Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,030,407	A→G	F88L (TTT→CTT)	tusE ←	mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,030,407	0	A	G	100.0%	183.8 / NA	50	F88L (TTT→CTT)	tusE	mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/29);  total (21/29)

CGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCA  >  NC_000913/1030272‑1030539                                                                                                                                        |                                                                                                                                     cgcgTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   >  2:55351/1‑139 (MQ=255) cgcgTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   <  2:547891/139‑1 (MQ=255) cgcgTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   <  1:746818/139‑1 (MQ=255)   cgTGTAGAACGGGAGCTCAGCCAGCCGCATCATCCCTCGGGGGAATGAACCGATTATCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTAGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTc                                                                                                                                 <  2:153387/139‑1 (MQ=255)      gtAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGt                                                                                                                              <  2:610109/139‑1 (MQ=255)              ggTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAg                                                                                                                      <  1:55351/139‑1 (MQ=255)                    cAGCGAGCCGCGTCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTg                                                                                                                <  1:978455/139‑1 (MQ=255)                    cAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTg                                                                                                                >  2:1132773/1‑139 (MQ=255)                    cAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTg                                                                                                                <  1:1147504/139‑1 (MQ=255)                               atcatcCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   <  2:804560/109‑1 (MQ=255)                               atcatcCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   >  1:804560/1‑109 (MQ=255)                                         gggggAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGt                                                                                                                                  <  1:149073/100‑1 (MQ=255)                                         gggggAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGt                                                                                                                                  >  2:149073/1‑100 (MQ=255)                                         gggggAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAAt                                                                                           >  1:1263030/1‑139 (MQ=255)                                                ttAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCg                                                                                    >  1:251810/1‑139 (MQ=255)                                                   aCCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                                                                 <  1:1132773/139‑1 (MQ=255)                                                   aCCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                                                                 <  1:1285300/139‑1 (MQ=255)                                                              tATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTAcc                                                                      <  1:113713/139‑1 (MQ=255)                                                                    ttAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATa                                                                >  1:56921/1‑139 (MQ=255)                                                                         tACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAAt                                                           <  1:333256/139‑1 (MQ=255)                                                                            aTTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCTGGACCTTTCGGAAGCAGTc                                                                                                                                 <  1:329463/66‑1 (MQ=255)                                                                            aTTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCTGGACCTTTCGGAAGCAGTc                                                                                                                                 >  2:329463/1‑66 (MQ=255)                                                                                        ttGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTAtt                                            >  2:150903/1‑139 (MQ=255)                                                                                        ttGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTAtt                                            <  2:106796/139‑1 (MQ=255)                                                                                          ggCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAAt                                                           >  2:57098/1‑122 (MQ=255)                                                                                          ggCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAAt                                                           <  1:57098/122‑1 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   <  2:37920/45‑1 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAg                                                                                                                                   >  1:37920/1‑45 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTc                                                                                                                                 <  1:286836/47‑1 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTc                                                                                                                                 >  2:286836/1‑47 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                                                                 >  1:1361823/1‑95 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCa                                                                                 <  2:1361823/95‑1 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag                                                   <  1:1130103/125‑1 (MQ=255)                                                                                               gCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag                                                   >  2:1130103/1‑125 (MQ=255)                                                                                                               gcttgcttAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGca                     >  2:439844/1‑139 (MQ=255)                                                                                                                 ttgcttAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTCCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAAt                                                           <  1:669130/99‑1 (MQ=255)                                                                                                                 ttgcttAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTACGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAAt                                                           >  2:669130/1‑99 (MQ=255)                                                                                                                      tAGCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACg              <  2:56921/139‑1 (MQ=255)                                                                                                                        gCCGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGca            <  1:150903/139‑1 (MQ=255)                                                                                                                          cGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag                                                   <  1:1276519/98‑1 (MQ=255)                                                                                                                          cGGACCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGgag                                                   >  2:1276519/1‑98 (MQ=255)                                                                                                                             aCCTTTCTGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTAc                                                                       <  1:939192/75‑1 (MQ=255)                                                                                                                             aCCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTAc                                                                       >  2:939192/1‑75 (MQ=255)                                                                                                                             aCCTTTCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACt       <  2:1263030/139‑1 (MQ=255)                                                                                                                                 ttCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTttct                                                                                                    >  1:881405/1‑42 (MQ=255)                                                                                                                                 ttCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTttct                                                                                                    <  2:881405/42‑1 (MQ=255)                                                                                                                                  tCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCa  <  2:251810/139‑1 (MQ=255)                                                                                                                                  tCGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCa  >  2:141079/1‑139 (MQ=255)                                                                                                                                   cGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCg                                                      >  1:145821/1‑86 (MQ=255)                                                                                                                                   cGGAAGCAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCg                                                      <  2:145821/86‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCGTGTAGAACGGGTGCTCAGCGAGCCGCATCATCCCTCGGGGGAATTAACCGATTTTCGTATCCGTTAAATACATTTTACCGGTTTTGGCAGGCCAGCAATTTTGGTGGCTTGCTTAGCCGGACCTTTCGGAAACAGTCGGTATAAATAGCGGCTGTTACCTTTTTCTTCGCCAAATTTATTCGCCATCGCTTTTACCAGCATACGAATCGCCGGAGAAGTATTGAATTCCAGATAGAAATCACGCACAAAACGCACCACTTCCCA  >  NC_000913/1030272‑1030539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑