Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,043,741 A→G V157A (GTT→GCT)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,043,7410AG100.0% 239.4 / NA 65V157A (GTT→GCT) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/34);  total (31/34)

AGGACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAACCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGCTCA  >  NC_000913/1043606‑1043872
                                                                                                                                       |                                                                                                                                   
aGGACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCg                                                                                                                                  <  2:1493956/139‑1 (MQ=255)
   aCAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGa                                                                                                                               <  1:674851/139‑1 (MQ=255)
     aaaCTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAgt                                                                                                                             <  1:634749/139‑1 (MQ=255)
        cTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGATGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTc                                                                                                                          <  1:908112/139‑1 (MQ=255)
         tgtgTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCa                                                                                                                         <  2:730412/139‑1 (MQ=255)
          gtgtTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAg                                                                                                                        <  1:1494981/139‑1 (MQ=255)
             ttCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTg                                                                                                                     <  1:1501033/139‑1 (MQ=255)
                 tGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTGACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGc                                                                                                                    <  2:386351/136‑1 (MQ=255)
                 tGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTGACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGc                                                                                                                    >  1:386351/1‑136 (MQ=255)
                  ggTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGAt                                                                                                                >  1:281021/1‑139 (MQ=255)
                  ggTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTATCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGAt                                                                                                                >  2:1066257/1‑139 (MQ=255)
                       ggCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCCTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTc                                                                                                                          >  1:1077063/1‑124 (MQ=255)
                       ggCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCCTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTc                                                                                                                          <  2:1077063/124‑1 (MQ=255)
                          cTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCa                                                                                                        <  2:281021/139‑1 (MQ=255)
                            tAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                      <  1:1066257/139‑1 (MQ=255)
                            tAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                      <  2:184133/139‑1 (MQ=255)
                                  ccGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCt                                                                                                             <  1:1263305/126‑1 (MQ=255)
                                  ccGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCt                                                                                                             >  2:1263305/1‑126 (MQ=255)
                                        tAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAat                                                                                          <  2:124492/139‑1 (MQ=255)
                                                  aaCGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTgg                                                                                             >  2:995573/1‑126 (MQ=255)
                                                  aaCGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTgg                                                                                             <  1:995573/126‑1 (MQ=255)
                                                  aaCGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAg                                                                                >  2:181193/1‑139 (MQ=255)
                                                    cGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTg                                                                                                                     <  1:1098575/100‑1 (MQ=255)
                                                    cGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTg                                                                                                                     >  2:1098575/1‑100 (MQ=255)
                                                                   gACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATcc                                                                                                              <  1:653951/92‑1 (MQ=255)
                                                                   gACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATcc                                                                                                              >  2:653951/1‑92 (MQ=255)
                                                                        tGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGtt                                                          <  2:904781/139‑1 (MQ=255)
                                                                                              tGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAg                                                                                                   <  2:1380841/76‑1 (MQ=255)
                                                                                              tGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAg                                                                                                   >  1:1380841/1‑76 (MQ=255)
                                                                                              tGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAcc                                                               >  1:1199617/1‑112 (MQ=255)
                                                                                              tGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCAcc                                                               <  2:1199617/112‑1 (MQ=255)
                                                                                                  ctctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCcg                                <  1:362111/139‑1 (MQ=255)
                                                                                                    ctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCt                                                                                                             >  1:510472/1‑60 (MQ=255)
                                                                                                    ctTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCt                                                                                                             <  2:510472/60‑1 (MQ=255)
                                                                                                      tCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGa                                                                                                                               >  1:160727/1‑40 (MQ=255)
                                                                                                      tCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGa                                                                                                                               <  2:160727/40‑1 (MQ=255)
                                                                                                            tCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                      <  1:70789/59‑1 (MQ=255)
                                                                                                            tCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCAtt                                                                                                      >  2:70789/1‑59 (MQ=255)
                                                                                                            tCCAGTGTATAGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACaa                      <  1:181193/139‑1 (MQ=255)
                                                                                                                      aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGcc                                      >  2:378690/1‑113 (MQ=255)
                                                                                                                      aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGcc                                      <  1:378690/113‑1 (MQ=255)
                                                                                                                      aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATa            >  2:658467/1‑139 (MQ=255)
                                                                                                                      aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATa            >  1:816839/1‑139 (MQ=255)
                                                                                                                      aGTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATa            >  1:1160198/1‑139 (MQ=255)
                                                                                                                       gTGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATAc           <  2:1160198/139‑1 (MQ=255)
                                                                                                                        tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTg                                                                             <  2:1457011/72‑1 (MQ=255)
                                                                                                                        tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTg                                                                             >  1:1457011/1‑72 (MQ=255)
                                                                                                                        tGGCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACt          >  1:1513074/1‑139 (MQ=255)
                                                                                                                         ggCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACtt         >  2:7128/1‑139 (MQ=255)
                                                                                                                          gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACGTGGTTTTTCTTTGGTTAATCTCGcc                                      >  1:801901/1‑109 (MQ=255)
                                                                                                                          gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGcc                                      <  2:801901/109‑1 (MQ=255)
                                                                                                                          gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACtt         >  2:215495/1‑138 (MQ=255)
                                                                                                                          gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACtt         <  1:215495/138‑1 (MQ=255)
                                                                                                                          gCCGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTc        >  1:1270057/1‑139 (MQ=255)
                                                                                                                           ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATAc           >  2:856746/1‑135 (MQ=255)
                                                                                                                           ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATAc           <  1:856746/135‑1 (MQ=255)
                                                                                                                           ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACtt         <  1:1424967/137‑1 (MQ=255)
                                                                                                                           ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCt       >  1:98524/1‑139 (MQ=255)
                                                                                                                           ccGTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCGCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACtt         >  2:1424967/1‑137 (MQ=255)
                                                                                                                             gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAat                                                                                          >  1:807303/1‑54 (MQ=255)
                                                                                                                             gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAat                                                                                          <  2:807303/54‑1 (MQ=255)
                                                                                                                             gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATcc                                                                                  <  2:417153/62‑1 (MQ=255)
                                                                                                                             gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATcc                                                                                  >  1:417153/1‑62 (MQ=255)
                                                                                                                             gTTTTCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGc     >  1:70734/1‑139 (MQ=255)
                                                                                                                                ttCCCCAGCCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGCTCa  >  1:1439482/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                   
AGGACAAACTGTGTTCCTGGTTTGGCCTTAATGTCCGCGATAGTCAGCGCAACGCCATCTTTTTCCAGACGCTGGCCGACCATACCATTGACGGTGAACTCTTCACCTTCCAGTGTATAGTGGCCGTTTTCCCCAACCGTGAGTGTCAGTTGCTGATCCTGACCATTCAGTTGTGGAATATGCATCCAGCTGATCGCCAGCTCACCTGGTTTTTCTTTGGTTAATCTCGCCCAGCCGCGACCCACAATCGGAAAATACTTCTGCTCA  >  NC_000913/1043606‑1043872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑