Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,043,741 A→G V157A (GTT→GCT)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,043,7410AG100.0% 347.4 / NA 91V157A (GTT→GCT) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/44);  total (47/44)

GGACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAACCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGC  >  NC_000913/1043607‑1043869
                                                                                                                                      |                                                                                                                                
ggACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGt                                                                                                                              >  2:190822/1‑139 (MQ=255)
 gACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTg                                                                                                                             >  1:482048/1‑139 (MQ=255)
 gACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTg                                                                                                                             >  1:1408994/1‑139 (MQ=255)
      aCTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAgtgt                                                                                                                        >  1:16797/1‑139 (MQ=255)
        tgtgTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                      <  2:74225/139‑1 (MQ=255)
        tgtgTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                      <  1:320421/139‑1 (MQ=255)
           gtTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCg                                                                                                                               <  1:1448655/127‑1 (MQ=255)
           gtTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCg                                                                                                                               >  2:1448655/1‑127 (MQ=255)
            ttCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGTGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTg                                                                                                                  <  1:1495251/139‑1 (MQ=255)
             tCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGc                                                                                                                 <  2:482048/139‑1 (MQ=255)
                    ttGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCt                                                                                                          <  2:445379/139‑1 (MQ=255)
                    ttGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCt                                                                                                          <  1:748502/139‑1 (MQ=255)
                      ggCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGa                                                                                                        >  2:244209/1‑139 (MQ=255)
                           tAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   <  1:244209/139‑1 (MQ=255)
                           tAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   <  2:881953/139‑1 (MQ=255)
                            aaTGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTc                                                                                                  >  1:1342568/1‑139 (MQ=255)
                            aaTGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTc                                                                                                  >  2:1394904/1‑139 (MQ=255)
                            aaTGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTc                                                                                                  >  1:1060126/1‑139 (MQ=255)
                              tGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAg                                                                                                <  2:1342568/139‑1 (MQ=255)
                                  cgcgATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGcc                                                                                                                                <  2:138818/103‑1 (MQ=255)
                                  cgcgATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTc                                                                                                                       <  2:1528720/112‑1 (MQ=255)
                                  cgcgATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTc                                                                                                                       >  1:1528720/1‑112 (MQ=255)
                                  cgcgATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTtgt                                                                                            >  1:851063/1‑139 (MQ=255)
                                    cgATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTgg                                                                                          <  2:1607897/139‑1 (MQ=255)
                                      aTAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGaa                                                                                        <  1:172655/139‑1 (MQ=255)
                                       tAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAat                                                                                       <  2:1248455/139‑1 (MQ=255)
                                       tAGTCAGCCCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAat                                                                                       <  2:1060126/139‑1 (MQ=255)
                                            aGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCa                                                                                  <  2:411403/139‑1 (MQ=255)
                                                             ttCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAg                                                                  >  2:327758/1‑138 (MQ=255)
                                                             ttCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAg                                                                  <  1:327758/138‑1 (MQ=255)
                                                                 aGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAc                                                             <  2:851063/139‑1 (MQ=255)
                                                                  gACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAcc                                                            <  1:190822/139‑1 (MQ=255)
                                                                  gACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAcc                                                            <  2:16797/139‑1 (MQ=255)
                                                                         gCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGtttt                                                     >  2:175751/1‑139 (MQ=255)
                                                                            gACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCt                                                  <  2:1663174/139‑1 (MQ=255)
                                                                              ccataccatTGACGGTGAACTCTTCACCTTCCAGAGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCttt                                                >  1:1418440/1‑139 (MQ=255)
                                                                                             tGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCa                                 >  2:478117/1‑139 (MQ=255)
                                                                                               aaCTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGc                               <  1:286705/139‑1 (MQ=255)
                                                                                               aaCTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGc                               >  2:1383871/1‑139 (MQ=255)
                                                                                                 ctctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCcg                             <  1:1383871/139‑1 (MQ=255)
                                                                                                  tctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   <  1:210530/68‑1 (MQ=255)
                                                                                                  tctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   >  2:210530/1‑68 (MQ=255)
                                                                                                   ctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCcgcg                           >  1:1170898/1‑139 (MQ=255)
                                                                                                   ctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCcgcg                           <  1:412330/139‑1 (MQ=255)
                                                                                                   ctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCcgcg                           >  2:405193/1‑139 (MQ=255)
                                                                                                    ttCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                      >  1:811305/1‑47 (MQ=255)
                                                                                                    ttCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                      <  2:811305/47‑1 (MQ=255)
                                                                                                    ttCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGa                          >  1:1498740/1‑139 (MQ=255)
                                                                                                    ttCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGAGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGcc                                   >  2:1587460/1‑130 (MQ=255)
                                                                                                    ttCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGAGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGcc                                   <  1:1587460/130‑1 (MQ=255)
                                                                                                     tCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGa                                                                                                                            <  1:1001066/40‑1 (MQ=255)
                                                                                                     tCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGa                                                                                                                            >  2:1001066/1‑40 (MQ=39)
                                                                                                           tCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                      >  2:81154/1‑40 (MQ=39)
                                                                                                           tCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                      <  1:81154/40‑1 (MQ=255)
                                                                                                            ccAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAAt                  <  2:592870/139‑1 (MQ=255)
                                                                                                              aGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTg                                                                                                                  >  1:1431423/1‑41 (MQ=255)
                                                                                                              aGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTg                                                                                                                  <  2:1431423/41‑1 (MQ=255)
                                                                                                              aGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATcc                                                                               <  2:538967/76‑1 (MQ=255)
                                                                                                              aGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATcc                                                                               >  1:538967/1‑76 (MQ=255)
                                                                                                              aGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCg                >  2:431772/1‑139 (MQ=255)
                                                                                                                     aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   >  1:74448/1‑49 (MQ=255)
                                                                                                                     aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   <  2:74448/49‑1 (MQ=255)
                                                                                                                     aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATa         >  1:1489628/1‑139 (MQ=255)
                                                                                                                     aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATa         >  1:1436466/1‑139 (MQ=255)
                                                                                                                     aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATa         >  1:1012955/1‑139 (MQ=255)
                                                                                                                       tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   >  2:398776/1‑47 (MQ=255)
                                                                                                                       tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                   <  1:398776/47‑1 (MQ=255)
                                                                                                                       tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACt       <  1:1449969/139‑1 (MQ=255)
                                                                                                                       tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACt       >  1:249075/1‑139 (MQ=255)
                                                                                                                       tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACt       >  1:331795/1‑139 (MQ=255)
                                                                                                                       tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACt       >  1:1575746/1‑139 (MQ=255)
                                                                                                                        ggCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCg                <  2:635590/129‑1 (MQ=255)
                                                                                                                        ggCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCg                >  1:635590/1‑129 (MQ=255)
                                                                                                                        ggCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACtt      <  2:1418440/139‑1 (MQ=255)
                                                                                                                         gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCa                                                                                                     <  2:1538718/43‑1 (MQ=255)
                                                                                                                         gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCa                                                                                                     >  1:1538718/1‑43 (MQ=255)
                                                                                                                         gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTc     >  2:685333/1‑139 (MQ=255)
                                                                                                                          ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGc                                                                 <  2:1024452/78‑1 (MQ=255)
                                                                                                                          ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCt    >  1:1766854/1‑139 (MQ=255)
                                                                                                                          ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCt    >  2:12630/1‑139 (MQ=255)
                                                                                                                          ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCt    >  1:1457911/1‑139 (MQ=255)
                                                                                                                          ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATAGCCAGc                                                                 >  1:1024452/1‑78 (MQ=255)
                                                                                                                           cGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTg   <  1:478117/139‑1 (MQ=255)
                                                                                                                           cGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTg   <  2:249075/139‑1 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCGTCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGc  >  1:1668677/1‑139 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAc                                                             <  1:542590/80‑1 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAc                                                             >  2:542590/1‑80 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACaa                   <  1:752061/122‑1 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACaa                   >  2:752061/1‑122 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGc  >  2:1508482/1‑139 (MQ=255)
                                                                                                                            gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGc  >  2:1606436/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                
GGACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAACCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGC  >  NC_000913/1043607‑1043869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑