Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,285,518	A→G	I618I (ATT→ATC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,285,518	0	A	G	100.0%	220.3 / NA	58	I618I (ATT→ATC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/23);  total (35/23)

CGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTC  >  NC_000913/4285383‑4285652                                                                                                                                        |                                                                                                                                       cGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGAtt                                                                                                                                     >  2:1358870/1‑139 (MQ=255)         aCTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAg                                                                                                                             >  2:1549505/1‑139 (MQ=255)             gCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATa                                                                                                                         <  2:654004/139‑1 (MQ=255)              cATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTGTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATAc                                                                                                                        >  1:1265404/1‑139 (MQ=255)                   ggCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGgcgc                                                                                                                   >  1:423497/1‑139 (MQ=255)                         tgtgGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAAtt                                                                                                             >  1:242608/1‑139 (MQ=255)                                tACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCgg                                                                                                      >  2:1240160/1‑139 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    >  2:1221137/1‑139 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    >  1:1565466/1‑139 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    >  1:1075068/1‑139 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    >  1:514072/1‑139 (MQ=255)                                  cGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    <  1:47890/139‑1 (MQ=255)                                     tGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCgg                                                                                                 >  1:1109339/1‑139 (MQ=255)                                          tCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    <  1:1782599/131‑1 (MQ=255)                                          tCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAg                                                                                                    >  2:1782599/1‑131 (MQ=255)                                             cgATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAgg                                                                                         >  2:619519/1‑139 (MQ=255)                                                tAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAg                                                                                      <  2:423497/139‑1 (MQ=255)                                                            ctTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGGCCa                                                                          >  1:614277/1‑139 (MQ=255)                                                             ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAg                                                                         <  2:242608/139‑1 (MQ=255)                                                             ttCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAg                                                                         >  1:372876/1‑139 (MQ=255)                                                                     gcTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCAc                                                                 >  2:204890/1‑139 (MQ=255)                                                                      cTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACg                                                                >  2:895092/1‑139 (MQ=255)                                                                       ttCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGt                                                               >  1:570512/1‑139 (MQ=255)                                                                       ttCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGt                                                               >  2:504170/1‑139 (MQ=255)                                                                            gACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTa                                                                                           >  1:412463/1‑106 (MQ=255)                                                                            gACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTa                                                                                           <  2:412463/106‑1 (MQ=255)                                                                            gACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGc                                                          <  2:289178/139‑1 (MQ=255)                                                                                        aTCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCggg                                              <  1:1240160/139‑1 (MQ=255)                                                                                           ggCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCa                                           <  2:1075068/139‑1 (MQ=255)                                                                                           ggCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCa                                           <  1:1075500/139‑1 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgc                                                                                                                                 <  2:589906/52‑1 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTgc                                                                                                                                 >  1:589906/1‑52 (MQ=255)                                                                                            gCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCaa                                          <  2:1265404/139‑1 (MQ=255)                                                                                                 cGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATctct                                     >  1:639504/1‑139 (MQ=255)                                                                                                     gAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTAc                                 <  2:553145/139‑1 (MQ=255)                                                                                                     gAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTAc                                 >  1:601300/1‑139 (MQ=255)                                                                                                          aTCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                  <  2:79314/133‑1 (MQ=255)                                                                                                          aTCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTa                                  >  1:79314/1‑133 (MQ=255)                                                                                                          aTCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                            <  2:1565466/139‑1 (MQ=255)                                                                                                          aTCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                            <  2:1109339/139‑1 (MQ=255)                                                                                                          aTCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                            <  1:204890/139‑1 (MQ=255)                                                                                                          aTCGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                            <  2:570512/139‑1 (MQ=255)                                                                                                            cGCCCAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCa                          >  1:771307/1‑139 (MQ=255)                                                                                                              cccAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGtt                                                                                              <  2:1268112/69‑1 (MQ=255)                                                                                                              cccAGGTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGtt                                                                                              >  1:1268112/1‑69 (MQ=255)                                                                                                              cccAGGTTGCTGGTATCGCCCGCCGCGATTTTACGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGt                        >  2:1647189/1‑139 (MQ=255)                                                                                                                   gTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                            <  2:844789/130‑1 (MQ=255)                                                                                                                   gTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCAcc                            >  1:844789/1‑130 (MQ=255)                                                                                                                   gTTGCTGGTATCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCgg                   >  1:982484/1‑139 (MQ=255)                                                                                                                            aTCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCg          <  1:1221137/139‑1 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                  <  1:399563/130‑1 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGa                  >  2:399563/1‑130 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCt             >  2:995809/1‑135 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCt             <  1:995809/135‑1 (MQ=255)                                                                                                                             tCGCCCGCCGCGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGt         >  1:806007/1‑139 (MQ=255)                                                                                                                                    ccgcGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTc  >  1:973356/1‑139 (MQ=255)                                                                                                                                    ccgcGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTc  >  1:70221/1‑139 (MQ=255)                                                                                                                                    ccgcGATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTc  >  1:1719182/1‑139 (MQ=255)                                                                                                                                     cgcgATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGTGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGc                                             >  1:887217/1‑95 (MQ=255)                                                                                                                                        |                                                                                                                                       CGTTACCGACTCGCATCGGGCAATTGTGGGTTACGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCAGCTTCTCGACTACGCCAGGATCGGCAAGCGTCGAGGTATCGCCCAGGTTGCTGGTATCGCCCGCCGCAATTTTGCGCAGAATACGGCGCATAATTTTGCCGGAGCGGGTTTTAGGCAGGGAGTCGGTCCAGTGCAGCACGTCTGGCGTCGCCAGCGGGCCAATCTCTTTACGCACCCAGTTGCGGACTTCTGCGTACAGTTC  >  NC_000913/4285383‑4285652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑