Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,544,910 G→A V203M (GTG→ATG)  fimC → periplasmic chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,544,9100GA100.0% 364.6 / NA 105V203M (GTG→ATG) fimCperiplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (53/52);  total (53/52)

Only 100 of 105 total aligned reads displayed.

GCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAAT  >  NC_000913/4544777‑4545022
                                                                                                                                     |                                                                                                                
gCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCt                                                                                                             >  2:1334551/1‑139 (MQ=255)
gCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCt                                                                                                             >  1:1294836/1‑139 (MQ=255)
        aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATggg                                                                                                     >  2:1378292/1‑139 (MQ=255)
              ccGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAg                                                                                               <  1:519238/139‑1 (MQ=255)
              ccGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAg                                                                                               <  2:1297342/139‑1 (MQ=255)
                gCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCa                                                                                             <  1:928790/139‑1 (MQ=255)
                 cAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCAc                                                                                            >  1:33544/1‑139 (MQ=255)
                  aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACg                                                                                           >  2:318900/1‑139 (MQ=255)
                  aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACg                                                                                           >  1:695637/1‑139 (MQ=255)
                  aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACg                                                                                           >  1:791867/1‑139 (MQ=255)
                     aaaaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGtt                                                                                        <  2:204667/139‑1 (MQ=255)
                               tttCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCtt                                                                              <  1:797450/139‑1 (MQ=255)
                               tttCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCtt                                                                              <  2:1294836/139‑1 (MQ=255)
                                ttCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTc                                                                             >  1:479974/1‑139 (MQ=255)
                                       tAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCa                                                                      <  1:1378292/139‑1 (MQ=255)
                                          cgcgAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                   <  1:1334551/139‑1 (MQ=255)
                                            cgAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                 <  1:798206/139‑1 (MQ=255)
                                              aaTTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               <  1:1667129/139‑1 (MQ=255)
                                              aaTTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               <  1:318900/139‑1 (MQ=255)
                                                     tGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAgg                                                                    >  2:560826/1‑127 (MQ=255)
                                                       aCGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATAttactt                                                      >  1:134330/1‑139 (MQ=255)
                                                           tGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCg                                                  <  2:791867/139‑1 (MQ=255)
                                                           tGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCg                                                  <  2:479974/139‑1 (MQ=255)
                                                            gATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGa                                                 >  2:1679084/1‑139 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAATCACTGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               >  2:1608839/1‑124 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               <  1:9080/124‑1 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               <  1:1608839/124‑1 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               >  2:9080/1‑124 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                <  1:856200/139‑1 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                <  2:1277824/139‑1 (MQ=255)
                                                             aTTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                <  2:33544/139‑1 (MQ=255)
                                                              ttAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAAc                                               <  2:134330/139‑1 (MQ=255)
                                                                 aCCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCAAACAAt                                            >  1:912499/1‑139 (MQ=255)
                                                                   ccGACACCCTATTACCTGACGGTAACAGAGTTGAATGACGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATaa                                          <  2:697718/139‑1 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCaa                                                                                                         <  2:1690657/72‑1 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCaa                                                                                                         >  1:1690657/1‑72 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAGTGGGCGAAAGCACGGtt                                                                                        <  2:101845/89‑1 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAGTGGGCGAAAGCACGGtt                                                                                        >  1:101845/1‑89 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTaaa                                                                                     <  2:686943/92‑1 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTaaa                                                                                     >  1:686943/1‑92 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGa                                      >  2:579005/1‑139 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGa                                      <  2:531564/139‑1 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGa                                      >  1:531564/1‑139 (MQ=255)
                                                                       cacCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGa                                      >  2:145737/1‑139 (MQ=255)
                                                                            tATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               >  2:1706680/1‑109 (MQ=255)
                                                                            tATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCa                                                               <  1:1706680/109‑1 (MQ=255)
                                                                                 ccTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                 >  1:1362932/1‑102 (MQ=255)
                                                                                 ccTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                 <  2:1362932/102‑1 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGa                                                                                                  <  2:1265656/66‑1 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCa                                                                                             >  1:1301293/1‑71 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCa                                                                                             <  2:1301293/71‑1 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc                                                                                <  1:1201338/84‑1 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGgcg                              <  1:1622574/134‑1 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGgcg                              >  2:1622574/1‑134 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACtt                         >  1:1214750/1‑139 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACtt                         >  2:1039450/1‑139 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACtt                         >  1:1299654/1‑139 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACtt                         >  1:1391800/1‑139 (MQ=255)
                                                                                    gACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGAATTGATGCCTCCAATGGGCGa                                                                                                  >  1:1265656/1‑66 (MQ=255)
                                                                                    gACGCTAACAGAGGTGAATGCCGGAACCCGGGCTCTTGAAAATGCATTGATGCCGCCATTGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGTACtt                         >  1:407559/1‑139 (MQ=255)
                                                                                     aCGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                   >  2:1113062/1‑96 (MQ=255)
                                                                                     aCGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                   <  1:1113062/96‑1 (MQ=255)
                                                                                     aCGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                 >  1:353973/1‑98 (MQ=255)
                                                                                     aCGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAg                                                                 <  2:353973/98‑1 (MQ=255)
                                                                                     aCGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTa                        >  2:26614/1‑139 (MQ=255)
                                                                                        gTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTAccc                     <  2:1214750/139‑1 (MQ=255)
                                                                                               agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCg                                                  >  2:1778124/1‑103 (MQ=255)
                                                                                               agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCg                                                  <  1:1778124/103‑1 (MQ=255)
                                                                                               agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATa                                           <  1:156445/110‑1 (MQ=255)
                                                                                               agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATa                                           >  2:156445/1‑110 (MQ=255)
                                                                                               agTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAAt              >  1:450066/1‑139 (MQ=255)
                                                                                                 ttGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGa            >  1:1049286/1‑139 (MQ=255)
                                                                                                 ttGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTAAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCGCAAAATGa            >  2:1060920/1‑139 (MQ=255)
                                                                                                   gAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCa                                                                                             <  1:310899/56‑1 (MQ=255)
                                                                                                   gAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCa                                                                                             >  2:310899/1‑56 (MQ=255)
                                                                                                   gAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACg          >  2:1059999/1‑139 (MQ=255)
                                                                                                   gAATGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACg          >  1:1379168/1‑139 (MQ=255)
                                                                                                    aaTGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACgg         >  1:891470/1‑139 (MQ=255)
                                                                                                     aTGCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGGAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTAACCCCAAAATGACggg        <  1:579005/139‑1 (MQ=255)
                                                                                                       gCCGGAACCCGGGTTCTTGAAGATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCg      <  1:1039450/139‑1 (MQ=255)
                                                                                                       gCCGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCg      <  1:1059999/139‑1 (MQ=255)
                                                                                                        ccGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGt     <  1:1679084/139‑1 (MQ=255)
                                                                                                         cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCTAATGGGCGAAAGCACGGTTAAATTGcc                                                                                <  1:1066268/63‑1 (MQ=255)
                                                                                                         cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc                                                                                >  2:1066268/1‑63 (MQ=255)
                                                                                                         cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg        <  2:1751265/135‑1 (MQ=255)
                                                                                                         cGGAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg        >  1:1751265/1‑135 (MQ=255)
                                                                                                          ggAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTaa   <  2:800390/139‑1 (MQ=255)
                                                                                                          ggAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTaa   <  2:450066/139‑1 (MQ=255)
                                                                                                           gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                   <  1:1194819/74‑1 (MQ=255)
                                                                                                           gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAAt  >  2:245332/1‑139 (MQ=255)
                                                                                                           gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAAt  >  2:1470314/1‑139 (MQ=255)
                                                                                                           gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAAt  <  2:1049286/139‑1 (MQ=255)
                                                                                                           gAACCCGGGTTCTTGAAAATGCATTGATGCCTCCAATCGGCGAGAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                   >  2:1194819/1‑74 (MQ=255)
                                                                                                              cccGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCa                                                                      <  1:552037/68‑1 (MQ=255)
                                                                                                              cccGGGTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCa                                                                      >  2:552037/1‑68 (MQ=255)
                                                                                                                  ggTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg        <  1:805729/126‑1 (MQ=255)
                                                                                                                  ggTTCTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACggg        >  2:805729/1‑126 (MQ=255)
                                                                                                                      cTTGAAAATGCATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGa                                                                   <  1:1761237/63‑1 (MQ=255)
                                                                                                                                cATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                >  1:1250862/1‑72 (MQ=255)
                                                                                                                                cATTGATGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGaa                                                <  2:1250862/72‑1 (MQ=255)
                                                                                                                                     |                                                                                                                
GCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGATGCAGGAAGCAATATTACTTACCGAACAATAAATGATTATGGCGCACTTACCCCCAAAATGACGGGCGTAAT  >  NC_000913/4544777‑4545022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: