Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 254,408 A→G F437L (TTC→CTC)  pepD ← aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913254,4080AG100.0% 212.7 / NA 59F437L (TTC→CTC) pepDaminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/28);  total (31/28)

ATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCG  >  NC_000913/254272‑254542
                                                                                                                                        |                                                                                                                                      
aTTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCa                                                                                                                                      <  1:528702/139‑1 (MQ=255)
     tttcAGCAGTTCAGTCAGCAGGGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACca                                                                                                                                 <  1:890898/139‑1 (MQ=255)
              ttCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAgg                                                                                                                        <  1:557636/139‑1 (MQ=255)
                        agTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGa                                                                                                              <  1:537083/139‑1 (MQ=255)
                            tCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAgg                                                                                                                        <  1:1215034/125‑1 (MQ=255)
                            tCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAgg                                                                                                                        >  2:1215034/1‑125 (MQ=255)
                            tCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAAt                                                                                                          >  1:749140/1‑139 (MQ=255)
                                     gACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGtt                                                                                                 <  2:380179/139‑1 (MQ=255)
                                      aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGATTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTc                                                                                                >  1:224190/1‑139 (MQ=255)
                                      aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACca                                                                                                                                 <  1:340671/106‑1 (MQ=255)
                                      aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACca                                                                                                                                 >  2:340671/1‑106 (MQ=255)
                                      aCCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTc                                                                                                >  1:561964/1‑139 (MQ=255)
                                          aCGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCg                                                                                            <  2:431455/139‑1 (MQ=255)
                                                tCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCttgt                                                                                      >  1:635096/1‑139 (MQ=255)
                                                     gtgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAAc                                                                                 >  1:227740/1‑139 (MQ=255)
                                                      tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACa                                                                                >  1:238294/1‑139 (MQ=255)
                                                      tgAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACa                                                                                >  1:751134/1‑139 (MQ=255)
                                                        aaCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                                                                              >  2:681117/1‑139 (MQ=255)
                                                        aaCTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAgg                                                                              <  2:749140/139‑1 (MQ=255)
                                                                  tCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                          <  1:255923/117‑1 (MQ=255)
                                                                  tCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTc                                                                                          >  2:255923/1‑117 (MQ=255)
                                                                    cGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGtt                                                                  <  2:227740/139‑1 (MQ=255)
                                                                     ggAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGttt                                                                 <  1:1381806/139‑1 (MQ=255)
                                                                             tgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAgg                                                                                                                        <  1:276185/76‑1 (MQ=255)
                                                                             tgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAgg                                                                                                                        >  2:276185/1‑76 (MQ=255)
                                                                             tgGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAcc                                                         >  2:306876/1‑139 (MQ=255)
                                                                                    gTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCa                                                  <  1:306876/139‑1 (MQ=255)
                                                                                     tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGa                                                                                                              >  2:1491294/1‑78 (MQ=255)
                                                                                     tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGa                                                                                                              <  1:1491294/78‑1 (MQ=255)
                                                                                     tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt                                                 >  1:1463484/1‑139 (MQ=255)
                                                                                     tGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCAt                                                 >  2:86682/1‑139 (MQ=255)
                                                                                          gTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTgg                                                                                                               >  1:111883/1‑72 (MQ=255)
                                                                                          gTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTgg                                                                                                               <  2:111883/72‑1 (MQ=255)
                                                                                                   aTAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAg                                   >  2:1037685/1‑139 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGtt                                                                                                 <  2:322890/66‑1 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGtt                                                                                                 >  1:322890/1‑66 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACg                                                             >  1:160959/1‑102 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACg                                                             <  2:160959/102‑1 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                        >  1:1233691/1‑139 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                        >  1:1475683/1‑139 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                        <  1:275830/139‑1 (MQ=255)
                                                                                                              gTCCATTTCCGGATACGGTTTTTTGAGCAGACCACAATCCAGGCCCGCGTGTATATGCTGGATGTTCGGCGGCTTGGTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc                        <  2:1280667/139‑1 (MQ=255)
                                                                                                                 cATTTCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgccag                     >  1:707389/1‑139 (MQ=255)
                                                                                                                   tttCCGGATACGGTTTTTTGAGTAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACa                                                                                <  2:985058/78‑1 (MQ=255)
                                                                                                                   tttCCGGATACGGTTTTTTGAGTAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACa                                                                                >  1:985058/1‑78 (MQ=255)
                                                                                                                    ttCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTgg                                                                                                               >  2:1268959/1‑46 (MQ=255)
                                                                                                                    ttCCGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTgg                                                                                                               <  1:1268959/46‑1 (MQ=255)
                                                                                                                       cGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAgg                                                                    >  1:519067/1‑86 (MQ=255)
                                                                                                                       cGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAgg                                                                    <  2:519067/86‑1 (MQ=255)
                                                                                                                       cGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                      <  1:514892/116‑1 (MQ=255)
                                                                                                                       cGGATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAAt                                      >  2:514892/1‑116 (MQ=255)
                                                                                                                         gATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAcc                                                         >  2:363198/1‑95 (MQ=255)
                                                                                                                         gATACGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAcc                                                         <  1:363198/95‑1 (MQ=255)
                                                                                                                             cGGTTTTTTGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATgcg         <  2:635096/139‑1 (MQ=255)
                                                                                                                                ttttttGAGCAGACCACATTCCAGGCCCGCGTGGa                                                                                                              <  2:1239306/35‑1 (MQ=255)
                                                                                                                                ttttttGAGCAGACCACATTCCAGGCCCGCGTGGa                                                                                                              >  1:1239306/1‑35 (MQ=255)
                                                                                                                                    ttGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGata             >  2:1127523/1‑128 (MQ=255)
                                                                                                                                    ttGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGata             <  1:1127523/128‑1 (MQ=255)
                                                                                                                                    ttGAGCAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCg  >  1:165990/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
ATTTCTTTCAGCAGTTCAGTCAGCAGTGTCCAGTAATGACCTACGCTTTCGATGTGAACTTGCTCATCCGGAGAGTGTGGACCGGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAGGATATGCGCCTTTCG  >  NC_000913/254272‑254542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑