Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,457,094	A→G	I240V (ATC→GTC)	gspD →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,457,094	0	A	G	100.0%	333.1 / NA	88	I240V (ATC→GTC)	gspD	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/47);  total (41/47)

GGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGT  >  NC_000913/3456967‑3457227                                                                                                                                 |                                                                                                                                      ggAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGa                                                                                                                            <  2:218383/139‑1 (MQ=255)  gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGtcatca                                                                                                                                 >  1:220354/1‑133 (MQ=255)  gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGtcatca                                                                                                                                 <  2:220354/133‑1 (MQ=255)  gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGAc                                                                                                                           >  1:179172/1‑139 (MQ=255)       tGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGTCCGGaa                                                                                                                      >  2:557865/1‑139 (MQ=255)       tGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGaa                                                                                                                      >  1:727578/1‑139 (MQ=255)       tGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGaa                                                                                                                      >  1:942479/1‑139 (MQ=255)        gCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGaaa                                                                                                                     <  2:1004007/139‑1 (MQ=255)         cGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGaaaa                                                                                                                    >  1:1575713/1‑139 (MQ=255)             aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGa                                                                                                                            >  2:865451/1‑127 (MQ=255)             aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGa                                                                                                                            <  1:865451/127‑1 (MQ=255)             aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGaaa                                                                                                                     <  2:1459056/134‑1 (MQ=255)             aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGaaa                                                                                                                     >  1:1459056/1‑134 (MQ=255)             aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCa                                                                                                                >  2:839345/1‑139 (MQ=255)                ggAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGc                                                                                                             <  2:1616934/139‑1 (MQ=255)                     aTCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAgcg                                                                                                        <  2:179172/139‑1 (MQ=255)                        tcGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                                                                     <  2:1575713/139‑1 (MQ=255)                         cGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGa                                                                                                                       <  2:690231/120‑1 (MQ=255)                         cGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGa                                                                                                                       >  1:690231/1‑120 (MQ=255)                         cGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATc                                                                                                    >  2:994715/1‑139 (MQ=255)                          gCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCa                                                                                                   <  1:1474629/139‑1 (MQ=255)                                  tCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTa                                                                                           >  2:1567110/1‑139 (MQ=255)                                  tCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTa                                                                                           <  1:1488246/139‑1 (MQ=255)                                    ttAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACt                                                                                         >  2:1477490/1‑139 (MQ=255)                                          aaTTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                                                                     >  2:1479188/1‑121 (MQ=255)                                          aaTTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                                                                     <  1:1479188/121‑1 (MQ=255)                                          aaTTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAg                                                                                   >  2:1516681/1‑139 (MQ=255)                                            ttAATCAGCGAAAGTCACGGTAAAAGCCAGATGCCAGCCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGc                                                                                  >  1:1461211/1‑139 (MQ=255)                                               aTCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              <  2:351384/139‑1 (MQ=255)                                               aTCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              <  1:722609/139‑1 (MQ=255)                                               aTCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              <  1:722534/139‑1 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTgtcat                                                                                                                                   >  1:949409/1‑81 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTgtcat                                                                                                                                   <  2:949409/81‑1 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCt                                                                                <  1:604192/132‑1 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCt                                                                                >  2:1543624/1‑132 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCt                                                                                >  2:604192/1‑132 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCt                                                                                <  1:1543624/132‑1 (MQ=255)                                                    cGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTc                                                                         >  1:539165/1‑139 (MQ=255)                                                      aaaGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                                                                     >  1:214045/1‑109 (MQ=255)                                                      aaaGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                                                                     <  2:214045/109‑1 (MQ=255)                                                         gCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCt                                                                                >  2:400738/1‑127 (MQ=255)                                                         gCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCt                                                                                <  1:400738/127‑1 (MQ=255)                                                              ggTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCg                                                               >  2:1174986/1‑139 (MQ=255)                                                              ggTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCg                                                               >  1:1044840/1‑139 (MQ=255)                                                              ggTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCg                                                               >  1:941687/1‑139 (MQ=255)                                                                tAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGag                                                             <  2:539165/139‑1 (MQ=255)                                                                   aaGCCAGATGC‑‑G‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGaggaagg                                                        <  1:901059/139‑1 (MQ=255)                                                                        aGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              >  2:4835/1‑114 (MQ=255)                                                                        aGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              <  1:4835/114‑1 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGc                                                                                                             >  1:1159168/1‑81 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGc                                                                                                             <  2:1159168/81‑1 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCTAAGAGAGCGAGGAAGGAAATa                                                   >  2:1357448/1‑139 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAagagag                                                                 >  1:306222/1‑125 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAagagag                                                                 <  2:306222/125‑1 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGagg                                                            <  1:1575848/130‑1 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGagg                                                            >  2:1575848/1‑130 (MQ=255)                                                                          aTGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATa                                                   >  1:884264/1‑139 (MQ=255)                                                                            gCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATAcc                                                 <  2:1701707/139‑1 (MQ=255)                                                                               aG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCgg                                              >  1:83597/1‑139 (MQ=255)                                                                                  cccTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGt                                            <  2:727578/139‑1 (MQ=255)                                                                                  cccTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGt                                            <  1:671677/139‑1 (MQ=255)                                                                                     tcctcTCCGCGAAGATTGTGGCGGATAAGCGAACCGACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTtta                                         >  2:346927/1‑139 (MQ=255)                                                                                     tcctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGTCCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATATCCGGGTtta                                         <  1:557865/139‑1 (MQ=255)                                                                                     tcctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGa                                                                                                                       >  2:940730/1‑61 (MQ=255)                                                                                     tcctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGa                                                                                                                       <  1:940730/61‑1 (MQ=255)                                                                                      cctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTttat                                        <  2:83597/139‑1 (MQ=255)                                                                                          tcCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTAcc                                    <  2:1162809/139‑1 (MQ=255)                                                                                            cgcgAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTg                                  >  1:436817/1‑139 (MQ=255)                                                                                            cgcgAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTg                                  >  1:1732641/1‑139 (MQ=255)                                                                                                 aGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTa                                                                                           >  2:803828/1‑77 (MQ=255)                                                                                                 aGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTa                                                                                           <  1:803828/77‑1 (MQ=255)                                                                                                     tgtgGCGGATAAGCGAACCAACTCTCTTGTCATCAGAGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCt                         <  1:1174986/139‑1 (MQ=255)                                                                                                      gtgGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTa                        <  1:1658099/139‑1 (MQ=255)                                                                                                      gtgGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTGATTACCTGAAATATGCTa                        <  1:994715/139‑1 (MQ=255)                                                                                                       tgGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTaa                       <  2:941687/139‑1 (MQ=255)                                                                                                       tgGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTaa                       <  1:1477490/139‑1 (MQ=255)                                                                                                       tgGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTaa                       <  2:1461211/139‑1 (MQ=255)                                                                                                        ggCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAg                                                                                                          >  2:160344/1‑55 (MQ=255)                                                                                                        ggCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAg                                                                                                          <  1:160344/55‑1 (MQ=255)                                                                                                            gATAAGCGAACCGACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAgtca                  <  1:346927/139‑4 (MQ=255)                                                                                                             aTAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACt                                                                                         >  2:840488/1‑67 (MQ=255)                                                                                                             aTAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACt                                                                                         <  1:840488/67‑1 (MQ=255)                                                                                                                      ccAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              >  2:1078202/1‑69 (MQ=255)                                                                                                                      ccAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg                                                                              <  1:1078202/69‑1 (MQ=255)                                                                                                                       cAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCtggtg       <  2:942479/139‑1 (MQ=255)                                                                                                                         aCTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACg                <  1:1235407/128‑1 (MQ=255)                                                                                                                         aCTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACg                >  2:1235407/1‑128 (MQ=255)                                                                                                                          ctctctTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGagg                                                            <  2:309798/83‑1 (MQ=255)                                                                                                                          ctctctTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGagg                                                            >  1:309798/1‑83 (MQ=255)                                                                                                                            ctctTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGt  <  2:1732641/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                      GGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAG‑CCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGT  >  NC_000913/3456967‑3457227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑