Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 852,261 A→G Y113H (TAC→CAC)  opgE ← OPG biosynthetic transmembrane phosphoethanolamine transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913852,2610AG100.0% 277.0 / NA 72Y113H (TAC→CAC) opgEOPG biosynthetic transmembrane phosphoethanolamine transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/39);  total (33/39)

AGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAAATAACAAGCCGC  >  NC_000913/852130‑852384
                                                                                                                                   |                                                                                                                           
aGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTAc                                                                                                                      <  2:557550/139‑1 (MQ=255)
         aaaTGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGaaaa                                                                                                             <  2:992205/139‑1 (MQ=255)
                gCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCa                                                                                                      >  2:1467875/1‑139 (MQ=255)
                          aTACCAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGt                                                                                            <  2:1346277/139‑1 (MQ=255)
                             ccAGACTCAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  1:271133/139‑1 (MQ=255)
                             ccAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  1:498000/139‑1 (MQ=255)
                             ccAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  1:414845/139‑1 (MQ=255)
                             ccAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  1:1488385/139‑1 (MQ=255)
                             ccAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  2:1784944/139‑1 (MQ=255)
                             ccAGACACAGGCTGTCGAAGCTATTCCAGCGCGCTTTTTCATGCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAACCGTCAGGATTTCGGTCGa                                                                                         <  2:1321901/139‑1 (MQ=255)
                                gACACAGGCTGTCGAAGGTATTCCGGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGActc                                                                                      >  2:1092574/1‑139 (MQ=255)
                                gACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGActc                                                                                      >  1:1773607/1‑139 (MQ=255)
                                 acacAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGa                                                                                                   <  1:382835/125‑1 (MQ=255)
                                 acacAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGa                                                                                                   >  2:382835/1‑125 (MQ=255)
                                 acacAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGActct                                                                                     >  1:1753972/1‑139 (MQ=255)
                                   acaGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   >  1:1363678/1‑139 (MQ=255)
                                   acaGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   <  2:1753972/139‑1 (MQ=255)
                                       gCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCata                                                                               >  2:711258/1‑139 (MQ=255)
                                       gCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCa                                                                                 <  2:800734/137‑1 (MQ=255)
                                       gCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCa                                                                                 >  1:800734/1‑137 (MQ=255)
                                        cTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCatat                                                                              >  1:806176/1‑139 (MQ=255)
                                          gTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCACCTGATTGCGATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATtg                                                                            >  1:1102068/1‑139 (MQ=255)
                                           tCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATtgg                                                                           >  2:1220689/1‑139 (MQ=255)
                                            cGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   <  2:601068/130‑1 (MQ=255)
                                            cGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   >  1:601068/1‑130 (MQ=255)
                                                     tCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCaa                                                                 >  2:494620/1‑139 (MQ=255)
                                                      ccAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATGAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAAt                                                                <  1:1092574/139‑1 (MQ=255)
                                                          cgcgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         >  1:1020174/1‑110 (MQ=255)
                                                          cgcgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  2:1020174/110‑1 (MQ=255)
                                                           gcgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   >  2:699592/1‑115 (MQ=255)
                                                           gcgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   <  1:699592/115‑1 (MQ=255)
                                                            cgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGaaaa                                                                                                             <  2:1046193/88‑1 (MQ=255)
                                                            cgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGaaaa                                                                                                             >  1:1046193/1‑88 (MQ=255)
                                                            cgcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGc                                                          >  2:303410/1‑139 (MQ=255)
                                                             gcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  2:806176/139‑1 (MQ=255)
                                                             gcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  1:1691024/139‑1 (MQ=255)
                                                             gcTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  2:1363678/139‑1 (MQ=255)
                                                               tttttCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAAt                                                       >  1:73373/1‑139 (MQ=255)
                                                                               tttCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGgcg                                       <  2:893742/139‑1 (MQ=255)
                                                                               tttCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGgcg                                       <  2:73373/139‑1 (MQ=255)
                                                                               tttCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGgcg                                       <  2:1102068/139‑1 (MQ=255)
                                                                                     cTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGActct                                                                                     >  1:1558928/1‑87 (MQ=255)
                                                                                     cTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGActct                                                                                     <  2:1558928/87‑1 (MQ=255)
                                                                                                  cGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  2:1511179/70‑1 (MQ=255)
                                                                                                  cGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         >  1:1511179/1‑70 (MQ=255)
                                                                                                  cGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAAt                                                                <  2:1532204/95‑1 (MQ=255)
                                                                                                  cGCCGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAAt                                                                >  1:1532204/1‑95 (MQ=255)
                                                                                                    ccGAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAg                  >  1:121825/1‑139 (MQ=255)
                                                                                                      gAGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAggg                <  1:494620/139‑1 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTc                                                                                                       <  2:902861/51‑1 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTc                                                                                                       >  1:902861/1‑51 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         >  2:1344901/1‑65 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  1:1344901/65‑1 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  1:888274/97‑1 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         >  2:888274/1‑97 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  1:1346411/97‑1 (MQ=255)
                                                                                                       aGCGCAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         >  2:1346411/1‑97 (MQ=255)
                                                                                                         cgcAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   <  1:848987/69‑1 (MQ=255)
                                                                                                         cgcAAAAATAAATAAGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   >  2:848987/1‑69 (MQ=255)
                                                                                                                    ataaGCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAAATAACAAgccgc  <  1:1220689/139‑1 (MQ=255)
                                                                                                                        gCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         >  1:1386090/1‑48 (MQ=255)
                                                                                                                        gCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGa                                                                                         <  2:1386090/48‑1 (MQ=255)
                                                                                                                        gCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   <  2:1061486/54‑1 (MQ=255)
                                                                                                                        gCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTc                                                                                   >  1:1061486/1‑54 (MQ=255)
                                                                                                                        gCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGgcg                                       >  2:1723077/1‑98 (MQ=255)
                                                                                                                        gCCGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGgcg                                       <  1:1723077/98‑1 (MQ=255)
                                                                                                                          cGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         >  2:292444/1‑78 (MQ=255)
                                                                                                                          cGACCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  1:292444/78‑1 (MQ=255)
                                                                                                                            aCCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  1:493631/76‑1 (MQ=255)
                                                                                                                            aCCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         >  2:493631/1‑76 (MQ=255)
                                                                                                                            aCCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         <  2:1772360/76‑1 (MQ=255)
                                                                                                                            aCCAGGTGGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCa                                                         >  1:1772360/1‑76 (MQ=255)
                                                                                                                                   |                                                                                                                           
AGCAACAAAAAATGTCGCCACACTGAATACCAGACACAGGCTGTCGAAGGTATTCCAGCGCGCTTTTTCATTCTCTTTTTTTCGCCTGATTGCTATTACGCCGAGCGCAAAAATAAATAAGCCGACCAGGTAGCTGTACCACGGAAAAATCGTCAGGATTTCGGTCGACTCTTCCATATTGGTGGAGTGCAATGCCAGCAATGTATTGAAATTAGGCGCGCCGTAGGCCTGAGCAAAAGGGAAATAACAAGCCGC  >  NC_000913/852130‑852384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑