106‑1 (MQ=255)                                                    aaCGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGCAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  1:1279310/139‑1 (MQ=255)                                                    aaCGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  2:22548/139‑1 (MQ=255)                                                    aaCGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  1:959566/139‑1 (MQ=255)                                                    aaCGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  2:584618/139‑1 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGa                                                                                                              >  2:1621095/1‑107 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGa                                                                                                              <  1:1621095/107‑1 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  >  2:453622/1‑135 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  2:454273/135‑1 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  >  1:454273/1‑135 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  1:453622/135‑1 (MQ=255)                                                        gCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGt                                                                              >  1:1526177/1‑139 (MQ=255)                                                          cccGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCCCAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCGGCGATGTGCAAGCAGGCCAGTCCCTGTAc                                                                            <  1:1211672/139‑1 (MQ=255)                                                          cccGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAg                                                                                             >  1:295518/1‑122 (MQ=255)                                                          cccGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAg                                                                                             <  2:295518/122‑1 (MQ=255)                                                          cccGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTAc                                                                            >  1:1505763/1‑139 (MQ=255)                                                                    gTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAgatcgatc                                                                  >  2:1247241/1‑139 (MQ=255)                                                                       aaGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCcg                                                               >  2:902079/1‑139 (MQ=255)                                                                       aaGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCcg                                                               >  1:556186/1‑139 (MQ=255)                                                                        aGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCcgc                                                              >  1:765469/1‑139 (MQ=255)                                                                         gACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCcgcg                                                             <  2:1284208/139‑1 (MQ=255)                                                                              aaTTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  >  1:97547/1‑113 (MQ=255)                                                                              aaTTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  2:97547/113‑1 (MQ=255)                                                                                   ccAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAgg                                                   <  2:765469/139‑1 (MQ=255)                                                                                   ccAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAgg                                                   >  1:1648257/1‑139 (MQ=255)                                                                                   ccAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAgg                                                   <  1:477506/139‑1 (MQ=255)                                                                                        ccGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTc                                                                                                                   <  1:91309/70‑1 (MQ=255)                                                                                        ccGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTc                                                                                                                   >  2:91309/1‑70 (MQ=255)                                                                                        ccGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAAt                                              >  1:204487/1‑139 (MQ=255)                                                                                        ccGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGAGACCTATCAGGCAAAt                                              >  1:690122/1‑139 (MQ=255)                                                                                              cAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGAc                                        <  1:1247241/139‑1 (MQ=255)                                                                                                   cTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAgcgc                                   <  2:1505763/139‑1 (MQ=255)                                                                                                      aTCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGcc                                                                                       >  2:642483/1‑84 (MQ=255)                                                                                                      aTCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGcc                                                                                       <  1:642483/84‑1 (MQ=255)                                                                                                      aTCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGaa                                >  1:776090/1‑139 (MQ=255)                                                                                                           ataGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCg                           >  2:1419229/1‑139 (MQ=255)                                                                                                            taGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGa                          >  2:1013454/1‑139 (MQ=255)                                                                                                             aGCCGAAGTTTGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAg                              >  2:381080/1‑134 (MQ=255)                                                                                                             aGCCGAAGTTTGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAg                              <  1:381080/134‑1 (MQ=255)                                                                                                             aGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTTAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATAACAGCGCGAAAGGCGaa                         <  2:690122/139‑1 (MQ=255)                                                                                                             aGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCa                                                                                      >  2:238040/1‑78 (MQ=255)                                                                                                             aGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCa                                                                                      <  1:238040/78‑1 (MQ=255)                                                                                                             aGCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGaa                         <  1:902079/139‑1 (MQ=255)                                                                                                              gCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTAc                                                                            >  1:637056/1‑87 (MQ=255)                                                                                                              gCCGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTAc                                                                            <  2:637056/87‑1 (MQ=255)                                                                                                               ccGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  >  2:422163/1‑80 (MQ=255)                                                                                                               ccGAAGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  1:422163/80‑1 (MQ=255)                                                                                                                  aaGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTAc                                                                            >  1:1026695/1‑83 (MQ=255)                                                                                                                  aaGTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTAc                                                                            <  2:1026695/83‑1 (MQ=255)                                                                                                                    gTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAg                                                                                             <  1:1000922/64‑1 (MQ=255)                                                                                                                    gTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAg                                                                                             >  2:1000922/1‑64 (MQ=255)                                                                                                                    gTTCGCCCACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCGa                  >  1:291048/1‑139 (MQ=255)                                                                                                                         cccACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  <  2:725446/70‑1 (MQ=255)                                                                                                                         cccACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTcc                                                                                  >  1:725446/1‑70 (MQ=255)                                                                                                                         cccACAGGTTAGCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGCCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCGAAAAGt             >  1:373847/1‑139 (MQ=255)                                                                                                                                    gCGGGTTCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCGAAAAGTGAAgccgccgc  <  2:1648257/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      AGAAGAGAAAGCTCACGTCGGTGAACCGCAGGTTACCGTTCATATTGTAAAAACGGCCCCGTTAGAAGTTAAGACTGAATTACCAGGCCGCACCAATGCTTATCGTATAGCCGAAGTTCGCCCACAGGTTAGCGGGATCGTACTGAATCGCAATTTCACTGAAGGCAGCGATGTGCAAGCAGGCCAGTCCCTGTACCAGATCGATCCCGCGACCTATCAGGCAAATTATGACAGCGCGAAAGGCGAACTGGCGAAAAGTGAAGCCGCCGC  >  NC_000913/3413947‑3414216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑