Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,044,364	T→C	T105A (ACA→GCA)	etp ←	O‑antigen capsule forming protein‑tyrosine‑phosphatase, Etk‑P dephosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,044,364	0	T	C	100.0%	168.8 / NA	46	T105A (ACA→GCA)	etp	O‑antigen capsule forming protein‑tyrosine‑phosphatase, Etk‑P dephosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

CCGGCTGAGGCGCTTCGCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGTCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCAT  >  NC_000913/1044233‑1044498                                                                                                                                    |                                                                                                                                       ccGGCTGAGGCGCTTCGCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCcg                                                                                                                                 <  1:929449/139‑1 (MQ=255)           cgcTTCGCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGAc                                                                                                                            >  2:56754/1‑134 (MQ=255)           cgcTTCGCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGAc                                                                                                                            <  1:56754/134‑1 (MQ=255)                 gCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCaa                                                                                                                 <  2:502990/139‑1 (MQ=255)                 gCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCaa                                                                                                                 <  2:843437/139‑1 (MQ=255)                      ttCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCg                                                                                                            <  1:464707/139‑1 (MQ=255)                             cTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCt                                                                                                     <  1:525651/139‑1 (MQ=255)                                 cgcgcTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGc                                                                                                 >  2:720690/1‑139 (MQ=255)                                     cTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGt                                                                                                          <  2:1319332/126‑1 (MQ=255)                                     cTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGt                                                                                                          >  1:1319332/1‑126 (MQ=255)                                                cGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCaa                                                                                                                 >  2:76311/1‑108 (MQ=255)                                                cGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCaa                                                                                                                 <  1:76311/108‑1 (MQ=255)                                                  tAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCa                                                                                >  2:1164395/1‑139 (MQ=255)                                                  tAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCa                                                                                >  2:1326716/1‑139 (MQ=255)                                                            cAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAAt                                                                      <  1:720690/139‑1 (MQ=255)                                                              aaTGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCa                                                                    <  2:1291934/139‑1 (MQ=255)                                                              aaTGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCa                                                                    <  1:7072/139‑1 (MQ=255)                                                               aTGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAg                                                                   >  1:1221948/1‑139 (MQ=255)                                                                    tCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGt                                                              >  1:794009/1‑139 (MQ=255)                                                                    tCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGt                                                              <  2:1405707/139‑1 (MQ=255)                                                                    tCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGt                                                              >  2:1444947/1‑139 (MQ=255)                                                                            tttACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGt                                                      <  2:794009/139‑1 (MQ=255)                                                                                   tAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCAtct                                               >  2:1189807/1‑139 (MQ=255)                                                                                   tAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCAtct                                               >  2:390850/1‑139 (MQ=255)                                                                                     gggATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGAc                                                                                    >  2:294364/1‑100 (MQ=255)                                                                                     gggATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGAc                                                                                    <  1:294364/100‑1 (MQ=255)                                                                                     gggATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTca                                             >  2:457141/1‑139 (MQ=255)                                                                                              gATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGc                                    >  2:1373264/1‑139 (MQ=255)                                                                                                 ctctTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGa                                                                                                   <  2:282023/73‑1 (MQ=255)                                                                                                 ctctTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGa                                                                                                   >  1:282023/1‑73 (MQ=255)                                                                                                  tctTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTcagca                                          <  2:541078/129‑1 (MQ=255)                                                                                                  tctTTCTGTTCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTcagca                                          >  1:541078/1‑129 (MQ=255)                                                                                                          ttCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGa                                                                                                   <  2:1370538/64‑1 (MQ=255)                                                                                                          ttCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGa                                                                                                   >  1:1370538/1‑64 (MQ=255)                                                                                                          ttCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCa                        <  1:1094470/139‑1 (MQ=255)                                                                                                           tCCAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCAt                       <  1:849109/139‑1 (MQ=255)                                                                                                            ccAGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATg                      <  1:390850/139‑1 (MQ=255)                                                                                                              aGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGc                                                    >  1:995180/1‑107 (MQ=255)                                                                                                              aGCCATTGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATAGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGc                                                    <  2:995180/107‑1 (MQ=255)                                                                                                                    tGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCg                                                               <  2:1447969/90‑1 (MQ=255)                                                                                                                    tGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCg                                                               >  1:1447969/1‑90 (MQ=255)                                                                                                                    tGCCCAAACAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCaa              >  2:1290989/1‑139 (MQ=255)                                                                                                                            cAGCATTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACg      <  2:928951/139‑1 (MQ=255)                                                                                                                                aTTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCa                        >  2:200092/1‑117 (MQ=255)                                                                                                                                aTTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCa                        <  1:200092/117‑1 (MQ=255)                                                                                                                                aTTGCCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCAt  >  2:1263289/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                       CCGGCTGAGGCGCTTCGCCCATTCCTGACTGGCGCGCTCCAACATACCGTAGACATGTTCAAATGCGTCCTGACTTTTACGATAGGGATCCGGGATCTCTTTCTGTTCCAGCCATTGCCCAAACAGCATTGTCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCAT  >  NC_000913/1044233‑1044498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑