Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,037,227	C→T	P160L (CCC→CTC)	hyaF →	hydrogenase‑1 protein nickel incorporation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,037,227	0	C	T	100.0%	131.7 / NA	40	P160L (CCC→CTC)	hyaF	hydrogenase‑1 protein nickel incorporation factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

CGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCA  >  NC_000913/1037093‑1037360                                                                                                                                       |                                                                                                                                      cGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCAtt                                                                                                                                   >  1:1186179/1‑139 (MQ=255)    aTTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAAACTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGAt                                                                                                                                >  2:201016/1‑139 (MQ=255)        tGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGcc                                                                                                                            >  1:114268/1‑139 (MQ=255)        tGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGcc                                                                                                                            >  1:1243557/1‑139 (MQ=255)          gCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtg                                                                                                                          <  2:137669/139‑1 (MQ=255)              gTGCGCAGACGTCGCGGCGAAAAGTTGCTGGGGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCCGCAACGCAAAATCCCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCcgagta                                                                                                                      >  2:609475/1‑136 (MQ=255)              gTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGa                                                                                                                                 <  2:156674/128‑1 (MQ=255)              gTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGa                                                                                                                                 >  1:156674/1‑128 (MQ=255)              gTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtgatga                                                                                                                      >  1:1344532/1‑139 (MQ=255)              gTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtgatga                                                                                                                      <  1:914985/139‑1 (MQ=255)                       cGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtg                                                                                                                          <  2:260806/126‑1 (MQ=255)                       cGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtg                                                                                                                          >  1:260806/1‑126 (MQ=255)                          cgcgGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGt                                                                                                          <  2:855028/139‑1 (MQ=255)                          cgcgGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGt                                                                                                          <  2:34307/139‑1 (MQ=255)                               cgAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGgcg                                                                                                     <  1:609475/139‑1 (MQ=255)                                          tGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACt                                                                                          <  2:114268/139‑1 (MQ=255)                                            gaggaCAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTgg                                                                                        <  2:1344532/139‑1 (MQ=255)                                                 cAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACAt                                                                                   <  2:592639/139‑1 (MQ=255)                                                   aaCTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGt                                                                                 >  1:784237/1‑139 (MQ=255)                                                    aCTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTa                                                                                <  2:684963/139‑1 (MQ=255)                                                    aCTGGAGGCTGGCTGCGCGCCGCTGGAGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTgg                                                                                        >  1:1421891/1‑131 (MQ=255)                                                    aCTGGAGGCTGGCTGCGCGCCGCTGGAGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTgg                                                                                        <  2:1421891/131‑1 (MQ=255)                                                         aGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATg                                                                                                                               >  2:1065913/1‑87 (MQ=255)                                                         aGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATg                                                                                                                               <  1:1065913/87‑1 (MQ=255)                                                         aGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTaa                                                                           >  1:774503/1‑139 (MQ=255)                                                          ggctggctGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAAc                                                                          >  2:729657/1‑139 (MQ=255)                                                              ggctgCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCg                                                                      >  1:1189748/1‑139 (MQ=255)                                                                ctgCGCGCCGCTGGCGTTGTGGCAGGCGTCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCACCGCTCATTGATGGCCTGATGAATGGc                                                                                                                 >  1:1081344/1‑94 (MQ=255)                                                                ctgCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGAc                                                                    >  2:1277905/1‑139 (MQ=255)                                                                ctgCGCGCCGCTGGCGTAGTGGCAGGCGTCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCACCGCTCATTGATGGCCTGATGAATGGc                                                                                                                 <  2:1081344/94‑1 (MQ=255)                                                                  gcgcgcCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtgatga                                                                                                                      >  2:160633/1‑87 (MQ=255)                                                                  gcgcgcCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCtgatga                                                                                                                      <  1:160633/87‑1 (MQ=255)                                                                    gcgcCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACgcgc                                                                <  2:1189748/139‑1 (MQ=255)                                                                     cgccgcTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCa                                                               >  2:1396853/1‑139 (MQ=255)                                                                           tGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGca                                                         <  2:1243557/139‑1 (MQ=255)                                                                                  gtgGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCAtt                                                  >  1:327030/1‑139 (MQ=255)                                                                                         gcggcAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGa                                           <  2:327030/139‑1 (MQ=255)                                                                                          cggcAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGAc                                          >  2:229681/1‑139 (MQ=255)                                                                                                    aaaTCTCTTGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTAccc                                <  1:125260/139‑1 (MQ=255)                                                                                                           ttGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCaa                                      <  1:370112/126‑1 (MQ=255)                                                                                                           ttGCCGACAGATTCGCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCaa                                      >  2:370112/1‑126 (MQ=255)                                                                                                                         gCTGTTACCGCCGCTCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCtt           <  1:229681/139‑1 (MQ=255)                                                                                                                                  gccgctCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa  <  1:729657/139‑1 (MQ=255)                                                                                                                                  gccgctCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCa  <  1:1396853/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CGGATTATGGCGGGTGCGCAGACGTCGCGGCGAAAAGTTGCTGGAGGACAAACTGGAGGCTGGCTGCGCGCCGCTGGCGTTGTGGCAGGCGGCAACGCAAAATCTCTTGCCGACAGATTCGCTGTTACCGCCGCCCATTGATGGCCTGATGAATGGCCTACCGTTGGCGCATGAGTTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAGCATTAATCTGACGCAATTACCCATCAGCGAGGCTGATCGGCTTTTTCTCTCA  >  NC_000913/1037093‑1037360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑