Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,748,385	G→A	A434A (GCG→GCA)	lyxK →	L‑xylulose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,385	0	G	A	100.0%	172.4 / NA	50	A434A (GCG→GCA)	lyxK	L‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

TTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTT  >  NC_000913/3748264‑3748518                                                                                                                          |                                                                                                                                      ttCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGt                                                                                                                      <  2:503701/139‑1 (MQ=255) ttCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGt                                                                                                                      <  1:60730/139‑1 (MQ=255)       cccTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCAc                                                                                                                <  1:310639/139‑1 (MQ=255)        ccTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGt                                                                                                                      <  2:1241275/132‑1 (MQ=255)        ccTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGt                                                                                                                      >  1:1241275/1‑132 (MQ=255)        ccTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCAcc                                                                                                               >  1:260394/1‑139 (MQ=255)          tACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGATGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCgg                                                                                                             >  2:1101443/1‑139 (MQ=255)           aCGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCggg                                                                                                            >  1:1512779/1‑139 (MQ=255)           aCGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCggg                                                                                                            >  2:358992/1‑139 (MQ=255)           aCGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCggg                                                                                                            >  1:507716/1‑139 (MQ=255)             gcgTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGt                                                                                                          <  1:965998/139‑1 (MQ=255)             gcgTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGt                                                                                                          <  1:247597/139‑1 (MQ=255)                 cACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTAt                                                                                                      <  1:1058514/139‑1 (MQ=255)                  aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGt                                                                                                          >  2:1742577/1‑134 (MQ=255)                  aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGt                                                                                                          <  1:1742577/134‑1 (MQ=255)                  aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATc                                                                                                     >  2:1431108/1‑139 (MQ=255)                  aCTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATc                                                                                                     >  2:1424165/1‑139 (MQ=255)                    tGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATcac                                                                                                   <  2:875420/139‑1 (MQ=255)                       cggcCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAAc                                                                                                >  1:199854/1‑139 (MQ=255)                               cgcACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGa                                                                                        <  1:1431108/139‑1 (MQ=255)                                    tCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGccc                                                                                   <  2:507716/139‑1 (MQ=255)                                    tCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGccc                                                                                   >  1:1567634/1‑139 (MQ=255)                                        aTGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACg                                                                               <  2:1512779/139‑1 (MQ=255)                                            cTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGAc                                                                           <  1:358992/139‑1 (MQ=255)                                              ggATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACtt                                                                         >  2:1337615/1‑139 (MQ=255)                                                      aTGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACAcc                                                                 <  2:260394/139‑1 (MQ=255)                                                         cTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTgccg                                                                                                                             <  1:353698/76‑1 (MQ=255)                                                         cTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTgccg                                                                                                                             >  2:353698/1‑76 (MQ=255)                                                               gACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCa                                                        <  1:1337615/139‑1 (MQ=255)                                                                aCGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAg                                                                                      >  1:1037670/1‑108 (MQ=255)                                                                aCGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAg                                                                                      <  2:1037670/108‑1 (MQ=255)                                                                       cGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCATAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctgctg                                                <  1:1101443/139‑1 (MQ=255)                                                                           cTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAg                                            >  2:1147095/1‑139 (MQ=255)                                                                              cGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctgctg                                                >  2:1165143/1‑132 (MQ=255)                                                                              cGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCctgctg                                                <  1:1165143/132‑1 (MQ=255)                                                                                  tCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGAc                                     <  1:789891/139‑1 (MQ=255)                                                                                    gAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGt                                                                                                                      <  1:61059/56‑1 (MQ=255)                                                                                    gAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGt                                                                                                                      >  2:61059/1‑56 (MQ=255)                                                                                    gAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCg                                   >  2:295480/1‑139 (MQ=255)                                                                                        tGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTAt                                                                                                      <  2:1543403/68‑1 (MQ=255)                                                                                        tGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTAt                                                                                                      >  1:1543403/1‑68 (MQ=255)                                                                                        tGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCa                               <  2:1452461/139‑1 (MQ=255)                                                                                        tGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCa                               <  2:1723381/139‑1 (MQ=255)                                                                                        tGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCa                               >  1:1527127/1‑139 (MQ=255)                                                                                                        aaCCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCaaaaa               <  2:1467505/139‑1 (MQ=255)                                                                                                         aCCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGacac                                                                  >  1:302505/1‑87 (MQ=255)                                                                                                         aCCGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGacac                                                                  <  2:302505/87‑1 (MQ=255)                                                                                                          ccGGCTGCTTTGGTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCaaaaaaa             >  1:544862/1‑139 (MQ=255)                                                                                                             gctgctTTGGGGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGGCCGCCCATCAGCTTTACCAAAAAAAata          <  1:1147095/139‑1 (MQ=255)                                                                                                                     ggTGCAGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGtt  <  1:17012/139‑1 (MQ=255)                                                                                                                          |                                                                                                                                      TTCACACCCTACGCGTCACTGGCGGCCCGGCGCACTCCGATGTCTGGATGCAAATGCTGGCGGACGTCAGCGGTCTGCGTATCGAGCTGCCGCAGGTGGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTT  >  NC_000913/3748264‑3748518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑