| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,207,900 | C→T | intergenic (‑368/‑247) | purH ← / → rrsE | IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase/16S ribosomal RNA of rrnE operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,207,900 | 0 | C | T | 98.1% | 166.9 / ‑6.6 | 53 | intergenic (‑368/‑247) | purH/rrsE | IMP cyclohydrolase and phosphoribosylaminoimidazolecarboxamide formyltransferase/16S ribosomal RNA of rrnE operon |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (28/24); minor base A (0/1); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGTATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGT > NC_000913/4207765‑4208034 | aGGTATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTcgg < 1:942162/139‑1 (MQ=255) gTATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTcggtt < 2:1756981/139‑1 (MQ=255) ggCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAaga > 1:1368257/1‑139 (MQ=255) tGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGaaaaa < 1:115634/139‑1 (MQ=255) tGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGaa < 1:1250441/121‑1 (MQ=255) tGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGaa > 2:1250441/1‑121 (MQ=255) gtcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTcggtt > 1:1247915/1‑106 (MQ=255) gtcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTcggtt < 2:1247915/106‑1 (MQ=255) tcagtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTg > 2:1382745/1‑139 (MQ=255) agtcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGAc < 1:335239/139‑1 (MQ=255) tcaTTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGActc > 1:153751/1‑139 (MQ=255) gCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGa < 1:1486259/139‑1 (MQ=255) gCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGa > 2:801467/1‑139 (MQ=255) ttttCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCg < 2:670614/139‑1 (MQ=255) cGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGc < 2:400077/139‑1 (MQ=255) cGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACATCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGc < 1:1736114/139‑1 (MQ=255) agaACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGa > 2:30091/1‑114 (MQ=17) agaACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGa < 1:30091/114‑1 (MQ=17) agaACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGa < 2:153751/139‑1 (MQ=17) agaACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGa < 1:730520/139‑1 (MQ=17) aaCTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACa < 1:1382745/139‑1 (MQ=11) aaCTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACGTCACACAAACAGACGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGTGAAGCGTAATATACGCCACCTCGCGACa < 1:783568/139‑1 (MQ=2) ccTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTgcg > 2:973109/1‑139 (MQ=14) ccTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTgcg > 2:321207/1‑139 (MQ=14) ccTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTgcg > 1:674585/1‑139 (MQ=14) ccTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTgcg > 1:1529653/1‑139 (MQ=14) tGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAgc > 1:655383/1‑139 (MQ=37) tGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAg > 2:448085/1‑138 (MQ=25) tGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAg < 1:448085/138‑1 (MQ=37) gcgcCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAgcg > 1:1350729/1‑139 (MQ=37) gcgcCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAgcg < 2:1368257/139‑1 (MQ=38) gcCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGcgcg > 1:480804/1‑139 (MQ=255) gcCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTAGCGACAGTGCGCTAAAGcgcg > 2:1050093/1‑139 (MQ=255) ccTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGt > 1:1710091/1‑139 (MQ=255) cTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTc > 1:111362/1‑139 (MQ=255) gcggATGTGAATCACTTCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCttt < 2:1529653/139‑1 (MQ=255) ttCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAg > 2:80200/1‑105 (MQ=255) ttCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAg < 1:80200/105‑1 (MQ=255) ttCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACa > 1:200268/1‑128 (MQ=255) ttCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACa < 2:200268/128‑1 (MQ=255) ttCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGAc > 2:409089/1‑139 (MQ=255) ttCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGAc > 1:926002/1‑139 (MQ=255) tCACACAAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACa < 2:111362/139‑1 (MQ=255) cacacaAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACa > 2:283596/1‑138 (MQ=255) cacacaAACAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCGTTAACAATTTATCAGACa < 1:283596/138‑1 (MQ=255) aaaCAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGct > 2:1638528/1‑113 (MQ=255) aaaCAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGct < 1:1638528/113‑1 (MQ=255) aaaCAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCtgt > 2:555968/1‑139 (MQ=255) aaCAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACa < 2:1275037/132‑1 (MQ=255) aaCAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACa > 1:1275037/1‑132 (MQ=255) aCAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCtgtgt > 2:1721664/1‑139 (MQ=255) cAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACa > 2:828144/1‑118 (MQ=255) cAGTCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACa < 1:828144/118‑1 (MQ=255) | AGGTATAAAGCGGGCTTTTGCTGGGGAATGCAGCAGTCAGTCATTTTTCTGCAATTTTTCTATTGCGGCCTGCGGAGAACTCCCTATAATGCGCCTCCATCGACACGGCGGATGTGAATCACTTCACACAAACAGCCGGTTCGGTTGAAGAGAAAAATCCTGAAATTCAGGGTTGACTCTGAAAGAGGAAAGCGTAATATACGCCACCTCGCGACAGTGCGCTAAAGCGCGTCGCAACTGCTCTTTAACAATTTATCAGACAATCTGTGT > NC_000913/4207765‑4208034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |