Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 792,525 A→G L422L (TTG→CTG)  modF ← molybdate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913792,5250AG100.0% 148.2 / NA 40L422L (TTG→CTG) modFmolybdate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/20);  total (20/20)

GCAGGCGCATCTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT  >  NC_000913/792389‑792656
                                                                                                                                        |                                                                                                                                   
gcaggcGCATCTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCg                                                                                                                                   >  1:963526/1‑139 (MQ=255)
   ggcGCATCTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCg                                                                                                                                <  2:908667/139‑1 (MQ=255)
    gcgcATCTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCgg                                                                                                                               >  1:291741/1‑139 (MQ=255)
     cgcATCTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGa                                                                                                                              <  2:1466533/139‑1 (MQ=255)
          cTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGttt                                                                                                                         >  1:1326189/1‑139 (MQ=255)
              aGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCAcc                                                                                                                     >  1:463134/1‑139 (MQ=255)
                     tgCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTgcgc                                                                                                              >  2:1441007/1‑139 (MQ=255)
                                           tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGa                                                                                                           <  1:346754/120‑1 (MQ=255)
                                           tttCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGa                                                                                                           >  2:346754/1‑120 (MQ=255)
                                             tCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACgctgct                                                                                      <  2:1326189/139‑1 (MQ=255)
                                                ccTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTc                                                                                   >  1:890468/1‑139 (MQ=255)
                                                 cTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTcc                                                                                  <  2:1203204/139‑1 (MQ=255)
                                                           cAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGa                                                                        <  1:690092/139‑1 (MQ=255)
                                                              cacaTCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACgctgct                                                                                      <  2:1111554/122‑1 (MQ=255)
                                                              cacaTCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACgctgct                                                                                      >  1:1111554/1‑122 (MQ=255)
                                                                 aTCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTg                                                                                                                 <  2:297099/92‑1 (MQ=255)
                                                                 aTCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTg                                                                                                                 >  1:297099/1‑92 (MQ=255)
                                                                   caacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGTTGCTGTCCCCAGGAAAGACt                                                                      <  2:885704/133‑1 (MQ=255)
                                                                   caacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTCTCCCCAGGAAAGACt                                                                      >  1:885704/1‑133 (MQ=255)
                                                                    aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAAc                                                               <  2:291741/139‑1 (MQ=255)
                                                                    aacaaAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAAc                                                               <  2:277297/139‑1 (MQ=255)
                                                                        aaaCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACgctg                                                                                        >  1:717677/1‑110 (MQ=255)
                                                                        aaaCGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACgctg                                                                                        <  2:717677/110‑1 (MQ=255)
                                                                           cggcggATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGt                                                        >  1:1280331/1‑139 (MQ=255)
                                                                                gATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGcc                                                   <  2:963526/139‑1 (MQ=255)
                                                                                gATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGcc                                                   <  1:1441007/139‑1 (MQ=255)
                                                                                   aaGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTg                                                >  2:796039/1‑139 (MQ=255)
                                                                                                  cGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCg                                 <  2:890468/139‑1 (MQ=255)
                                                                                                               tGTAGTGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGcc                    >  1:1151434/1‑139 (MQ=255)
                                                                                                                    tGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGa                                                                                             <  2:420006/61‑1 (MQ=255)
                                                                                                                    tGGTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGa                                                                                             >  1:420006/1‑61 (MQ=255)
                                                                                                                     ggTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCa                                       <  1:80695/114‑1 (MQ=255)
                                                                                                                     ggTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCa                                       >  2:80695/1‑114 (MQ=255)
                                                                                                                     ggTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCga                                <  2:1412499/121‑1 (MQ=255)
                                                                                                                     ggTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCga                                >  1:1412499/1‑121 (MQ=255)
                                                                                                                     ggTTCATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCActgct              >  2:480692/1‑139 (MQ=255)
                                                                                                                         cATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAAc                                                               >  1:568976/1‑86 (MQ=255)
                                                                                                                         cATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAAc                                                               <  2:568976/86‑1 (MQ=255)
                                                                                                                         cATCGAGAATAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCAc          >  2:734822/1‑139 (MQ=255)
                                                                                                                                 aTAAGCAGCGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGttttt  <  1:734822/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GCAGGCGCATCTTCAGCGTGGTGCGAAACAAACAACAATTGCGTTTCACCTTCGCTAATCAGCACATCAACAAAACGGCGGATAAGCTGGCGATTCAGCGGATCAAGCCCCTGTAGTGGTTCATCGAGAATAAGCAACGTCGGATGTTTCACCAGTGCGCGGACAATCAGCGCCAGACGCTGCTGTCCCCAGGAAAGACTATGGAACGGAGCGTCAGCCGTGCGTTTATCAATGCCGAGAATATCCAGCCACTGCTGCACCAGTTTTT  >  NC_000913/792389‑792656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑