| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,392,496 | C→T | noncoding (25/76 nt) | glyX → | tRNA‑Gly |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,392,496 | 0 | C | T | 98.0% | 163.6 / NA | 51 | noncoding (25/76 nt) | glyX | tRNA‑Gly |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (25/25); total (25/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCG > NC_000913/4392367‑4392625 | tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTg < 1:1354119/139‑1 (MQ=255)tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTg > 2:1354119/1‑139 (MQ=255) gCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGcc < 1:22583/139‑1 (MQ=255) agagCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTGGGTAGAGTACGACCTTGCCAAGGTCGGGGt > 1:1168833/1‑139 (MQ=255) gCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTcgc > 2:1149115/1‑139 (MQ=255) cACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTcgcg < 2:331021/139‑1 (MQ=255) aCCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACC‑TGCCAAGGTCGGGGTCGCGAGTTc > 2:1761888/1‑139 (MQ=255) tGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAg > 1:331021/1‑139 (MQ=255) gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt > 2:1106591/1‑139 (MQ=255) gTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGtt > 2:775322/1‑139 (MQ=255) gggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTccc > 1:1705230/1‑139 (MQ=255) ggTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCg > 1:739192/1‑139 (MQ=255) ttCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACC‑TGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAAtt < 1:1761888/139‑1 (MQ=255) cGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg < 1:1106591/139‑1 (MQ=255) tttCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCt > 1:353891/1‑139 (MQ=255) tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt < 2:924994/139‑1 (MQ=255) tCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt < 2:353891/139‑1 (MQ=255) aTTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGacc < 1:1149115/139‑1 (MQ=255) tGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGaccacc < 1:18609/139‑1 (MQ=255) gAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCgggg > 1:952597/1‑76 (MQ=255) gAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCgggg < 2:952597/76‑1 (MQ=255) aaGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAg < 2:739192/139‑1 (MQ=255) tgctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGt < 1:508503/65‑1 (MQ=255) tgctgcAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGt > 2:508503/1‑65 (MQ=255) cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATa < 2:963577/139‑1 (MQ=255) cAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATa > 1:573770/1‑139 (MQ=255) cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg > 2:875238/1‑103 (MQ=255) cacaGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg < 1:875238/103‑1 (MQ=255) caGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGt < 2:573770/139‑1 (MQ=255) aaccaccAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTgg < 1:1341422/134‑1 (MQ=255) ccAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg < 1:1307569/93‑1 (MQ=255) ccAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg > 2:1307569/1‑93 (MQ=255) ccAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTagag > 1:158492/1‑139 (MQ=255) ccAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGCCACAGACTACCCAAGCGGGAATAGCTCAGTTGGTagag > 1:862066/1‑139 (MQ=255) ccAAGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGCGATGTAAGGCCCACACCACCCAGGCGGGAAAAGGTCAGTTGGTagag > 1:356187/1‑139 (MQ=14) ccAAGCGGGAATAGCTCAGTTGGT‑GAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGc > 2:216560/1‑139 (MQ=255) aaGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCa < 2:158492/139‑1 (MQ=255) aaGCGGGAATAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCa < 2:1705230/139‑1 (MQ=255) cGGGAATAGCTCAGTTGGTAGAGTACAACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGa > 2:328097/1‑139 (MQ=255) gggAATAGCTCAGTTGGTAGAGTACAACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGAc < 1:328097/139‑1 (MQ=255) aaTAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTa < 1:640796/89‑1 (MQ=255) aaTAGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTa > 2:640796/1‑89 (MQ=255) aGCTCAGTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc < 2:900736/139‑1 (MQ=255) aGCTCAGTTGGCAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTGCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc < 2:1168833/139‑1 (MQ=255) gTTGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc > 1:900736/1‑139 (MQ=255) ttGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt < 1:885516/78‑1 (MQ=255) ttGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGt > 2:885516/1‑78 (MQ=255) ttGGTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCg > 2:1055602/1‑139 (MQ=255) gTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg > 2:517760/1‑69 (MQ=14) gTAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAg < 1:517760/69‑1 (MQ=14) tAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg > 1:1534615/1‑70 (MQ=255) tAGAGTACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTg < 2:1534615/70‑1 (MQ=255) | TAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAAGTGCTGCAAAGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAAAATTTGAAAGTGCTGTAAGGCACAGACCACCCAAGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCG > NC_000913/4392367‑4392625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |