Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	902,473	G→A	F172F (TTC→TTT)	artI ←	arginine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	902,473	0	G	A	100.0%	201.4 / NA	58	F172F (TTC→TTT)	artI	arginine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

TGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCGAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTGTCGTCCCGTTCTGTACGCCGACTTTT  >  NC_000913/902339‑902608                                                                                                                                       |                                                                                                                                        tgcAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGa                                                                                                                                     <  2:1479259/139‑1 (MQ=255)               gCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGc                                                                                                                       <  1:343158/139‑1 (MQ=255)                     aCGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGttt                                                                                                                 >  2:1049999/1‑139 (MQ=255)                     aCGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGttt                                                                                                                 >  2:175062/1‑139 (MQ=255)                         aCCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCa                                                                                                             >  1:946304/1‑139 (MQ=255)                          ccGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAg                                                                                                            >  1:63939/1‑139 (MQ=255)                                          aGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTc                                                                                            >  2:224097/1‑139 (MQ=255)                                           gTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCt                                                                                           <  2:946304/139‑1 (MQ=255)                                            tGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGc                                                                                                                       <  2:321412/110‑1 (MQ=255)                                            tGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGc                                                                                                                       >  1:321412/1‑110 (MQ=255)                                            tGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTg                                                                                          >  1:1213766/1‑139 (MQ=255)                                                gAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAg                                                                                      <  2:845355/139‑1 (MQ=255)                                                gAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAg                                                                                      <  2:290662/139‑1 (MQ=255)                                                   gTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTg                                                                                   <  1:336316/139‑1 (MQ=255)                                                      aTCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCa                                                                                >  2:1003371/1‑139 (MQ=255)                                                        cTTTATCGGTCACTTTGCCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATa                                                                              <  1:928985/139‑1 (MQ=255)                                                           tATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACgg                                                                           <  1:175062/139‑1 (MQ=255)                                                           tATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACgg                                                                           <  1:1049999/139‑1 (MQ=255)                                                              cGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCa                                                                                                       >  1:60096/1‑108 (MQ=255)                                                              cGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCa                                                                                                       <  2:60096/108‑1 (MQ=255)                                                                gTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACgg                                                                      <  1:1069925/139‑1 (MQ=255)                                                                  cACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACgg                                                                           <  2:252010/132‑1 (MQ=255)                                                                  cACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACgg                                                                           >  1:252010/1‑132 (MQ=255)                                                                   aCTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGc                                                                                                              >  1:360028/1‑96 (MQ=255)                                                                   aCTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGc                                                                                                              <  2:360028/96‑1 (MQ=255)                                                                       tGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGAt                                                               >  1:868989/1‑139 (MQ=255)                                                                           gCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGa                                                                                                                                     >  2:992732/1‑65 (MQ=255)                                                                           gCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGa                                                                                                                                     <  1:992732/65‑1 (MQ=255)                                                                            cccACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCg                                                          <  2:901084/139‑1 (MQ=255)                                                                               accaccGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGc                                                                                                                       >  2:729683/1‑75 (MQ=255)                                                                               accaccGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGc                                                                                                                       <  1:729683/75‑1 (MQ=255)                                                                                          tttCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAg                                                                   <  2:1089940/116‑1 (MQ=255)                                                                                          tttCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAg                                                                   >  1:1089940/1‑116 (MQ=255)                                                                                                  tATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAAttt                                    <  2:1213766/139‑1 (MQ=255)                                                                                                     cTTTCAGCCACTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTg                                 <  2:63939/139‑1 (MQ=255)                                                                                                               cTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATa                                                                              >  2:82823/1‑84 (MQ=255)                                                                                                               cTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATa                                                                              <  1:82823/84‑1 (MQ=255)                                                                                                               cTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTgtcgtc                       >  1:783310/1‑139 (MQ=255)                                                                                                               cTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTgtcgtc                       >  2:628652/1‑139 (MQ=255)                                                                                                               cTCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTgtcgtc                       >  2:456927/1‑139 (MQ=255)                                                                                                                tCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTAt                                                 <  2:1182470/112‑1 (MQ=255)                                                                                                                tCAGTGACCACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTAt                                                 >  1:1182470/1‑112 (MQ=255)                                                                                                                        cACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCggg                                                        <  1:1054749/97‑1 (MQ=255)                                                                                                                        cACTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCggg                                                        >  2:1054749/1‑97 (MQ=255)                                                                                                                          cTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGtt                                                                                             <  2:313372/58‑1 (MQ=255)                                                                                                                          cTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGtt                                                                                             >  1:313372/1‑58 (MQ=255)                                                                                                                          cTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGgt                               <  1:277186/120‑1 (MQ=255)                                                                                                                          cTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGgt                               >  2:277186/1‑120 (MQ=255)                                                                                                                          cTGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTGTCGTCCCGTTCTGTAc            >  1:1074888/1‑139 (MQ=255)                                                                                                                           tGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTAt                                                 <  2:451765/101‑1 (MQ=255)                                                                                                                           tGCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTAt                                                 >  1:451765/1‑101 (MQ=255)                                                                                                                            gCGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTGTCGTCCCGTTCTGTACGc          >  1:288888/1‑139 (MQ=255)                                                                                                                             cGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGgtg                              <  2:917973/118‑1 (MQ=255)                                                                                                                             cGGTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGgtg                              >  1:917973/1‑118 (MQ=255)                                                                                                                              ggTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATa                                            <  2:493523/103‑1 (MQ=255)                                                                                                                              ggTGTCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATa                                            >  1:493523/1‑103 (MQ=255)                                                                                                                                  tCACCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTGTCGTCCCGTTCTGTACGCCGACtt    >  2:786932/1‑139 (MQ=255)                                                                                                                                    aCCAAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTGTCGTCCCGTTCTGTACGCCGACtttt  <  2:1343249/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCAGCTCAGTGTTGCCCTGACGTACCGCGATGCCGAGGCCAGTGCCGAAGTAATCTTTATCGGTCACTTTGTCGCCCACCGCCGCCAGTTTCGGGTTATCTTTCAGCCACTCAGTGACCACTGCGGTGTCACCGAAGACGCCGTCGATACGCCCGTTTTGCAGATCCAGTTTTGCGTTCTGGTAGCTGTCATACGGAACGGTAGTGATTTCCGGGTGCTTATCCATAATGAATTTCTGGTGTGTCGTCCCGTTCTGTACGCCGACTTTT  >  NC_000913/902339‑902608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑