Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,113 A→G V306A (GTG→GCG)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,1130AG100.0% 277.9 / NA 74V306A (GTG→GCG) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/37);  total (37/37)

CTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCA  >  NC_000913/3439983‑3440245
                                                                                                                                  |                                                                                                                                    
ctctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTa                                                                                                                              >  1:1649093/1‑139 (MQ=255)
  ctTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAAt                                                                                                                            >  1:1007674/1‑139 (MQ=255)
   ttACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAAtc                                                                                                                           >  1:1005230/1‑139 (MQ=255)
           gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAag                                                                                                                   >  2:1628083/1‑139 (MQ=255)
           gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAag                                                                                                                   >  2:1567026/1‑139 (MQ=255)
           gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAag                                                                                                                   >  1:604246/1‑139 (MQ=255)
           gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAag                                                                                                                   >  1:358353/1‑139 (MQ=255)
           gcgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAag                                                                                                                   >  1:1544295/1‑139 (MQ=255)
            cgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAaga                                                                                                                  <  2:1007674/139‑1 (MQ=255)
            cgcATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAaga                                                                                                                  <  1:1628083/139‑1 (MQ=255)
                             ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGtt                                                                                                 >  2:1502342/1‑139 (MQ=255)
                             ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGtt                                                                                                 >  1:1468392/1‑139 (MQ=255)
                             ctcAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGtt                                                                                                 >  1:1346401/1‑139 (MQ=255)
                               cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                               <  1:1706370/139‑1 (MQ=255)
                               cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                               <  2:219573/139‑1 (MQ=255)
                               cAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCACATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAg                                                                                               <  2:1728009/139‑1 (MQ=255)
                                aGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAgg                                                                                              <  1:695959/139‑1 (MQ=255)
                                        cTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTaa                                                                                      <  2:1649093/139‑1 (MQ=255)
                                         ttAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAg                                                                                     <  2:1005230/139‑1 (MQ=255)
                                          tAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                                                    >  2:18052/1‑139 (MQ=255)
                                          tAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAgg                                                                                    >  2:886199/1‑139 (MQ=255)
                                                gtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAAt                                                                                                                            >  1:1465782/1‑93 (MQ=255)
                                                gtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAAt                                                                                                                            <  2:1465782/93‑1 (MQ=255)
                                                gtgATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCa                                                                              <  2:1346401/139‑1 (MQ=255)
                                                        gTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGt                                                                      >  1:422468/1‑139 (MQ=255)
                                                         ttACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTa                                                                     <  2:826199/139‑1 (MQ=255)
                                                          tACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTAc                                                                    <  2:1468392/139‑1 (MQ=255)
                                                           aCTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   <  2:1526677/139‑1 (MQ=255)
                                                           aCTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   >  2:1522619/1‑139 (MQ=255)
                                                           aCTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   <  2:358353/139‑1 (MQ=255)
                                                            cTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGc                                                                  <  1:1502342/139‑1 (MQ=255)
                                                                    cGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                                          <  2:604246/139‑1 (MQ=255)
                                                                      aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                        >  1:428565/1‑123 (MQ=255)
                                                                      aTGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                        <  2:428565/123‑1 (MQ=255)
                                                                       tGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   >  1:904257/1‑127 (MQ=255)
                                                                       tGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   <  2:904257/127‑1 (MQ=255)
                                                                         cTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCAc                                                     <  1:1567026/139‑1 (MQ=255)
                                                                              cGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                        <  1:666745/115‑1 (MQ=255)
                                                                              cGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCg                                                                        >  2:666745/1‑115 (MQ=255)
                                                                                gTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAaga                                                                                                                  <  1:1141902/71‑1 (MQ=255)
                                                                                gTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAaga                                                                                                                  >  2:1141902/1‑71 (MQ=255)
                                                                                 tGGCCAGTTTTCCAGGCGCATGCCCAGAAACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAg                                             >  1:1617513/1‑139 (MQ=255)
                                                                                            ccAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                                  <  2:169917/139‑1 (MQ=255)
                                                                                            ccAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTc                                  <  2:1544295/139‑1 (MQ=255)
                                                                                              aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                                          <  1:426902/113‑1 (MQ=255)
                                                                                              aGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                                          >  2:426902/1‑113 (MQ=255)
                                                                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                                       <  1:104413/96‑1 (MQ=255)
                                                                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                                       <  1:1498460/96‑1 (MQ=255)
                                                                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                                       >  2:1498460/1‑96 (MQ=255)
                                                                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCgg                                                                       >  2:104413/1‑96 (MQ=255)
                                                                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   >  2:468423/1‑100 (MQ=255)
                                                                                                  gcATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGAAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   <  1:468423/100‑1 (MQ=255)
                                                                                                   cATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   >  1:760200/1‑99 (MQ=255)
                                                                                                   cATGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   <  2:760200/99‑1 (MQ=255)
                                                                                                     tGCCCAGAGACAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAg                         >  1:1429721/1‑139 (MQ=255)
                                                                                                              aCAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGAtt                                                                                                              <  2:1489960/45‑1 (MQ=255)
                                                                                                              aCAGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGAtt                                                                                                              >  1:1489960/1‑45 (MQ=255)
                                                                                                                aGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                                <  1:925959/73‑1 (MQ=255)
                                                                                                                aGTCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCt                                                                                >  2:925959/1‑73 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTc                                                                               <  2:1435531/72‑1 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTc                                                                               >  1:1435531/1‑72 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACGTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTa                           <  2:1742715/124‑1 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   <  2:1412456/84‑1 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACg                                                                   >  1:1412456/1‑84 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                                          <  2:613922/93‑1 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAg                                                          >  1:613922/1‑93 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTa                           >  1:1742715/1‑124 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc                       <  2:517850/128‑1 (MQ=255)
                                                                                                                  tCCACGGGAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGc                       >  1:517850/1‑128 (MQ=255)
                                                                                                                       gggAAGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGAc       >  1:461288/1‑139 (MQ=255)
                                                                                                                          aaGCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGt    <  1:886199/139‑1 (MQ=255)
                                                                                                                            gCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                                        <  1:933910/85‑1 (MQ=255)
                                                                                                                            gCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGAt                                                        >  2:933910/1‑85 (MQ=255)
                                                                                                                            gCCAGCGCGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCa  >  2:535862/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                                    
CTCTTACGATGGCGCATGACCTTATCCTTCTCAGTAAAACCTTAACCTGTGATCCGGTTACTCGTCAGCGATGCTTGCCGGTGGCCAGTTTTCCAGGCGCATGCCCAGAGACAGTCCACGGGAAGCCAGCACGTCTTTAATCTCAGTAAGAGATTTTTTACCAAGGTTAGGCGTTTTAAGGAGCTCAACCTCGGTACGCTGTACCAGATCACCGATATAGTGGATAGCTTCTGCTTTAAGGCAGTTAGCAGAGCGGACAGTCA  >  NC_000913/3439983‑3440245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: