| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,229,765 | A→G | A126A (GCT→GCC) | pepE ← | peptidase E, alpha‑aspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,229,765 | 0 | A | G | 98.2% | 207.5 / NA | 57 | A126A (GCT→GCC) | pepE | peptidase E, alpha‑aspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (33/23); minor base T (0/1); total (33/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
ACGGGTTTCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGA > NC_000913/4229630‑4229898 | aCGGGTTTCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGggcaggca < 2:1035855/139‑1 (MQ=255) tCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAg > 2:372456/1‑139 (MQ=255) tCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAg > 2:420016/1‑139 (MQ=255) ccTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 2:1468251/139‑1 (MQ=255) ccTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 1:818847/139‑1 (MQ=255) ccTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 1:353956/139‑1 (MQ=255) ttCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 2:732305/139‑1 (MQ=255) gcgcGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAAtata > 1:1669672/1‑139 (MQ=255) gcgTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCAACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACa < 2:1199530/139‑1 (MQ=255) gTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACaga > 1:322544/1‑139 (MQ=255) ggTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc < 1:491332/122‑1 (MQ=255) ggTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTcc > 2:491332/1‑122 (MQ=255) gTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAgcgc < 2:322544/139‑1 (MQ=255) gTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAgcgc < 2:1337127/139‑1 (MQ=255) tGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAAc < 2:1278640/131‑1 (MQ=255) tGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGAGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAAc > 1:1278640/1‑131 (MQ=255) aGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTaa < 2:1256552/139‑1 (MQ=255) gCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAAt > 1:356111/1‑139 (MQ=255) ggAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAgc < 2:714472/85‑1 (MQ=255) ggAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAgc > 1:714472/1‑85 (MQ=255) gcATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGCACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCcgc > 2:559638/1‑139 (MQ=255) aTCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCgcgc < 1:420016/139‑1 (MQ=255) tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGTCAGGCAAGGTTAGCGCCTGCGCTCCAg > 2:419592/1‑81 (MQ=255) tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 1:419592/81‑1 (MQ=255) tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCt > 2:365188/1‑139 (MQ=255) tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCt > 1:563534/1‑139 (MQ=255) tCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCt > 1:378320/1‑139 (MQ=255) ccTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGc < 2:378320/139‑1 (MQ=255) cTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCa < 1:372456/139‑1 (MQ=255) cGGATCGACAATCGGCTTATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTaa < 1:830262/107‑1 (MQ=255) cGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTaa > 2:830262/1‑107 (MQ=255) cGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAg > 1:691200/1‑139 (MQ=255) gACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGc > 2:978404/1‑139 (MQ=255) gACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGc > 2:888896/1‑139 (MQ=255) cGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg > 1:1582917/1‑50 (MQ=255) cGTTGGTGGTACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 2:1582917/50‑1 (MQ=255) ggtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGttt > 1:1750751/1‑69 (MQ=255) ggtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGttt < 2:1750751/69‑1 (MQ=255) gtgttACGAATAGTTGGGCATGCAAGGTTAGCGCCTGCGCTCCAGCCa < 1:1743177/48‑1 (MQ=255) gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg < 2:948131/45‑1 (MQ=255) gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAg > 1:948131/1‑45 (MQ=255) gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCa > 2:1743177/1‑48 (MQ=255) gtggtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgc > 1:199970/1‑139 (MQ=255) gtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgc > 1:55109/1‑139 (MQ=255) gtACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgc > 2:732123/1‑139 (MQ=255) tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCaa < 2:1347793/118‑1 (MQ=255) tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCaa > 1:1347793/1‑118 (MQ=255) tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc > 1:905627/1‑139 (MQ=255) tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc > 1:728208/1‑139 (MQ=255) tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCACTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc > 2:1206688/1‑139 (MQ=255) tACGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCACTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgcc < 1:1206688/139‑1 (MQ=255) aCGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgccg > 1:1340037/1‑139 (MQ=255) aCGAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGAGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCccgccgccg > 1:1053774/1‑139 (MQ=255) gAATAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGAc > 2:1081296/1‑139 (MQ=255) tAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAAt < 2:784973/139‑1 (MQ=255) tAGTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAAt > 1:1621752/1‑139 (MQ=255) gTTGGGCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCa < 1:365188/139‑1 (MQ=255) gggCAGGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTAAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACACTCACGa > 1:1033555/1‑139 (MQ=255) | ACGGGTTTCACCTTTATGGCCTTCCGGCAGCGCGTTGGTGAAGTGCGGGTTGATTTGCAGCGGGAACAGATTTAGCGCATCGAAACCTTGCGGATCGACAATCGGCATATCGTTGGTGGTACGAATAGTTGGGCAAGCAAGGTTAGCGCCTGCGCTCCAGCCAATATACAGAGCGCCACGTTTAACCACGTCAGTAATTGGTGCCAGCAGCCCGCGCTCGCGGCACTGTTTCAGCAACTGGAAAGTATTCCCGCCGCCGACAATCACGA > NC_000913/4229630‑4229898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |