Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,379,266 (C)7→8 coding (732/1128 nt) zapE ← divisome ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,379,2591.C95.4% 299.4 / 5.8 87coding (739/1128 nt)zapEdivisome ATPase
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base C (39/44);  total (41/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTT  >  NC_000913/3379121‑3379382
                                                                                                                                            |                                                                                                                            
gTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt                                                                                                                               <  1:818742/139‑1 (MQ=255)
gTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGttt                                                                                                                               <  2:305174/139‑1 (MQ=255)
                gTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              <  2:353878/139‑1 (MQ=255)
                    tGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGACGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAAc                                                                                                          >  2:367101/1‑139 (MQ=255)
                     gCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAAcc                                                                                                         <  1:346129/139‑1 (MQ=255)
                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              >  2:1398718/1‑133 (MQ=255)
                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              <  1:1398718/133‑1 (MQ=255)
                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCa                                                                                                        >  1:1089885/1‑139 (MQ=255)
                      cTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCa                                                                                                        >  2:1513075/1‑139 (MQ=255)
                          cTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGt                                                                                                    <  1:972930/139‑1 (MQ=255)
                                   tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAAcc                                                                                                         >  1:68330/1‑124 (MQ=255)
                                   tCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAAcc                                                                                                         <  2:68330/124‑1 (MQ=255)
                                     gACGCACAGCGTAGTAAAAGAGACAGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTg                                                                                         >  2:282448/1‑139 (MQ=255)
                                        gCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                                                     <  2:924046/139‑1 (MQ=255)
                                         cacaGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGcc                                                                                     <  2:1089885/139‑1 (MQ=255)
                                              cGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGt                                                                                >  2:632284/1‑139 (MQ=255)
                                                  gtaAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCa                                                                            <  1:632284/139‑1 (MQ=255)
                                                      aaGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGt                                                                        >  2:310878/1‑139 (MQ=255)
                                                        gagaCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGa                                                                      >  2:104048/1‑139 (MQ=255)
                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTCTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                                                   >  1:1007627/1‑139 (MQ=255)
                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      <  2:1053954/120‑1 (MQ=255)
                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      >  1:1053954/1‑120 (MQ=255)
                                                           aCCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGt                                                                   >  2:1373035/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:645824/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:791252/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:1405225/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  1:215427/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  2:1088742/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTgg                                                                 >  1:1321361/1‑139 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTccccc                                                                                                                        <  2:405276/83‑1 (MQ=255)
                                                             cGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTccccc                                                                                                                        >  1:405276/1‑83 (MQ=255)
                                                             cGCCAGCGTCTGGCTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcg                                                                                                               >  2:138473/1‑93 (MQ=255)
                                                             cGCCAGCGTCTGGCTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcg                                                                                                               <  1:138473/93‑1 (MQ=255)
                                                               ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               <  1:791252/139‑1 (MQ=255)
                                                               ccAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               <  2:400993/139‑1 (MQ=255)
                                                                cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                                              <  1:367101/139‑1 (MQ=255)
                                                                cAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGa                                                              <  1:1088742/139‑1 (MQ=255)
                                                                 aGCGTCTGTTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCTCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                                             <  1:896431/139‑1 (MQ=255)
                                                                 aGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGaa                                                             <  1:104048/139‑1 (MQ=255)
                                                                            ctcGACGCCCATTTTTGCTAATGGCCGATGGTTGAGTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATg                                                  <  2:722200/139‑1 (MQ=255)
                                                                             tcGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgc                                                 <  2:522142/139‑1 (MQ=255)
                                                                                aCGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGcc                                              >  2:1445099/1‑139 (MQ=255)
                                                                                    ccAGTGTTGCTAATGGCCGATGGTTAATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag                                          <  1:973869/139‑1 (MQ=255)
                                                                                     cATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt                                         <  1:272919/139‑1 (MQ=255)
                                                                                        tgttgCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagtgag                                      <  1:1445099/139‑1 (MQ=255)
                                                                                             cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCa                                                                                                        <  2:1027692/68‑1 (MQ=255)
                                                                                             cTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCa                                                                                                        >  1:1027692/1‑68 (MQ=255)
                                                                                              tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              >  1:1173724/1‑61 (MQ=255)
                                                                                              tAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              <  2:1173724/61‑1 (MQ=255)
                                                                                                 tGGCCGATGGTTGATTTATAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAGCCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTCGCGAAAGCCACAGATCCGCCTGAGTGAGTGTACGCAg                             >  2:1035745/1‑139 (MQ=255)
                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      <  1:169224/81‑1 (MQ=255)
                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      >  2:169224/1‑81 (MQ=255)
                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               >  1:689519/1‑120 (MQ=255)
                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               <  2:689519/120‑1 (MQ=255)
                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag                                          <  2:689943/125‑1 (MQ=255)
                                                                                                  ggCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgag                                          >  1:689943/1‑125 (MQ=255)
                                                                                                    ccGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACg                          <  1:1373035/139‑1 (MQ=255)
                                                                                                     cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      <  1:56432/78‑1 (MQ=255)
                                                                                                     cGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTgcgc                                                                                      >  2:56432/1‑78 (MQ=255)
                                                                                                       aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAAcc                                                                                                         <  2:20960/57‑1 (MQ=255)
                                                                                                       aTGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAAcc                                                                                                         >  1:20960/1‑57 (MQ=255)
                                                                                                          gTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt                                         <  2:1486714/118‑1 (MQ=255)
                                                                                                          gTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCtgagt                                         >  1:1486714/1‑118 (MQ=255)
                                                                                                          gTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATc                    <  2:907174/139‑1 (MQ=255)
                                                                                                           ttGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               >  2:1189442/1‑111 (MQ=255)
                                                                                                           ttGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATgcgc                                               <  1:1189442/111‑1 (MQ=255)
                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              >  1:1141941/1‑46 (MQ=255)
                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAgcgc                                                                                                              <  2:1141941/46‑1 (MQ=255)
                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGcc                                                                                                             <  1:258239/47‑1 (MQ=39)
                                                                                                             gATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGcc                                                                                                             >  2:258239/1‑47 (MQ=39)
                                                                                                                  cTAACGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAg            <  1:380056/139‑1 (MQ=255)
                                                                                                                     aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTAt                                                                                                >  1:1212974/1‑52 (MQ=255)
                                                                                                                     aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTAt                                                                                                <  2:1212974/52‑1 (MQ=255)
                                                                                                                     aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                                               >  2:1097633/1‑69 (MQ=255)
                                                                                                                     aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGtt                                                                               <  1:1097633/69‑1 (MQ=255)
                                                                                                                     aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                                                   >  2:1460457/1‑97 (MQ=255)
                                                                                                                     aCGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGAt                                                   <  1:1460457/97‑1 (MQ=255)
                                                                                                                      cGTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTc        <  1:1465345/139‑1 (MQ=255)
                                                                                                                       gTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc       <  2:215427/139‑1 (MQ=255)
                                                                                                                       gTCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTcc       <  2:1321361/139‑1 (MQ=255)
                                                                                                                        tCGGTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCa      >  2:1016567/1‑139 (MQ=255)
                                                                                                                          ggTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               <  1:119093/80‑1 (MQ=255)
                                                                                                                          ggTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCg                                                               >  2:119093/1‑80 (MQ=255)
                                                                                                                          ggTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                                                         >  2:231958/1‑86 (MQ=255)
                                                                                                                          ggTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGcc                                                         <  1:231958/86‑1 (MQ=255)
                                                                                                                          ggTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg    >  1:1404508/1‑139 (MQ=255)
                                                                                                                          ggTGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACg    >  1:199443/1‑139 (MQ=255)
                                                                                                                            tGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  <  2:199443/139‑1 (MQ=255)
                                                                                                                            tGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  <  1:282448/139‑1 (MQ=255)
                                                                                                                            tGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  <  2:137540/139‑1 (MQ=255)
                                                                                                                            tGAATTTTCTCGTTTCCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGtt  >  1:571431/1‑139 (MQ=255)
                                                                                                                                            |                                                                                                                            
GTGAGAGCGCAATATAGTCATGCTGACTGCGGGCGTCGACGCACAGCGTAGTAAAAGAGACCGCCAGCGTCTGGTTCTCGACGCCCATTGTTGCTAATGGCCGATGGTTGATTTCTAACGTCGGTGAATTTTCTCGTTTCCCCCCCGCCAGCGCCAACCATAGTTTATCCATTTGCGCCCGGGTTTCATCGTGAAGTGGCGAAAGCCACAGATGCGCCTGAGTGAGTGTACGCAGACGATAATCAACACCAGCGTCCACGTT  >  NC_000913/3379121‑3379382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: